Detection of Staphylococcal Cassette Chromosome
 mec
 Type XI Carrying Highly Divergent
 mecA
 ,
 mecI
 ,
 mecR1
 ,
 blaZ
 , and
 ccr
 Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-Resistant
 Staphylococcus aureus

Shore, Anna C.; Deasy, E.C.; Slickers, Peter; Brennan, Gráinne I.; O’Connell, Brian; Monecke, Stefan; Ehricht, Ralf; Coleman, David C.

doi:10.1128/aac.00187-11

Cited by 348 publications

(332 citation statements)

References 76 publications

(80 reference statements)

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“…Study could have included MSSA strains in addition to the MRSA isolates as with regard to infection prevention and therapy, isolates harboring SCCmec element should be treated as MRSA, even though they are identified by PCR to be mec-A negative [22].…”

Section: Limitationsmentioning

confidence: 99%

Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha

Panda

Mahapatra

Mallick

et al. 2016

JCDR

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Introduction: Methicillin resistant Staphylococcus aureus has emerged as an important pathogen in nosocomial and community acquired infections. Accurate and rapid identification of MRSA in clinical specimens is essential for timely decision of effective antimicrobial chemotherapy. Aim:The present study was conducted to compare efficacy of four conventional phenotypic methods, with mec-A based polymerase chain reaction (PCR) for MRSA identification. materials and methods: Methicillin resistance was determined in 200 S.aureus isolates by oxacillin disc diffusion, cefoxitin disc diffusion, Oxacillin Resistance Screening Agar and E-test. The results were compared with mec-A based PCR.Results: Among 200 S.aureus isolates 62 (31%) were positive for mec-A gene by PCR. Cefoxitin disc diffusion, Oxacillin Resistance Screening Agar and E-test showed 100% specificity. Oxacillin disc diffusion had lowest sensitivity (82.5%) and specificity (98.5%) among all. The conventional methods take more time than PCR for diagnosing MRSA. Linezolid, Vancomycin & Dalfopristin were the highly sensitive drugs against MRSA isolates. Conclusion:Cefoxitin disc diffusion, is rapid, simple and cheaper, hence can be used routinely as an alternative to PCR for detection of MRSA in resource constraint laboratories.

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Section: Limitationsmentioning

confidence: 99%

Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha

Panda

Mahapatra

Mallick

et al. 2016

JCDR

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“…As a result, the transpeptidase activity of PBP2a is functional at normally inhibitory concentrations of beta-lactam antibiotics, allowing cell wall synthesis to occur (2)(3)(4). Recently, a novel variant of mecA was identified in Staphylococcus aureus from cattle (5), humans, and a range of other animal species (6) in Denmark, France, The Netherlands, Ireland, Germany, Belgium, and the United Kingdom (5,(7)(8)(9)(10)(11). This subtype was originally designated mecA LGA251 but has since been renamed mecC and shares 70% nucleotide identity with the conventional mecA gene.…”

