Detection of specific IgE against linear epitopes from Gal d 1 has additional value in diagnosing hen’s egg allergy in adults

Ehlers, Anna; Otten, Henny G.; Wierzba, Eva; Flugge, U; Le, Thuy‐My; Knulst, André C.; Suer, Waltraud

doi:10.1111/cea.13730

Cited by 12 publications

(6 citation statements)

References 25 publications

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“…Some of the peptides identified in these studies have been reported to be useful to assess the epitope-recognition profiles of antibody repertoires for different isotypes [ 60 , 61 ], for diagnosis [ 52 •, 55 , 62 •, 63 ] or to stratify patients regarding sensitivity to challenge and outcome of oral immunotherapy [ 51 , 64 •, 65 ]. Machine learning algorithms combining epitope and antigen-specific IgE levels in the first 2 to 3 years of life improved the accuracy of predicting peanut allergy at age 4 + years [ 66 ].…”

Section: Advantage Of Human Ige Monoclonal Antibodies For Epitope Map...mentioning

confidence: 99%

“…While linear epitopes are common in food allergens due to their processing in the digestive tract and/or before ingestion (e.g., thermal treatment), conformational or discontinuous epitopes also exist in foods [ 50 , 63 ], and are the most common epitopes in inhalant allergens. Conformational epitopes involve amino acids that are non-contiguous in the amino acid sequence but are close in the space due to the protein folding.…”

Section: Advantage Of Human Ige Monoclonal Antibodies For Epitope Map...mentioning

confidence: 99%

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Human Monoclonal IgE Antibodies—a Major Milestone in Allergy

Smith

Chruszcz

Chapman³

et al. 2022

Curr Allergy Asthma Rep

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Purpose of Review Bound to its high affinity receptor on mast cells and basophils, the IgE antibody molecule plays an integral role in the allergic reaction. Through interactions with the allergen, it provides the sensitivity and specificity parameters for cell activation and mediator release that produce allergic symptoms. Advancements in human hybridoma technologies allow for the generation and molecular definition of naturally occurring allergen-specific human IgE monoclonal antibodies. Recent Findings A high-resolution structure of dust mite allergen Der p 2 in complex with Fab of the human IgE mAb 2F10 was recently determined using X-ray crystallography. The structure reveals the fine molecular details of IgE 2F10 binding its 750 Å2 conformational epitope on Der p 2. Summary This review provides an overview of this major milestone in allergy, the first atomic resolution structure of an authentic human IgE epitope. The molecular insights that IgE epitopes provide will allow for structure-based design approaches to the development of novel diagnostics, antibody therapeutics, and immunotherapies.

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Section: Advantage Of Human Ige Monoclonal Antibodies For Epitope Map...mentioning

confidence: 99%

Section: Advantage Of Human Ige Monoclonal Antibodies For Epitope Map...mentioning

confidence: 99%

Human Monoclonal IgE Antibodies—a Major Milestone in Allergy

Smith

Chruszcz

Chapman³

et al. 2022

Curr Allergy Asthma Rep

View full text Add to dashboard Cite

show abstract

“…These results also provide preliminary basic research for the design of epitope vaccine and preparation of monoclonal antibody with highly conserved peptide epitopes ( 31 ). Similarly, Anna et al found that linear epitopes were important for allergen identification through SDS-PAGE and WB under denaturing conditions ( 32 ). Wang et al also found that two highly conserved linear epitopes of Hexon Protein could help design new structure-based epitope vaccines or therapeutic vaccines ( 33 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of peptides sequence and conformation contributed to potential allergenicity of main allergens in yogurts

Huang

Yang

et al. 2023

Front. Nutr.

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Yogurts provide a good source of nutrition and may induce tolerance in people with cow’s milk allergy (CMA). This study aimed to investigate the IgE-binding capacity of main allergens in the different yogurts which provide a reference for people with a high risk of CMA, and analyze the epitopes of major allergen peptides in yogurt. We assessed the degradation and the allergenic properties of major allergens in six commercial yogurts and fresh milk. The degradation of major allergens was analyzed by SDS-PAGE and RP-HPLC. Western blot and ELISA experiments detected allergenic characteristics by using specific sera. The results showed that β-lactoglobulin (Bos d 5) and α-lactalbumin (Bos d 4) were obviously degraded in yogurts but caseins were still present in abundance, which indicated that the proteases in yogurts were specific to whey proteins. IgE and IgG binding ability of major allergens were obviously reduced in yogurts, especially GuMi yogurt. In addition, 17 peptides of major allergens in GuMi yogurt were identified by LC-MS/MS and most of them were located in the interior of the spatial structure of proteins. Among them, 8 peptides had specific biological functions for health benefits, such as antibacterial, antioxidant, and ACE-inhibitory. We also found that 6 and 14 IgE epitopes of Bos d 5 and caseins were destroyed in GuMi yogurt, which could lead to the reduction of IgE-binding capacity. Meanwhile, peptides [Bos d 5 (AA15–40), Bos d 9 (AA120–151, AA125–151)] also preserved T cell epitopes, which might also induce the development of oral tolerance. Therefore, this study suggested that the sequence and conformation of peptides in yogurts contributed to hypoallergenicity.

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“…This coincided with a number of studies evaluating the burden of peanut allergy and calling for improved protection for people with food allergy 69‐71 . Investigation of component IgE as a marker for ingestion, an immunological target and a discriminator with added diagnostic value also continues to be a busy area of science 72‐79 . Characteristics of infant food anaphylaxis were defined in one of the most extensive published series—hives, hypotonia and hypotension were common features in a French series with 61 infant cases, compared with 386 cases in preschool children 80 .…”

Section: Food Allergymentioning

confidence: 98%

Developments in the field of allergy in 2020 through the eyes of Clinical and Experimental Allergy

Boyle

Shamji

2021

Clin Experimental Allergy

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Detection of specific IgE against linear epitopes from Gal d 1 has additional value in diagnosing hen’s egg allergy in adults

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 12 publications

References 25 publications

Human Monoclonal IgE Antibodies—a Major Milestone in Allergy

Human Monoclonal IgE Antibodies—a Major Milestone in Allergy

Identification of peptides sequence and conformation contributed to potential allergenicity of main allergens in yogurts

Developments in the field of allergy in 2020 through the eyes of Clinical and Experimental Allergy

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