Detection of T-2 Toxin in Different Cereals by Flow-Through Enzyme Immunoassay with a Simultaneous Internal Reference

Sibanda, Liberty; Saeger, Sarah De; Peteghem, Carlos Van; Grabarkiewicz-Szczesna, J; Tomczak, Magdalena

doi:10.1021/jf000337k

Cited by 39 publications

(19 citation statements)

References 6 publications

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“…Immunodyne ABC and membrane was coated with 2 microliter of goat anti‐horseradish peroxidase (HRP) and rabbit anti‐mouse (test spot) (undiluted) immunoglobulins, and the free binding sites were blocked. In one study recovery was between 16% and 82% (Sibanda and others 2000). After that, Charoenpornsook and Kavisarasai (2006) developed another method for T‐2 toxin and DON determination, using ELISA on animal feeds.…”

Section: Qualitative and Quantitative Analysismentioning

confidence: 99%

Qualitative and Quantitative Analysis of Mycotoxins

Rahmani¹,

Selamat²,

Soleimany³

2009

Comp Rev Food Sci Food Safe

201

128

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Mycotoxin toxicity occurs at very low concentrations, therefore sensitive and reliable methods for their detection are required. Consequently, sampling and analysis of mycotoxins is of critical importance because failure to achieve a suitable verified analysis can lead to unacceptable consignments being accepted or satisfactory shipments unnecessarily rejected. The general mycotoxin analyses carried out in laboratories are still based on physicochemical methods, which are continually improved. Further research in mycotoxin analysis has been established in such techniques as screening methods with TLC, GC, HPLC, and LC-MS. In some areas of mycotoxin method development, immunoaffinity columns and multifunctional columns are good choices as cleanup methods. They are appropriate to displace conventional liquid-liquid partitioning or column chromatography cleanup. On the other hand, the need for rapid yes/no decisions for exported or imported products has led to a number of new screening methods, mainly, rapid and easy-to-use test kits based on immuno-analytical principles. In view of the fact that analytical methods for detecting mycotoxins have become more prevalent, sensitive, and specific, surveillance of foods for mycotoxin contamination has become more commonplace. Reliability of methods and well-defined performance characteristics are essential for method validation. This article covers some of the latest activities and progress in qualitative and quantitative mycotoxin analysis.

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Section: Qualitative and Quantitative Analysismentioning

confidence: 99%

Qualitative and Quantitative Analysis of Mycotoxins

Rahmani¹,

Selamat²,

Soleimany³

2009

Comp Rev Food Sci Food Safe

201

128

View full text Add to dashboard Cite

show abstract

“…The dilution of the antibodies specific to the enzyme was chosen so that the colour intensity of the control zone was nearly the same as the colour intensity of the test zone in the case of a negative result. This approach was applied to the determination of T-2 in wheat, maize, oat and rye (Sibanda et al, 2000), OTA in roasted and green coffee beans (Sibanda et al, 2001), and fumonisins in maize (Paepens et al, 2004). In these cases, the LOD was determined based on complete colour suppression.…”

Section: Principles Applications and Challenges In Mycotoxin Analysismentioning

confidence: 99%

Immunochemical methods for rapid mycotoxin detection in food and feed

Goryacheva

Saeger

2011

Determining Mycotoxins and Mycotoxigenic Fungi in Food and Feed

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“…Therefore, immunoassay methods for fumonisins analysis using polyclonal and monoclonal antibodies have been developed in the past two decades because of their, simplicity and selectivity [11]. Hence, on-site immunochemical techniques such as dipstick [15] immunochromatography [16], immunofiltartion [17], enzyme–linked immunosorbant assays (ELISA) and immunosensor techniques [18,19] are gaining interest for mycotoxin detection. …”

Section: Introductionmentioning

confidence: 99%

Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods

Kadir

Tothill

2010

Toxins

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An electrochemical affinity sensor for the determination of fumonisins mycotoxins (Fms) using monoclonal antibody modified screen-printed gold electrode with carbon counter and silver-silver chloride pseudo-reference electrode is reported in this work. A direct competitive enzyme-linked immunosorbent assay (ELISA) was initially developed, exhibiting a detection limit of 100 µg·L-1 for fumonisins. This was then transferred to the surface of a bare gold screen-printed electrode (SPGE) and detection was performed by chronoamperometry, monitoring the reaction of 3,3’,5,5’-Tetramethylbenzidine dihydrochloride (TMB) and hydrogen peroxide (H2O2) catalysed by HRP at −100 mV potential vs. onboard Ag-AgCl pseudo-reference electrode. The immunosensor exhibited detection limit of 5 µg·L−1 fumonisins with a dynamic range from 1 µg·L−1–1000 µg·L−1. The sensor also performed well in extracted corn samples.

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Detection of T-2 Toxin in Different Cereals by Flow-Through Enzyme Immunoassay with a Simultaneous Internal Reference

Cited by 39 publications

References 6 publications

Qualitative and Quantitative Analysis of Mycotoxins

Qualitative and Quantitative Analysis of Mycotoxins

Immunochemical methods for rapid mycotoxin detection in food and feed

Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods

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