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confidence: 99%

A Staphylococcus xylosus Isolate with a New mecC Allotype

Harrison

Paterson

Holden

et al. 2013

Antimicrob Agents Chemother

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jRecently, a novel variant of mecA known as mecC (mecA LGA251 ) was identified in Staphylococcus aureus isolates from both humans and animals. In this study, we identified a Staphylococcus xylosus isolate that harbors a new allotype of the mecC gene, mecC1. Whole-genome sequencing revealed that mecC1 forms part of a class E mec complex (mecI-mecR1-mecC1-blaZ) located at the orfX locus as part of a likely staphylococcal cassette chromosome mec element (SCCmec) remnant, which also contains a number of other genes present on the type XI SCCmec. Methicillin resistance in staphylococci is encoded by mecA, encoding the penicillin-binding protein 2a (PBP2a), which has a low affinity for beta-lactam antibiotics (1). As a result, the transpeptidase activity of PBP2a is functional at normally inhibitory concentrations of beta-lactam antibiotics, allowing cell wall synthesis to occur (2-4). Recently, a novel variant of mecA was identified in Staphylococcus aureus from cattle (5), humans, and a range of other animal species (6) in Denmark, France, The Netherlands, Ireland, Germany, Belgium, and the United Kingdom (5, 7-11). This subtype was originally designated mecA LGA251 but has since been renamed mecC and shares 70% nucleotide identity with the conventional mecA gene. The mecC gene is present with its cognate regulators mecI-mecR1, as part of a class E mec complex that shares structural similarity (mecI-mecR1-mecC-blaZ) with a mec gene complex found in Macrococcus caseolyticus (12). The class E complex is present as part of a larger, 29.4-kb, type XI staphylococcal cassette chromosome mec element (SCCmec) inserted at orfX; this element also includes the recombinase genes ccrAB and arsenic resistance genes. In this work, we describe a highly related mecC homolog present in the orfX locus in a Staphylococcus xylosus.A search of the EMBL nucleotide database identified submission of sequences from S. xylosus strain S04009 (13) with a high degree of similarity (Ͼ90%) to mecC (5, 8). However, antimicrobial susceptibility testing of strain S04009 by disk diffusion with oxacillin and cefoxitin showed it to be susceptible to both antibiotics but resistant to penicillin using British Society for Antimicrobial Chemotherapy (BSAC) criteria (version 10.2) (data not shown). Therefore, we submitted the mecC-positive strain S04009 and a mecC-negative S. xylosus isolate, S040010, for whole-genome sequencing to further characterize the mecC-containing region. Illumina library preparation was carried out as described by Quail et al. (14), and HiSeq sequencing was carried out following the manufacturer's standard protocols (Illumina, Inc.). Genome sequencing confirmed the presence of a mecC homolog in S04009 located downstream of the S. xylosus orfX homolog, a region associated with horizontally transferred elements (Fig. 1). Immediately downstream of orfX in S04009 is a 3.3-kb region that shows a high degree of similarity (Ͼ95% nucleotide identity) to the 3= end of the arginine catabolic mobile element (ACME) in the S. aureus USA300 strai...

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“…In humans, mecC MRSA was isolated in hospital patients from Slovenia [9] , Germany [10] , Republic of Ireland [11] , Denmark [12] , Spain [13] [14] and France [15] . Garcia-Garrote et al [14] reported the first mecC MRSA fatal human case in Spain, a patient with sepsis who died in the emergency department.…”

Section: Discussionmentioning

confidence: 99%

Rapid screening of methicillin-resistant Staphylococcus aureus carrying the mecC gene in a Portuguese hospital

Diaz

Afreixo

Ramalheira

et al. 2018

APALM

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Background:The discovery of mecC gene in MRSA isolates and its report in neighboring Spain and France, advices for monitoring its presence in Portuguese hospitals since changes in local epidemiology can occur quickly. The implementation of rapid procedures based on the automated system VITEK2 allow for simple screening of mecC MRSA isolates with the profile of susceptibility to oxacillin and resistance to cefoxitin signaling positive results for detailed analysis.Methods: This works aims to perform a retrospective study for the presence of suspect mecC MRSA in a Portuguese hospital using to the two methodologies included in microbiology laboratories routine. Result:Our results do not suggest the presence of mecC MRSA, but highlight the possibility of introducing a simple method in clinical laboratories with high percentage of S. aureus isolates. Conclusion:The implementation of this simple procedure in the laboratory routine is a rapid and economic way to monitoring the presence of mecC MRSA.

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Detection of Staphylococcal Cassette Chromosome mec Type XI Carrying Highly Divergent mecA , mecI , mecR1 , blaZ , and ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-Resistant Staphylococcus aureus

Cited by 348 publications

References 76 publications

Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha

Evaluation of Genotypic and Phenotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus in a Tertiary Care Hospital of Eastern Odisha

A Staphylococcus xylosus Isolate with a New mecC Allotype

Rapid screening of methicillin-resistant Staphylococcus aureus carrying the mecC gene in a Portuguese hospital

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