Detection of strain-specific serological activity in antisera of tobacco mosaic virus, clover yellow mosaic virus, and potato virus X, by complement fixation

Characterization of Monoclonal Antibodies against Potato Virus X and Comparison of Serotypes with Resistance Groups

“…Torrance, unpublished results). In cross-absorption experiments, however, Bawden & Sheffield (1944), Matthews (1949) and Wright (1963) were able to distinguish PVX strains serologically. This paper describes the production and characterization of monoclonal antibodies (MAbs) to representatives of two strains of PVX: DX (Jones, 1982), which belongs to strain group 3 in Cockerham's classification, the commonest of the strain groups; and HB, so far found only in South America, which overcomes the effect of all three known genes conferring resistance to PVX (Nx, Nb and Rx) in cultivated potato (Moreira et al, 1980).…”

“…Strains are classified into four groups on the basis of their reactions with the genes Nx and Nb which, in cultivated varieties of potato, determine hypersensitive responses to PVX infection (Cockerham, 1955(Cockerham, , 1970. Studies using antisera against PVX in assays, including the direct ELISA and the latex agglutination test, have shown that different strains of PVX cannot be readily distinguished with such reagents : PVX strains do not differ by serological differentiation indices of more than two or three units (Wright, 1963 ;Fribourg, 1975;Moreira et al, 1980;L. Torrance, unpublished results).…”

New Approaches to the Detection of Microbial Plant Pathogens

“…These assays could be divided into three types: dilution endpoint techniques, agar diffusion techniques and electrophoresis techniques. The dilution endpoint techniques include the tube precipitin test (Hitchborn and Hills, 1968), the microprecipitin test (van Slogteren, 1955), the ring interface test (Whitcomb and Black, 1961), the flocculation or adsorption precipitin test (Bozicevich, Scott and Vincent, 1963;Cunningham, Tinsley and Walker, 1966;de Sequeira and Lister, 1969), latex agglutination, the quantitative precipitin test (Matthews, 1967) and the complement fixation test (Wright, 1963;Tremaine and Wright, 1967). Agar diffusion techniques include the single-(Shepard, Hardham and is based on the results of Hardham.…”

PVX MS, a New Strain of Potato Virus that Overcomes the Extreme Resistance Gene Rx

“…No serological differences were found between various PVX strains using polyclona! antibodies in either ELISA or latex agglutination tests (WRIGHT 1963, FRIBOURG 1975, MOREIRA et al 1980. ToRRANCE et al (1986) produced monoclonal antibodies whicb are able to distinguish between PVX strains.…”