Detection of TGIF1 homeobox gene in oral squamous cell carcinoma according to histologic grading

Matizonkas-Antonio, Luciana Fasanella; Libório, Tatiana Nayara; Xavier, Flávia Caló Aquino; Silva‐Valenzuela, Maria das Graças da; Michaluarte-Júnior, Pedro; Nunes, Fábio Daumas

doi:10.1016/j.tripleo.2010.10.003

Cited by 6 publications

(8 citation statements)

References 29 publications

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“…The analysis of the TGIF1 protein showed that there was a statistically significant difference between the nuclear and cytoplasmic expression when comparing OSCC and NT samples. In line with Matizonkas et al (2011), 14 we agree that TGIF1 cytoplasmic immunolocalization implies that some isoforms could develop additional functions other than transcription. Furthermore, Lo et al (2001) 29 revealed that the mitogenactivated protein kinase transducing pathway can phosphorylate TGIF1, prolonging its half-life and consequently raising its protein level, which could also justify the higher levels of TGIF1 protein in the cytoplasmic compartment of OSCC cells.…”

Section: Discussionsupporting

confidence: 92%

“…A previous in situ hybridization study showed that TGIF1 transcripts had a signal that was frequently intense in NT, and generally weak in OSCC, 14 suggesting that TGIF1 expression is higher in NT compared with OSCC. Conversely, by using RT-qPCR, we found no differences regarding TGIF1 expression in OSCC when compared with NT, although an imbalance of specific splicing variants (TGIF1v2, TGIF1v5, and TGIF1v8) was found, which suggests that alternative splicing of TGIF1 may be deregulated in OSCC.…”

Section: Discussionmentioning

confidence: 93%

“…10 Recent publications have described the participation of homeobox genes in OSCC. [11][12][13][14][15] A previous study found that TGIF1 transcripts were expressed differently in OSCC according to histologic grading. 14 TGIF1 belongs to the homeobox family of transcription factors and is alternatively spliced into 8 different splicing variants, encoding 4 distinct protein isoforms (http://ncbi.nlm.nih.gov, Gene ID 7050).…”

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confidence: 99%

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TGIF1 splicing variant 8 is overexpressed in oral squamous cell carcinoma and is related to pathologic and clinical behavior

Libório

Ferreira

Xavier

et al. 2013

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 99%

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TGIF1 splicing variant 8 is overexpressed in oral squamous cell carcinoma and is related to pathologic and clinical behavior

Libório

Ferreira

Xavier

et al. 2013

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

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“…It has been reported that TGIF is involved in at least three signaling pathways, including retinoic acid (RA) ( 6 ), transforming growth factor β (TGF-β) ( 8 ), and wnt/β-catenin signaling pathways ( 9 ). A number of studies have reported that TGIF plays an important role in the initiation, development, and progression of breast cancer ( 9 ), lung cancer ( 10 – 12 ), hepatocellular carcinoma ( 13 , 14 ), upper urinary tract urothelial carcinoma ( 15 , 16 ), leukemia ( 17 – 19 ), and oral squamous cell carcinoma ( 20 , 21 ). Nakakuki et al reported that frequent amplifications of TGIF were observed in esophageal squamous cell carcinoma (ESCC) ( 22 ), which suggests that TGIF might be associated with esophageal tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of TGIF attenuates the proliferation and tumorigenicity of EC109 cells and promotes cisplatin‑induced apoptosis

Wang

Pan

et al. 2017

Oncol Lett

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A previous study has reported that frequent amplifications of the TG-interacting factor (TGIF) were observed in esophageal squamous cell carcinoma. The aim of the present study was to investigate the potential role of TGIF in the proliferation and tumorigenicity of the esophageal cancer cell line EC109 and cisplatin-induced apoptosis. Stable TGIF-knockdown EC109 cell line was established by infecting short hairpin RNA (shRNA) lentiviral particles. Soft agar and tumor xenograft assays were applied in nude mice. Flow cytometry was employed to evaluate the cell cycle and apoptosis. Western blot analysis was used to detect the expression of proteins. TGIF knockdown suppressed EC109 cell proliferation, colony formation in soft agar and tumor growth in nude mice, induced cell cycle arrest in the G1 phase, and promoted cisplatin-induced apoptosis. In addition, TGIF knockdown significantly reduced the expression of phospho-Rb in EC109 cells. The reduced level of full length PARP expression and the increased level of cleaved caspase-3 expression were observed in EC109 cells with the treatment of cisplatin and TGIF knockdown. The results suggest that knockdown of TGIF attenuated the proliferation and tumorigenicity of EC109 cells, and promoted cisplatin-induced apoptosis.

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“…na progressão e metástase do carcinoma epidermóide bucal.A análise de transcritos e proteína do gene homeobox TGIF1 foi recentemente avaliada porMatizonkas et al (2011). Foi demonstrado que este gene encontra-se altamente expresso no tecido oral normal, sendo fracamente expresso em amostras de carcinoma epidermóide bucal.…”

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Análise dos efeitos da superexpressão do gene PROX1 em linhagem celular derivada de carcinoma epidermóide bucal

Rodrigues¹

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Homeobox genes encode transcription factors with an important role during normal development by controlling cellular proliferation and differentiation. Altered expression of PROX1 homeobox gene is related to many cancers, including those of the breast, esophagus, liver, billiary system and lymphomas. The aim of this study was evaluate the effects of PROX1 overexpression, in an oral squamous cell carcinoma cell line, on cellular proliferation and differentiation, apoptosis as well as gene expression prolfile. After overexpression of PROX1 gene in SCC9 cell line, proliferation was assessed by proliferation curve, flow citometry, BrdU incorporation to DNA and Ki67 expression. Cell differentiation was verified by immunocytochemistry to cytokeratins 1, 10, 13, 14, 16, 18 and 19 and apoptosis was measured by annexin V positive cells. Gene expression profile was analyzed by microarray in PROX1-overexpressing cells and control. PROX1-overexpressing cells showed a statistically significant decrease in proliferation as well cytokeratin 1, 13, 18 and 19 expression. No significant differences from controls and PROX1overexpressing cells were observed in apoptosis. Microarray analyses showed differential expression of genes related to development, cellular adhesion an invasion. Our results strongly suggest that overexpression of PROX1 inhibit cell proliferation and contributes to differentiation of oral squamous cell carcinoma.

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Detection of TGIF1 homeobox gene in oral squamous cell carcinoma according to histologic grading

Abstract: TGIF1 gain or loss of function might possibly play a role in oral cancer cell differentiation.

Cited by 6 publications

References 29 publications

TGIF1 splicing variant 8 is overexpressed in oral squamous cell carcinoma and is related to pathologic and clinical behavior

TGIF1 splicing variant 8 is overexpressed in oral squamous cell carcinoma and is related to pathologic and clinical behavior

Knockdown of TGIF attenuates the proliferation and tumorigenicity of EC109 cells and promotes cisplatin‑induced apoptosis

Análise dos efeitos da superexpressão do gene PROX1 em linhagem celular derivada de carcinoma epidermóide bucal

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