Detection of Superoxide Alterations Induced by 5-Fluorouracil on HeLa Cells with a Cell-Based Biosensor

Mavrikou, Sophie; Tsekouras, Vasileios; Karageorgou, Maria-Argyro; Moschopoulou, Georgia; Kintzios, Spyridon

doi:10.3390/bios9040126

Cited by 13 publications

(9 citation statements)

References 66 publications

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“…Among these drugs, docetaxel significantly increased the expression level of caspase-3 within 8 h, which is consistent as reported previously that docetaxel exhibited strong apoptotic induction effect both on HeLa cells and MCF-7 cells (Al-Abd et al, 2011). 5-FU and adriamycin induced the activation of caspase-3 at 48 h in Hela cells, which is similar to the former studies (Brisdelli et al, 2016;Mavrikou et al, 2019). In contrast, the induction of caspase-3 activity in MCF-7 cells by adriamycin was negligible relative to that in HeLa cells, while caspase-3 in MCF-7 cells was not activated by 5-FU stimulation.…”

Section: Discussionsupporting

confidence: 92%

A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells

Gong

Wang

Abbas

et al. 2021

Sci. China Life Sci.

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Apoptosis is a form of programmed cell death that is essential for maintaining internal environmental stability. Disordered apoptosis can cause a variety of diseases; therefore, sensing apoptosis can provide help in study of mechanism of the relevant diseases and drug development. It is known that caspase-3 is a key enzyme involved in apoptosis and the expression of its activity is an indication of apoptosis. Here, we present a genetically encoded switch-on mNeonGreen2-based molecular biosensor. mNeonGreen2 is the brightest monomeric green fluorescent protein. The substrate of caspase-3, DEVD amino acid residues, is inserted in it, while cyclized by insertion of Nostoc punctiforme DnaE intein to abolish the fluorescence (inactive state). Caspase-3-catalyzed cleavage of DEVD linearizes mNeonGreen2 and rebuilds the natural barrel structure to restore the fluorescence (activated state). The characterization exhibited that the Caspase-3 biosensor has shortened response time, higher sensitivity, and prolonged functional shelf life in detection of caspase-3 amongst the existing counterparts. We also used the Caspase-3 biosensor to evaluate the effect of several drugs on the induction of apoptosis of HeLa and MCF-7 tumor cells and inhibition of Zika virus invasion. Supporting Information The supporting information is available online at 10.1007/s11427-021-1986-7. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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Section: Discussionsupporting

confidence: 92%

A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells

Gong

Wang

Abbas

et al. 2021

Sci. China Life Sci.

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“…This result confirmed our anticipation of the higher level of ROS in cancer cells and tissues compared to healthy ones (25,26). Also, various groups utilized SOD-based biosensors for detecting superoxide radicals in cancer cells (27) and tissues (13,28), and reported higher levels of superoxide radicals in the cancer samples. For instance, Campanella et al (2000) has reported an approximately 15 times more intense amperometric response for cancerous renal tissue compared to healthy tissue (13).…”

Section: Clinical Application Of Gelatin-alginate-tio2-sod Biosensorsupporting

confidence: 85%

Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals

Karakaya

Derkuş

Emregül

2020

Journal of the Turkish Chemical Society Section A: Chemistry

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In this work, a biosensor that uses gelatin and alginate hydrogels in addition to titanium dioxide (TiO2) nanoparticles (NPs) as a sensor matrix was developed to detect superoxide radicals (O2 •-), which play a role in carcinogenesis when present in excess levels. Parameters affecting the performance of the biosensor such as the amount of gelatin-alginate ratio, amount of TiO 2 NPs, the concentration of SOD enzymes, and glutaraldehyde (GA) cross-linker were investigated. Chronoamperometry was used as an electrochemical technique for the development of biosensor as well as characterization steps. The developed biosensor exhibited two linear ranges between 0.0009 mM -0.125 mM and 0.25 mM -2 mM, which was utilized as calibration curves. The detection limit of the biosensor was found 0.9 μM, which was at the appropriate level for the detection of O2 •-in tumor samples. Finally, the constructed biosensor showed significant analytical performance, such as low detection limit, reusability, and reproducibility.

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“…Viabilities lower than 20% in cancer cells were found for both systems at 72 h, at the highest and lowest dilutions. This result is consistent with previous findings where low concentrations of 5-FU induced cytotoxicity on HeLa cells 49.…”

supporting

confidence: 94%

Chondroitin/polypyrrole nanocomposite hydrogels for the accurate release of 5‐fluorouracil by electrical stimulation

Bustamante

Salas

Castillo‐Ortega

et al. 2022

Polymers for Advanced Techs

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The development of electro‐stimulated drug release devices is an innovative approach to attain the drug delivery in accurate doses at target sites in a programmed manner. In this work, novel electroactive nanocomposite hydrogels were prepared by encapsulating green‐synthesized polypyrrole (PPy) colloids within chondroitin sulfate (CS) networks during the self‐crosslinking of CS via N‐ethyl‐N′‐(3‐dimethylaminopropyl) carbodiimide chemistry. The structural and morphological properties of CS/PPy hydrogels were studied by Fourier‐transformed infrared spectroscopy, scanning electron microscopy, and swelling kinetic measurements. The chemotherapeutic agent 5‐fluorouracil (5‐FU) was loaded into CS/PPy samples by hydrogel swelling method, or alternatively, by pre‐incubating the drug in polymer mixture before crosslinking. Different electrical stimulations can be used to switch ON and accurately tune the 5‐FU delivery from GG/PPy hydrogels. A single pulse potential of 5 V switched on the drug delivery up to 90% from nanocomposite hydrogel, in contrast to the low 5‐FU amount released in a passive form (< 20%). PPy electroactive behavior played a determining role as the main driving force in 5‐FU release activation. Cytotoxicity of hydrogels with and without 5‐FU was examined in normal and cancer cells. Considering the high cytotoxicity of 5‐FU, the ON/OFF 5‐FU release patterns evidenced the potential of CS/PPy hydrogels for electrically controlled drug delivery in implantable or transdermal drug release devices.

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Detection of Superoxide Alterations Induced by 5-Fluorouracil on HeLa Cells with a Cell-Based Biosensor

Cited by 13 publications

References 66 publications

A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells

A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells

Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals

Chondroitin/polypyrrole nanocomposite hydrogels for the accurate release of 5‐fluorouracil by electrical stimulation

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