Detection of THCA in Oral Fluid by GC-MS-MS

Day, David A.; Kuntz, D; Feldman, Michaël; Presley, Lance

doi:10.1093/jat/30.9.645

Cited by 51 publications

(52 citation statements)

References 11 publications

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“…The presence of low concentrations of THCCOOH and conjugated-THCCOOH in oral fluid cannot be accounted for by cannabis smoke deposition as studies have not detected these analytes in cannabis smoke (19,31). In contrast to the prior study by Perez-Reyes (3), we believe it is likely that these metabolites in oral fluid arise from diffusion from blood into oral fluid.…”

Section: Discussioncontrasting

confidence: 68%

Nonsmoker Exposure to Secondhand Cannabis Smoke. III. Oral Fluid and Blood Drug Concentrations and Corresponding Subjective Effects

et al. 2015

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The increasing use of highly potent strains of cannabis prompted this new evaluation of human toxicology and subjective effects following passive exposure to cannabis smoke. The study was designed to produce extreme cannabis smoke exposure conditions tolerable to drugfree nonsmokers. Six experienced cannabis users smoked cannabis cigarettes [5.3% D 9 -tetrahydrocannabinol (THC) in Session 1 and 11.3% THC in Sessions 2 and 3] in a closed chamber. Six nonsmokers were seated alternately with smokers during exposure sessions of 1 h duration. Sessions 1 and 2 were conducted with no ventilation and ventilation was employed in Session 3. Oral fluid, whole blood and subjective effect measures were obtained before and at multiple time points after each session. Oral fluid was analyzed by ELISA (4 ng/mL cutoff concentration) and by LC-MS-MS (limit of quantitation) for THC (1 ng/mL) and total THCCOOH (0.02 ng/mL). Blood was analyzed by LC -MS-MS (0.5 ng/mL) for THC, 11-OH-THC and free THCCOOH. Positive tests for THC in oral fluid and blood were obtained for nonsmokers up to 3 h following exposure. Ratings of subjective effects correlated with the degree of exposure. Subjective effect measures and amounts of THC absorbed by nonsmokers (relative to smokers) indicated that extreme secondhand cannabis smoke exposure mimicked, though to a lesser extent, active cannabis smoking.

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Section: Discussioncontrasting

confidence: 68%

Nonsmoker Exposure to Secondhand Cannabis Smoke. III. Oral Fluid and Blood Drug Concentrations and Corresponding Subjective Effects

et al. 2015

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“…Des prélèvements salivaires effectués lors d'étu-des [25,41,42] ont permis de montrer que la concentration en THC et THCCOOH dans la salive varie généralement entre 0,5 et 200 ng/mL pour le THC, entre 10 et 100 pg/mL pour le THCCOOH libre et entre 100 et 300 pg/mL pour le THCCOOH total (libre + conjugué). Cependant, certains échantillons prélevés peu de temps après avoir fumé peuvent dépasser 350 ng de THC/mL [42].…”

Section: Volume D'échantillon Et Concentrations Habituellement Mesuréesunclassified

“…Certains auteurs l'utilisent également pour les cheveux [28,29,32] ou le fluide oral [41,[62][63][64].…”

Section: Extraction Sur Phase Solideunclassified

“…Pourtant, la dérivation n'est pas toujours nécessaire pour l'analyse de certains cannabinoïdes et des auteurs choisissent de les analyser directement après l'extraction en GC-MS/MS [18,26,27,41].…”

Section: Dérivationunclassified

“…Deux transitions sont choisies selon des critères d'abondance et de sélectivité, l'une servant à la quantification et l'autre à l'identification [11,26,27,29,30,41,63].…”

Section: Identification Et Quantificationunclassified

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Analyse des cannabinoïdes par spectrométrie de masse en mode tandem

Fabritius¹,

Staub²,

Giroud³

2011

Ann Toxicol Anal

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Résumé -Depuis quelques années, la spectrométrie de masse en tandem (MS/MS) ne cesse de gagner du terrain comme méthode d'analyse en toxicologie forensique, notamment pour le dosage des cannabinoïdes. Couplée à la chromatographie liquide (LC) ou gazeuse (GC), elle permet l'identification fiable et le dosage rapide du THC, de son précurseur acide, et de ses principaux métabolites, y compris les glucuronides. Au cours de ces dix dernières années, un nombre significatif de publications sont parues sur ce sujet. L'objectif de cet article est de passer en revue les analyses par spectrométrie de masse en tandem des cannabinoïdes dans diverses matrices biologiques.Mots clés : Cannabinoïdes, spectrométrie de masse en tandem, matrice biologique, chromatographie Abstract -In recent years, tandem mass spectrometry (MS/MS) is gaining ground as a reference method of analysis in clinical and forensic toxicology, especially for the determination of cannabinoids. Coupled to liquid chromatography (LC) or gas chromatography (GC), it allows the definitive identification and rapid determination of THC, its acid precursor, and its major metabolites, including the glucuronides. During the past decade, several methods of analysis of cannabinoids in different matrices have appeared on this subject. The aim of this paper is to review the analysis of cannabinoids by tandem mass spectrometry methods in various biological matrices.

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Oral Fluid Toxicology

Laloup

Samyn

Gert

2009

Wiley Encyclopedia of Forensic Science

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Technological advances over the past decades have enabled oral fluid testing for the presence of numerous drugs, narcotics, and hormones. The advantages of oral fluid over traditional fluids are that collection is almost noninvasive, is relatively easy to perform, and, in forensic situations, can be achieved under close supervision to prevent adulteration or substitution of the samples. Moreover, concentrations of many drugs in oral fluid correlate well with blood concentrations and, therefore, the analysis of oral fluid specimens can be used to determine the current degree of exposure to a definite drug at the time of sampling. These characteristics have resulted in the use of oral fluid for drugs of abuse testing in the workplace, in testing of intoxicated drivers, for monitoring illicit drug use in drug treatment. There are now sensitive and reliable analytical methods available both for screening and confirmation analysis of oral fluid specimens. External proficiency testing is being developed and cutoff concentrations have been proposed by scientific organizations. Appropriate certification of laboratories worldwide and the introduction of legal provisions in several countries will be the next step. However, a number of concerns require further research. The search for an on‐site screening method that can provide acceptable accuracies for the detection of cannabinoids in oral fluid remains a major hurdle. Additional considerations that have received attention are the variability in the volume of sample collected and the drug recovery from the many different specimen collection systems on the market. This article discusses developments in the field of collection devices, pharmacokinetics of common drugs of abuse in oral fluid, and recent technological advances and guidelines that increase the actual use of oral fluid testing in a legal context and its acceptance by the criminal justice system.

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Detection of THCA in Oral Fluid by GC-MS-MS

Cited by 51 publications

References 11 publications

Nonsmoker Exposure to Secondhand Cannabis Smoke. III. Oral Fluid and Blood Drug Concentrations and Corresponding Subjective Effects

Nonsmoker Exposure to Secondhand Cannabis Smoke. III. Oral Fluid and Blood Drug Concentrations and Corresponding Subjective Effects

Analyse des cannabinoïdes par spectrométrie de masse en mode tandem

Oral Fluid Toxicology

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