Detection of starch granules in a living plant by optical second harmonic microscopy

Mizutani, Goro; Sonoda, Yusuke; Sano, Hikaru; Sakamoto, Masaru; Takahashi, Takéo; Ushioda, S.

doi:10.1016/s0022-2313(99)00428-7

Cited by 65 publications

(57 citation statements)

References 8 publications

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“…In the field of microscopy, intrinsic SHG is known to provide structural contrasts in the crystallized biological materials, such as starch granules [118,119], collagen [27,120,121], and skeletal muscles [122,123]. As discussed in Section 3.1, metallic nanostructures, owing to their SPR, are known to enhance the SHG of the surrounding materials [27,28,31,69].…”

Section: Inherent Harmonic Generationsmentioning

confidence: 99%

Nonlinear plasmonic imaging techniques and their biological applications

et al. 2016

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Nonlinear optics, when combined with microscopy, is known to provide advantages including novel contrast, deep tissue observation, and minimal invasiveness. In addition, special nonlinearities, such as switch on/off and saturation, can enhance the spatial resolution below the diffraction limit, revolutionizing the field of optical microscopy. These nonlinear imaging techniques are extremely useful for biological studies on various scales from molecules to cells to tissues. Nevertheless, in most cases, nonlinear optical interaction requires strong illumination, typically at least gigawatts per square centimeter intensity. Such strong illumination can cause significant phototoxicity or even photodamage to fragile biological samples. Therefore, it is highly desirable to find mechanisms that allow the reduction of illumination intensity. Surface plasmon, which is the collective oscillation of electrons in metal under light excitation, is capable of significantly enhancing the local field around the metal nanostructures and thus boosting up the efficiency of nonlinear optical interactions of the surrounding materials or of the metal itself. In this mini-review, we discuss the recent progress of plasmonics in nonlinear optical microscopy with a special focus on biological applications. The advancement of nonlinear imaging modalities (including incoherent/ coherent Raman scattering, two/three-photon luminescence, and second/third harmonic generations that have been amalgamated with plasmonics), as well as the novel subdiffraction limit imaging techniques based on nonlinear behaviors of plasmonic scattering, is addressed.

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Section: Inherent Harmonic Generationsmentioning

confidence: 99%

Nonlinear plasmonic imaging techniques and their biological applications

et al. 2016

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“…Optical microscopy with SHG has been widely used for imaging collagen, 150-153 myocytes 152,154 and plants. [155][156][157] SHG is a resonant scattering process as shown in Fig. 3(d).…”

Section: Shg Microscopymentioning

confidence: 99%

“…158 Molecular structures having a symmetric distribution of chirality in the membrane do not give rise to SHG signal: only an ordered asymmetric distribution of chiral molecules in the membranes is responsible for SHG. 115 SHG can also be produced in biogenic crystal structures, for example, in calcite or starch granules, 155 and bio-photonic crystalline and semi-crystalline structures in living cells. 159 …”

Section: Shg Microscopymentioning

confidence: 99%

Advanced optical microscopy methods for in vivo imaging of sub-cellular structures in thick biological tissues

Chen

Rehman

Sheppard

2014

J. Innov. Opt. Health Sci.

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Optical microscopy has become an indispensable tool for visualizing sub-cellular structures and biological processes. However, scattering in biological tissues is a major obstacle that prevents high-resolution images from being obtained from deep regions of tissue. We review common techniques, such as multiphoton microscopy (MPM) and optical coherence microscopy (OCM), for di®raction limited imaging beyond an imaging depth of 0.5 mm. Novel implementations have been emerging in recent years giving higher imaging speed, deeper penetration, and better image quality. Focal modulation microscopy (FMM) is a novel method that combines confocal spatial ltering with focal modulation to reject out-of-focus background. FMM has demonstrated an imaging depth comparable to those of MPM and OCM, near-real-time image acquisition, and the capability for multiple contrast mechanisms.

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“…Sometimes SHG in biological specimens appears from microcrystalline structures, for example, calcite in the pineal gland of the human brain (Baconnier and Lang 2004), muscle tissue (Chu et al 2004;Greenhalgh et al 2007), collagen (Freund and Deutsch 1986;Stoller et al 2002), and starch granules (Mizutani et al 2000). The SHG from those structures is generated in a similar way as in nonlinear crystals.…”

Section: Second Harmonic Generationmentioning

confidence: 99%

“…A starch granule is a semi-crystalline structure that extends radially outwards from a hilum, the nucleus of the grain. Figure 4 shows the intense SHG image of a potato starch granule, which represents typical starch granules in plants (Mizutani et al 2000;Chu et al 2001;Cox et al 2005), imaged with linear ( Fig. 4a) and circular polarized laser light (Fig.…”

Section: Starch Granule Imagingmentioning

confidence: 99%

Optical microscopy in photosynthesis

et al. 2009

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We present a new laser system and nonlinear microscope, designed for differential nonlinear microscopy. The microscope features time-correlated single photon counting of multiphoton fluorescence generated by an alternating pulse-train of orthogonally polarized pulses. The generated nonlinear signal is separated using home-built electronics. Results are presented on fluorescence-detected nonlinear absorption linear anisotropy (FDNALA) of chloroplasts in Asparagus Sprengerii Regel and of Congo Red-stained cellulose.

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Detection of starch granules in a living plant by optical second harmonic microscopy

Cited by 65 publications

References 8 publications

Nonlinear plasmonic imaging techniques and their biological applications

Nonlinear plasmonic imaging techniques and their biological applications

Advanced optical microscopy methods for in vivo imaging of sub-cellular structures in thick biological tissues

Optical microscopy in photosynthesis

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