“…At present, PCR is used in the detection of different infectious agents, e.g. S. aureus (1,11), S. pneumoniae (6,19), Mycobacterium (7,18), Chlamydia trachomatis (3), Pseudomonas (2, 10), etc. Many published protocols utilize different modifications of a direct PCR ("nested," "multiplex," and the like) and can detect 103-104 bacteria during 4-5 hr.…”