Detection of Streptococcus Species by Polymerase Chain Reaction in Infectious Crystalline Keratopathy

Osakabe, Yasuhiro; Yaguchi, Chiemi; Miyai, Takashi; Mineo, Shoichiro; Nakamura, Masaki; Amano, Shiro

doi:10.1097/01.ico.0000240083.31432.70

Cited by 15 publications

(9 citation statements)

References 14 publications

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“…Multiplex PCR was performed on 100 samples using primers (Metabion, Germany) targeting the streptokinase precursor, see I and sod A genes as previously described (Alber et al., 2004; Preziuso & Cuteri, 2012; Preziuso et al., 2010), and 16S rRNA was employed as an internal control gene (Osakabe et al., 2006; Table S2). The amplification reaction contained 25 µl of Emerald Amp® GT PCR master mix (Cat.…”

Section: Methodsmentioning

confidence: 99%

Novel Streptococcus equi strains causing strangles outbreaks in Arabian horses in Egypt

Tartor

El-Naenaeey

Gharieb³

et al. 2020

Transbound. Emerg. Dis.

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Strangles displays a major challenge to veterinary medicine worldwide. However, no data on Streptococcus equi subsp. equi (S. equi) M protein alleles have been reported so far from Arabian horses. We report here for the first time the S. equi SeM alleles causing strangles in Arabian horses, and the associated risk factors for the disease. Duplicate samples from one hundred Arabian horses with acute strangles in confirmed outbreaks and sporadic cases were analysed by phenotypic methods and multiplex polymerase chain reaction (PCR) targeting streptokinase precursor, seeI and sodA genes. PCR and sequencing of S. equi SeM gene were employed for strains typing, and the four superantigens were determined among the allelic variants. Directsample PCR confirmed and highly positively correlated (r = .85) with the phenotypic results, and detected S. equi in five samples more than the conventional culture. A combination of multiplex PCR from samples and culture could successfully identify S. equi (92%), S. zooepidemicus (5%) and S. equisimilis (3%). SeM typing demonstrated five SeM alleles, including four previously unidentified alleles that were deposited in the PubMLST-SeM database. SeM-139 and SeM-141 are related to some strains that were recently recovered from donkeys in China. SeM-140 and SeM-199 are related to a group of alleles from horses in Europe. Variation in the presence of seeM, seeH and seeL superantigens was found across the four novel alleles without interference with the severity of strangles and clinical presentation seen in different outbreaks. Horse age was the most important factor in developing strangles, followed by seasonality and the diagnosis of strangles in the previous year. These new findings comprise a significant contribution to the horse industry through the identification of novel S. equi SeM types that may bolster measures for strangles control as the identified SeM alleles will certainly help in the development of SeM-containing vaccine.

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Section: Methodsmentioning

confidence: 99%

Novel Streptococcus equi strains causing strangles outbreaks in Arabian horses in Egypt

Tartor

El-Naenaeey

Gharieb³

et al. 2020

Transbound. Emerg. Dis.

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“…9 S. viridans is the most common causative organism in ICK. Others include Gemella haemolysans, 6 Serratia marescens, 7 Borrelia garinii, 8 Candida sp., 2,10 Streptococcus pneumoniae, 10,11 Staphylococcus epidermidis, 11 Acanthamoeba sp., 11 Haemophilus sp., 11 Mycobacterium sp., and 11 peptostreptococcus sp. 12 We present the first case of a patient with A. defectivaassociated crystalline keratopathy, confirmed by microbiologic cultures and PCR.…”

Section: Discussionmentioning

confidence: 99%

Infectious Crystalline Keratopathy Caused by Streptococcus Abiotrophia defectiva

Abry

Sauer

Riegel

et al. 2010

Cornea

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“… 4 , 5 Streptococcus viridans is the most common organism to cause crystal deposits followed by Staphylococcus epidermidis, Streptococcus pneumonia, Haemophilus spp., and enterococci. 15 However, Candida spp. and atypical organisms such as mycobacteria must be kept in mind, especially in immune-suppressed conditions such as patients who use chronic corticosteroids or abuse topical anesthetic eye drops.…”

Section: Discussionmentioning

confidence: 99%

Unusual Course of Crystalline Keratopathy in a Patient with Graft-Versus-Host Disease

Bostanci

2017

tjo

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We present a case of infectious crystalline keratopathy in a patient with Graft-versus-Host disease (GVHD) who developed satellite fungal keratitis. A 51-year-old man was referred for bilateral total persistent corneal epithelial defects with severe dry eye. Although persistent epithelial defect healed with medical therapy, he developed stromal keratitis with satellite lesions confirmed to be secondary to Candida albicans. After three months of antifungal treatment and debridement, improvement of the infiltrates was obtained. Crystalline keratopathy is an important clinical entity which may develop due to several causes. The microbial causes include not only bacteria but fungi as well. Careful investigation must be performed, especially for immune-compromised patients, in order to provide appropriate and timely treatment.

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Detection of Streptococcus Species by Polymerase Chain Reaction in Infectious Crystalline Keratopathy

Abstract: Our findings suggest that PCR provides a good adjunct technique in the diagnosis of corneal infection.

Cited by 15 publications

References 14 publications

Novel Streptococcus equi strains causing strangles outbreaks in Arabian horses in Egypt

Novel Streptococcus equi strains causing strangles outbreaks in Arabian horses in Egypt

Infectious Crystalline Keratopathy Caused by Streptococcus Abiotrophia defectiva

Unusual Course of Crystalline Keratopathy in a Patient with Graft-Versus-Host Disease

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