Detection of Sri Lankan cassava mosaic virus by loop-mediated isothermal amplification using dried reagents

Uke, Ayaka; Khin, Sophary; Kobayashi, Kazusuke; Satou, Takuma; Kim, Ok-Kyung; Hoat, Trinh Xuan; Natsuaki, Keiko T.; Ugaki, Masashi

doi:10.1016/j.jviromet.2021.114336

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…The nucleic acid spot hybridization technique was also demonstrated to be more effective in the detection of ICMV using CP gene non-radioactive probe (Makeshkumar et al, 2005). Different sets of primers targeting the conserved AC1 and AV1 regions have been used by various groups in India and Southeast Asia to detect ICMV and SLCMV from field-infected samples (Dutt et al, 2005;Anitha et al, 2019;Uke et al, 2022). Multiplex PCR is useful in detecting mixed infections (Patil et al, 2005), and real-time PCR also has been developed for the detection and quantification of viruses from asymptomatic plants (Makeshkumar et al, 2005;Deepthi et al, 2012).…”

Section: Serological and Molecular Diagnosticsmentioning

confidence: 99%

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Cassava mosaic disease in South and Southeast Asia: current status and prospects

Hareesh

Resmi

Sheela

et al. 2023

Front. Sustain. Food Syst.

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Cassava is one of the most important food and industrial crops in Asia, Africa, and South America. Cassava mosaic disease (CMD), caused by cassava mosaic geminivirus, is one of the major constraints to cassava cultivation. In Asia, the disease is caused by the Indian cassava mosaic virus and Sri Lankan cassava mosaic virus. Phytosanitation, vector control, breeding, and genetic engineering strategies have been widely adopted to manage the disease. This study provides a comprehensive review of the disease spread, the development of diagnostic methods, and various approaches employed for the management of CMD in South and Southeast Asia.

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Section: Serological and Molecular Diagnosticsmentioning

confidence: 99%

“…The sensitivity of the LAMP assay is 10,000 times higher than the conventional PCR. A commercial LAMP kit containing dried reagents is stable at room temperature and can be used for the on-site detection of CMGs (Uke et al, 2022).…”

Section: Serological and Molecular Diagnosticsmentioning

confidence: 99%

Cassava mosaic disease in South and Southeast Asia: current status and prospects

Hareesh

Resmi

Sheela

et al. 2023

Front. Sustain. Food Syst.

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“…In some cases, mosaic viruses do not have a significant effect on plant growth and health beyond leaf spots, and some growers tend to grow and maintain infected plants for their ornamental value. An example of this is Abutilon mosaic virus, which affects plants of the genus Abutilon [26]. The variegation of the leaves resulting from infection is one of the main attractions of this plant (see figure 3).…”

Section: Symptoms and Diagnosismentioning

confidence: 99%

Unraveling the complexities of mosaic viruses in modern agriculture: Comprehensive insights into characterization, impacts, diagnostic, treatment and management

Herrera Gálvez,

Bravo-Osorio

2023

Magna Sci. UCEVA

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Mosaic viruses are a constant concern for the agricultural sector. They pose a real threat to both food and ornamental crops, causing huge economic losses and even threatening food security in many regions. In this article, we will present a general overview of these viruses: their characteristics, transmission mechanisms, effects on crops and available control methods. We will see that one of the main difficulties in dealing with mosaic viruses is their diversity and wide host range. In addition, the lack of effective treatment alternatives and the practical challenges of diagnosing different mosaic virus species require constant epidemiological vigilance to prevent their spread. We will first present a general characterisation of mosaic viruses as an informal group of viruses belonging to tens of different taxa. We will then review the main symptoms of mosaic virus infection (hence the name "mosaic"), diagnostic methods, host range, transmission mechanisms and treatment options. Secondly, we will discuss the impact of these viruses on ornamental and food crops. Finally, we will look at some possible strategies for infection management and control.

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“…The sensitivity of the developed assay compared to the standard PCR, was up to 10,000 times greater and could enable in early diagnostics of this plant disease. Also, using chemicals from a commercial kit that were intended to be stable when dried, a technique was created that enabled for on-the-go analysis without the use of a thermal cycler ( Uke et al., 2022 ). A reverse transcription LAMP test for identification of Wisteria vein mosaic virus which affects wisteria-a woody, deciduous, ornamental flowering plant was studied.…”

Section: Modern Biosensor Technology For Plant Pathogen Detectionmentioning

confidence: 99%

A review of recent advances in plant-pathogen detection systems

Patel¹,

Mitra²,

Vinchurkar³

et al. 2022

Heliyon

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Detection of Sri Lankan cassava mosaic virus by loop-mediated isothermal amplification using dried reagents

Cited by 7 publications

References 15 publications

Cassava mosaic disease in South and Southeast Asia: current status and prospects

Cassava mosaic disease in South and Southeast Asia: current status and prospects

Unraveling the complexities of mosaic viruses in modern agriculture: Comprehensive insights into characterization, impacts, diagnostic, treatment and management

A review of recent advances in plant-pathogen detection systems

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