Detection of subtle cartilage and bone tissue degeneration in the equine joint using polarisation-sensitive optical coherence tomography

Goodwin, Matthew; Klufts, Marie; Workman, Joshua; Thambyah, Ashvin; Vanholsbeeck, Frédérique

doi:10.1016/j.joca.2022.04.006

Cited by 2 publications

(3 citation statements)

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“…During the last decade, optical imaging and spectroscopic modalities including optical coherent tomography, second harmonic generation microscopy, and Raman spectroscopy have been employed to evaluate degenerative changes in the cartilage matrix of murine models or human specimens 6 – 16 In particular, Raman spectroscopy is a nondestructive analytical tool, which provides information about the molecular structure, polarization, crystallinity, and conformation. The cartilage matrix mainly consists of Type II collagen and proteoglycans (PGs) underlying a collagen fibril meshwork 17 .…”

Section: Introductionmentioning

confidence: 99%

Prognostic potential and pathological validation of a diagnostic application using Raman spectroscopy in the characterization of degenerative changes in the cartilage of the humeral head

et al. 2022

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Significance: Raman spectroscopy is a well-established analytical method in the fields of chemistry, industry, biology, pharmaceutics, and medicine. Previous studies have investigated optical imaging and Raman spectroscopy for osteoarthritis (OA) diagnosis in weight-bearing joints such as hip and knee joints. However, to realize early diagnosis or a curable treatment, it is still challenging to understand the correlations with intrinsic factors or patients' background.Aim: To elucidate the correlation between the Raman spectral features and pathological variations of human shoulder joint cartilage.Approach: Osteoarthritic cartilage specimens excised from the humeral heads of 14 patients who underwent shoulder arthroplasty were assessed by a confocal Raman microscope and histological staining. The Raman spectroscopic dataset of degenerative cartilage was further analyzed by principal component analysis and hierarchical cluster analysis.Results: Multivariate association of the Raman spectral data generated three major clusters. The first cluster of patients shows a relatively high Raman intensity of collagen. The second cluster displays relatively low Raman intensities of proteoglycans (PGs) and glycosaminoglycans (GAGs), whereas the third cluster shows relatively high Raman intensities of PGs and GAGs. The reduced PGs and GAGs are typical changes in OA cartilage, which have been confirmed by safranin-O staining. In contrast, the increased Raman intensities of collagen, PGs, and GAGs may reflect the instability of the cartilage matrix structure in OA patients. Conclusions:The results obtained confirm the correlation between the Raman spectral features and pathological variations of human shoulder joint cartilage. Unsupervised machine learning methods successfully yielded a clinically meaningful classification between the shoulder OA patients. This approach not only has potential to confirm severity of cartilage defects but also to determine the origin of an individual's OA by evaluating the cartilage quality.

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Section: Introductionmentioning

confidence: 99%

Prognostic potential and pathological validation of a diagnostic application using Raman spectroscopy in the characterization of degenerative changes in the cartilage of the humeral head

et al. 2022

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“… 6 It has been shown to be feasible for articular cartilage assessment in OA. 2 , 7 , 8 In 2010, Chu et al. 2 demonstrated that OCT may become a potential tool to complement arthroscopy for clinical use by providing quantifiable, microscopic-resolution cross-sectional information on cartilage.…”

mentioning

confidence: 99%

“…Further study showed that combining polarization controllers can render OCT sensitive to birefringence detection and improve OCT for early OA assessment. 7 , 9 However, OCT has its limitations, including heavy dependence on operator-use and image postprocessing.…”

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confidence: 99%

Ultraviolet-Visible-Near Infrared Spectroscopy May Aid in the Qualitative Assessment of Early-Stage Cartilage Degradation

Chen,

Huang

2024

Arthroscopy, Sports Medicine, and Rehabilitation

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Detection of subtle cartilage and bone tissue degeneration in the equine joint using polarisation-sensitive optical coherence tomography

Cited by 2 publications

References 41 publications

Prognostic potential and pathological validation of a diagnostic application using Raman spectroscopy in the characterization of degenerative changes in the cartilage of the humeral head

Prognostic potential and pathological validation of a diagnostic application using Raman spectroscopy in the characterization of degenerative changes in the cartilage of the humeral head

Ultraviolet-Visible-Near Infrared Spectroscopy May Aid in the Qualitative Assessment of Early-Stage Cartilage Degradation

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