Detection of synergistic hemolytic activity of Staphylococcus aureus with the Cathra replicator

Boyce, J M

doi:10.1128/jcm.21.5.835-837.1985

Cited by 3 publications

(1 citation statement)

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“…Furthermore, hlg is the gene encoding gamma hemolysin in 99.5% of S. aureus isolates (Prevost, 1995), whereas the current study found hlg in 98 (88.28%) of all 111 isolates, which included in twenty-four bacterial species, followed by hlb in 75 (67.57%) and hla in 46 (41.4%) because the platelets and monocytes are more sensitive to alpha hemolysis (Todar, 2005). Moreover, the expression of beta hemolysis on BA is influenced by the concentration of magnesium and the chelating agent in the prepared medium (Boyce, 1985). As a result to the inhibitory effect of agar on gamma hemolysis activity, the rabbit blood agarose was used to detect their activity (Divyakolu et al, 2019).…”

Section: Most Infectious Diseases Were Caused Bymentioning

confidence: 99%

Distribution of hla, hlb, hlgC, hld & cylA hemolysin genes and their alleles in different bacterial species isolated from the variant clinical sources in Basrah

2023

JPTCP

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Hemolysin is the most important virulence factor responsible for defecting in the plasma membranes of various types of host cells. One hundred and twenty-eight samples were collected from different clinical sources showing 111 (86.72%) bacterial isolates including 24 different bacterial species identified by 16S rRNA sequencing. Most of bacterial isolates have the ability to lyse the blood cells, delta hemolysin showed 50 (45.05 %) of isolates were positive on blood agar (BA), while 97 (87.39%) of isolates had the hld gene, followed by gamma 47(42.43%) on BA and 98 (88.28%) have of hlgC and 39 (35.14%) of isolates as beta on BA and 75 (67.57%) of hlb followed by alpha 37(33.33%) on BA and 46 (41.4%) of hla. Finally, 68 (61.26%) had the cytolysin A gene (cylA). The sequence of hemolysins revealed different number of alleles for hla, hlb, hlg and hld except for the cylA gene containing one allele only. The hemolysins were detected in 24 different bacterial species in addition to S. aureus. hla showed a new spontaneous mutation containing a new allele recorded in the NCBI.

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Section: Most Infectious Diseases Were Caused Bymentioning

confidence: 99%

Distribution of hla, hlb, hlgC, hld & cylA hemolysin genes and their alleles in different bacterial species isolated from the variant clinical sources in Basrah

2023

JPTCP

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Hemolytic Activity of Staphylococcus hyicus and Staphylococcus chromogenes

Skalka¹

1988

Acta Vet. Brno

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S k a 1 k a B.: Hemolytic activity of Staphylococcus hyicus and Staphylococcus chromogenes. Acta vet. Bmo, 57, 1988: 3-11. Hemolytic activity of 135 ~ hyicus and 75 ~ chromogenes strains was examined on sheep, rabbit, human, horse and chicken blood agars. The agar bases were supplemented with 5 % washed erythrocytes of the corresponding animal species. The strains of both staphylococcal species under study produced a wide zone of complete hemolysis on rabbit blood agar and a narrow one on media containing h9~se~rythrocytes. ~e hemolytic effects always appeared after 48 h incubation at 37 C. Neither species produced hemolysis on agar containing sheep or chicken erythrocytes, and no synergistic hemolytic effect was observed on sheep blood agar. A direct hemolysis was exhibited by 92 (68 %) ~ hyicus strains on human blood agars. The remaining ~ hyicus strains and ~ chromo genes did not produce hemolysis of human erythrocytes. Hemolytic effects of the staphylococci used as controls were expressed just after 24 h incubation on all kinds of blood agar. We recOllllleJld inclusion of the hemolysis of rabbit erythrocytes among the characteristic properties of ~ hyicus and ~ chromogenes.Staphylococcus hyicus, Staphylococcus chromogenes, hemolytic activity, blood agar, sheep, rabbit, huI:Dan, horse and chicken e~cytes.

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Antagonistic Effect of Staphylococcus hyicus and Staphylococcus chromogenes Exhibited on Staphylococcal Delta Hemolysin

Skalka¹

1991

Acta Vet. Brno

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. Skalka B.: Antagonistic Effect of Staphylococcus hyicus and Staphylococcus chromogenes Exhibited on Staphylococcal DeltaHemolysin. Acta vet. Bmo, 60,1991: 61-69. Staphylococcus hyicus and Staphylococcus chromo genes are able to inhibit hemolytic effect of the staphylococcal delta hemolysin on agar media supplemented with human or sheep erythrocytes. This inhibition takes place even when the effect of delta hemolysin on agars with sheep erythrocytes is potentiated with staphylococcal beta hemolysin. Although less markedly expressed, similar antagonistic effect on staphylococcal delta hemolysin is also found in' a majority of strains Staphylococcus sciuri, Staphylococcus lentus and some Micrococcus spp. Other species of coagulase-negative staphylococci have no antagonistic effect on delta hemolysin. A technique of a combined test of hemolytic interactions (CTHI) for a simultaneous assay of hemolytic antagonism and synergism was proposed, which can be used to differentiate S. hyicus and S. chromogenes from other novobiocin-sensitive staphylococcal species. Staphylococcus hyicus, Staphylococcus chromo genes, staphylococcal delta hemolysin, antagonistic effect, combined test of hemolytic interactions (CTHI)In our previous paper (Skalka 1988) we described hemolytic activity of S. hyicus and S. chro-:mogenes on blood agars with rabbit or human erythrocytes. Hemolytic effects of these two staphylococcal species on agar media supplemented with erythrocytes of various animal origin differed from the data on sensitivity of various erythrocytes to staphylococcal hemolysins alpha, beta, gammaand delta (Dolman 1932;Glenny and Stevens 1935;Smith and Price 1938;Williams and Harper 1947;Marks and Vaughan 1950;Jackson 1962;Jeljaszewicz 1972;Wiseman 1975). In the paper mentioned we were not even able to confirm the descriptions of delta hemolysin production by certain strains of the two staphylococcal species (Hebert and Hancock 1985;Lammler and Blobel 1987;Watts and Owens 1987) or the information on their ability to pro·duce gamma hemolysin (Goodfellow et al. 1987).The discrepancy between our findings and the descriptions of other authors cited stimulated our continuous study of hemolytic manifestations of the two above-mentioned staphylococcal :1lpecies, which is the subject-matter of the present paper. Materials and Methods Blood agarsBrain heart infusion agar CM 375 (Oxoid Ltd.) or "basis for blood agar No.4" (Imuna) were supplemented with 5 % (v/v) washed sheep or human erythrocytes.Tested Strains .The set of tested strains of staphylococci included 247

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Detection of synergistic hemolytic activity of Staphylococcus aureus with the Cathra replicator

Cited by 3 publications

References 10 publications

Distribution of hla, hlb, hlgC, hld & cylA hemolysin genes and their alleles in different bacterial species isolated from the variant clinical sources in Basrah

Distribution of hla, hlb, hlgC, hld & cylA hemolysin genes and their alleles in different bacterial species isolated from the variant clinical sources in Basrah

Hemolytic Activity of Staphylococcus hyicus and Staphylococcus chromogenes

Antagonistic Effect of Staphylococcus hyicus and Staphylococcus chromogenes Exhibited on Staphylococcal Delta Hemolysin

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