Detection of superantigenic toxin genes in Staphylococcus aureus strains from subclinical bovine mastitis

Gunaydin, Besime; Aslantaş, Özkan; Demir, Cemil

doi:10.1007/s11250-011-9882-5

Cited by 20 publications

(23 citation statements)

References 18 publications

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“…A high incidence of seg-sei genes was also found. It had been previously reported that the sei gene was common in isolates from subclinical bovine mastitis (15) and retail meats (36). In this study, this gene was also present in all strains from the seafood industry.…”

Section: Discussionsupporting

confidence: 69%

Incidence of Staphylococcus aureus and Analysis of Associated Bacterial Communities on Food Industry Surfaces

Gutiérrez

Delgado

Vázquez‐Sánchez

et al. 2012

Appl Environ Microbiol

193

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bBiofilms are a common cause of food contamination with undesirable bacteria, such as pathogenic bacteria. Staphylococcus aureus is one of the major bacteria causing food-borne diseases in humans. A study designed to determine the presence of S. aureus on food contact surfaces in dairy, meat, and seafood environments and to identify coexisting microbiota has therefore been carried out. A total of 442 samples were collected, and the presence of S. aureus was confirmed in 6.1% of samples. Sixty-three S. aureus isolates were recovered and typed by random amplification of polymorphic DNA (RAPD). Profiles were clustered into four groups which were related to specific food environments. All isolates harbored some potential virulence factors such as enterotoxin production genes, biofilm formation-associated genes, antibiotic resistance, or lysogeny. PCR-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprints of bacterial communities coexisting with S. aureus revealed the presence of bacteria either involved in food spoilage or of concern for food safety in all food environments. Food industry surfaces could thus be a reservoir for S. aureus forming complex communities with undesirable bacteria in multispecies biofilms. Uneven microbiological conditions were found in each food sector, which indicates the need to improve hygienic conditions in food processing facilities, particularly the removal of bacterial biofilms, to enhance the safety of food products.

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Section: Discussionsupporting

confidence: 69%

Incidence of Staphylococcus aureus and Analysis of Associated Bacterial Communities on Food Industry Surfaces

Gutiérrez

Delgado

Vázquez‐Sánchez

et al. 2012

Appl Environ Microbiol

193

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“…Nineteen of 102 S. aureus isolates (18.6%) recovered from BTM and milk filters from national milk-producing herds in Ireland showed the presence of one or more enterotoxin genes (44). Conversely, a higher percentage of isolates, 46.9% of 130, associated with subclinical bovine mastitis in Turkey showed the presence of one or more enterotoxin genes (23). The aim of the present study was to characterize and document the prevalence and distribution of MSSA and MRSA in bulk tank milk samples.…”

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confidence: 99%

Prevalence and Characterization of Staphylococcus aureus, Including Methicillin-Resistant Staphylococcus aureus, Isolated from Bulk Tank Milk from Minnesota Dairy Farms

et al. 2012

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Staphylococcus aureus is a common causative agent of bovine mastitis in dairy herds. The emergence of methicillin-resistant Staphylococcus aureus (MRSA) in hospitals as well as the community is a significant and costly public health concern. S. aureusrelated bovine mastitis is a common reason for therapeutic and/or prophylactic use of antibiotics on dairy farms. In this study, herd prevalence of S. aureus, including MRSA, was estimated from bulk tank milk (BTM) from Minnesota farms. A total of 150 pooled BTM samples from 50 farms, collected over 3 seasons (spring, summer, and fall of 2009), were assessed. Herd prevalence of methicillin-susceptible S. aureus (MSSA) was 84%, while MRSA herd prevalence was 4%. A total of 93 MSSA isolates and 2 MRSA isolates were recovered from 150 BTM samples. Antibiotic susceptibility testing of S. aureus isolates showed pansusceptibility in 54 isolates, resistance to a single antibiotic class in 21 isolates, resistance to two antibiotic classes in 13 isolates, and resistance to >3 antibiotics classes and thus multidrug resistance in 5 isolates. The two MRSA isolates displayed resistance to ␤-lactams, cephalosporins, and lincosamides and were multiresistant. Staphylococcal protein A gene (spa) typing identified spa types t529 and t034 most frequently among methicillin-susceptible isolates, while t121 was observed in MRSA isolates. Seven isolates, including the two MRSA isolates, produced staphylococcal enterotoxins B, C, D, and E on overnight culture. MRSA isolates were further genotyped using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Of the 2 MRSA isolates, one had a composite genotype profile of MLST ST 5-PFGE USA100-unknown spa type, which has been reported among hospital-associated MRSA isolates, while the second isolate carried the MLST ST 8-PFGE USA300-spa type t121 genotype, commonly identified among community-associated MRSA isolates. These results suggest that MRSA genotypes associated with hospitals and community can be isolated from milk at very low rates.

