Detection of the adulteration of camel milk powder with cow milk by ultra-high performance liquid chromatography (UPLC)

Li, Lingyu; Wang, Jun; Li, Minjing; Yang, Yingchun; Wang, Zhixuan; Miao, Jing; Zhao, Zhongkai; Yang, Jie

doi:10.1016/j.idairyj.2021.105117

Cited by 22 publications

(10 citation statements)

References 40 publications

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“…If we restrict to only a set of distinct concentrations (20, 40, 60, 80%) with wide separation intervals, the RMSE for a full set of samples can also drop from 11.4 to 4.63% and from 17.0 to 9.15% in caprine and ovine milk samples, respectively. Hence, the inclusion of possible influences acting on the composition of the milk proteome in the development of the regression model is necessary to achieve adequate reliability and robustness of methodology, which is also supported by other published studies (Czerwenka et al, 2010;Trimboli et al, 2019;Li et al, 2021).…”

Section: Maldi-tof Ms As a Tool For Detection Of Small Ruminant Milk ...mentioning

confidence: 80%

Use of MALDI-TOF MS technology to evaluate adulteration of small ruminant milk with raw bovine milk

Rysová

Cejnar

Hanuš

et al. 2022

Journal of Dairy Science

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Detection of adulteration of small ruminant milk is very important for health and commercial reasons. New analytical and cost-effective methods need to be developed to detect new adulteration practices. In this work, we aimed to explore the ability of the MALDI-TOF mass spectrometry to detect bovine milk in caprine and ovine milk using samples from 18 dairy farms. Different levels of adulteration (0.5, 1, 5, 10, 20, 40, 60, and 80%) were analyzed during the lactation period of goat and sheep (in May, from 60 to 90 d in milk, and in August, from 150 to 180 d in milk). Two different ranges of peptide-protein spectra (500-4,000 Da; 4-20 kDa) were used to establish a calibration model for predicting the concentration of adulterant using partial least squares and generalized linear model with lasso regularization. The low molecular weight part of the spectra together with the generalized linear model with lasso regularization regression model appeared to have greater potential for our aim of detection of adulteration of small ruminants' milk. The subsequent prediction model was able to predict the concentration of bovine milk in caprine milk with a root mean square error of 11.4 and 17.0% in ovine milk. The results offer compelling evidence that MALDI-TOF can detect the adulteration of small ruminants' milk. However, the method is severely limited by (1) the complexity of the milk proteome resulting from the adulteration technique, (2) the potential degradation of thermolabile proteins, and (3) the genetic variability of tested samples. Additionally, the root mean square error of prediction based only on one individual sample adulteration series can drop down to 6.34% for quantification of adulterated caprine milk and 6.28% for adulterated ovine milk for the full set of concentrations or down to 2.33 and 4.00%, respectively, if we restrict only to low concentrations of adulteration (0, 0.5, 1, 5, 10%).

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Section: Maldi-tof Ms As a Tool For Detection Of Small Ruminant Milk ...mentioning

confidence: 80%

Use of MALDI-TOF MS technology to evaluate adulteration of small ruminant milk with raw bovine milk

Rysová

Cejnar

Hanuš

et al. 2022

Journal of Dairy Science

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“…This reduction was attributed to protein degradation during the cheesemaking process. In a recent study, β-lactoglobulin was employed to detect adulteration of camel milk powder with BM at levels as low as 5 %, using ultra-high performance liquid chromatography ( Li et al, 2021 ). As discussed previously, the differences in amino acid sequences and physicochemical properties of β-lactoglobulin contribute to its use as a fingerprint protein for detecting milk adulteration.…”

Section: Discussionmentioning

confidence: 99%

Identification of pasteurized mare milk and powder adulteration with bovine milk using quantitative proteomics and metabolomics approaches

Ji,

Zhang,

Deng

et al. 2024

Food Chemistry: X

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“…UHPLC has also been employed to detect adulterated milk powder samples, with an acceptable linear correlation coefficient. It is used to assess food products and their diversity or assure their quality and authenticity [69,70]. Quantification of essential nutrients from human milk was quantified by UHPLC to direct the diet for lactating women [72].…”

Section: Food Safetymentioning

confidence: 99%

Principles and Applications of Ultra-High-Performance Liquid Chromatography

Ahmed¹,

Eticha²,

Hymete³

et al. 2023

High Performance Liquid Chromatography - Recent Advances and Applications

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The science of separation had advanced significantly with the development of ultra-high-performance liquid chromatography (UHPLC), a brand-new type of liquid chromatography. The need for the evolution of HPLC into UHPLC has been driven by the continuously evolving of packing material modifications that affect the separation of mixtures. The separation process of analytes is completed in a substantially decreased amount of time due to the lower particle sizes, which increases surface area of interaction allowing reduction of column length to one-third; thus, shorter columns are employed in UHPLC, which consequently causes the flow rate to be three times higher and subsequently reducing analysis time. Although UHPLC shares the same fundamental idea and instrument layout as HPLC, it differs from HPLC in that it produces narrow peaks and has high spectral quality, allowing for simple compound identification in a variety of analytical applications such as impurity profiling, product formulation, and improved analytical technique and method development. However, high back pressure in UHPLC might lead to decreased column life, and the instrument’s higher price compared to HPLC are the disadvantages.

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Detection of the adulteration of camel milk powder with cow milk by ultra-high performance liquid chromatography (UPLC)

Cited by 22 publications

References 40 publications

Use of MALDI-TOF MS technology to evaluate adulteration of small ruminant milk with raw bovine milk

Use of MALDI-TOF MS technology to evaluate adulteration of small ruminant milk with raw bovine milk

Identification of pasteurized mare milk and powder adulteration with bovine milk using quantitative proteomics and metabolomics approaches

Principles and Applications of Ultra-High-Performance Liquid Chromatography

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