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“…Nos estudos realizados no nordeste tão pouco foram encontrados isolados de S. aureus portadores deste gene (Almeida et al 2013, Ferreira et al 2014, Silveira-Filho et al 2014. Em outros paí-ses também são descritos estudos onde a frequência deste gene é cero nos isolados de S. aureus circulantes, por exemplo na Itália (Perillo et al 2012, Ruaro et al 2013, Turquia (Günaydın et al 2011) e no Estado da Califórnia (USA) (Cenci-Goga et al 2003). A toxina SEB é considerada uma arma biológica e sua inalação pode provocar lesões pulmonares e insuficiência respiratória (Rao et al 2014).…”

Section: Discussionunclassified

“…No Estado da Paraí-ba, o gene que codifica para essa toxina não foi detectado (Ferreira et al 2014). Internacionalmente também não foi encontrado o gene seh em isolados de S. aureus causadores de mastite bovina na Turquia (Günaydın et al 2011) em leite de ovelhas na Itália (Perillo et al 2012) ou são relatadas frequência baixa (1,7%) na Bélgica (Ote et al 2011).…”

Section: Discussionunclassified

Frequência de genes codificadores de toxinas em Staphylococcus aureus isolados de leite de tanques expansão comunitários

et al. 2017

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RESUMO.-A capacidade de produção de toxinas pelo Staphylococcus aureus no leite e produtos derivados está relacionado com surtos de intoxicação alimentar. Objetivou-se nesta pesquisa, estudar a ocorrência de genes que codificam para enterotoxinas estafilocócicas (sea, seb, sed, seg, seh e sei) e toxinas α e β hemolítica (hla e hlb) em S. aureus isolados de 53 amostras de leite de tanques expansão comunitários no Estado de Alagoas, Brasil. Foram identificados 27 isolados (50,94%) como S. aureus pela amplificação do gene nuc. 13/27 isolados (48,1%) foram positivos para pelo menos um gene das enterotoxinas estudadas, sendo as frequências dos genes sea 33,3%, seh 18,5%, sei 11,1% e sed 7,4%; não entanto não foram identificados os genes seb e seg nestas bactérias. Para as toxinas hemolíticas, 51,9% dos isolados portavam ambos genes (hla e hlb), sendo a frequência para o gene hla de 81,5% e para o gene hlb de 51,9%. A frequência de genes das toxinas avaliadas é alta o que constitui um risco potencial para a saúde pública em especial, as enterotoxinas por serem termoestáveis e estarem asssociados com surtos de intoxicação alimentar. The capacity of toxin production by Staphylococcus aureus in milk and dairy products is associated with food poisoning outbreaks. The objective of this research was to study the frequency of genes encoding staphylococcal enterotoxin (sea, seb, sed, seg, seh and sei) and α and β hemolytic toxins (hla and hlb) in S. aureus isolates from 53 milk samples from community tanks in the State of Alagoas, Brazil. Twenty-seven isolates (50.94%) were identified as S. aureus by nuc gene amplification; 13/27 isolates (48.1%) were positive for at least one gene of the studied enterotoxins and the frequency of genes sea was 33.3%, seh 18.5%, sei 11.1% and sed 7.4%; the seb and sec genes have not been identified in the bacteria. For the hemolytic toxins, 51.9% of isolates harbored both genes (hla and hlb), the frequency of hla gene was 81.5% and 51.9% for the hlb gene. The evaluated toxin-encoding gene frequency is high and constitutes a potential risk for public health, especially staphylococcal enterotoxin genes; because they are heat-stable enterotoxins and have been associated with food poisoning.

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Detection of superantigenic toxin genes in Staphylococcus aureus strains from subclinical bovine mastitis

Cited by 20 publications

References 18 publications

Incidence of Staphylococcus aureus and Analysis of Associated Bacterial Communities on Food Industry Surfaces

Incidence of Staphylococcus aureus and Analysis of Associated Bacterial Communities on Food Industry Surfaces

Prevalence and Characterization of Staphylococcus aureus, Including Methicillin-Resistant Staphylococcus aureus, Isolated from Bulk Tank Milk from Minnesota Dairy Farms

Frequência de genes codificadores de toxinas em Staphylococcus aureus isolados de leite de tanques expansão comunitários

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