Detection of specific <i>Mycoplasma conjunctivae</i> antibodies in the sera of sheep 
with infectious keratoconjunctivitis

Belloy, Luc; Giacometti, Marco; Abdo, El-Mostafa; Nicolet, Jacques; Krawinkler, M.; Janovský, Martin; Bruderer, Urs; Frey, Joachim

doi:10.1051/vetres:2001118

Cited by 18 publications

(9 citation statements)

References 14 publications

(18 reference statements)

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“…The humoral immune response against the M. conjunctivae infection was assessed in sera with an indirect ELISA based on the Tween 20 membrane proteins extract of the M. conjunctivae strain HRC/581T (64). This ELISA showed good sensitivity and specificity for M. conjunctivae antibodies in sheep and has also been validated for alpine chamois (Rupicapra rupicapra) (10,15).…”

Section: Methodsmentioning

confidence: 99%

Postepizootic Persistence of Asymptomatic Mycoplasma conjunctivae Infection in Iberian Ibex

Fernández-Aguilar

Cabezón

Granados

et al. 2017

Appl Environ Microbiol

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The susceptibility of the Iberian ibex (Capra pyrenaica) to Mycoplasma conjunctivae ocular infection and the changes in their interaction over time were studied in terms of clinical outcome, molecular detection, and IgG immune response in a captive population that underwent a severe infectious keratoconjunctivitis (IKC) outbreak. Mycoplasma conjunctivae was detected in the Iberian ibex, coinciding with the IKC outbreak. Its prevalence had a decreasing trend in 2013 that was consistent with the clinical resolution (August, 35.4%; September, 8.7%; November, 4.3%). Infections without clinical outcome were, however, still detected in the last handling in November. Sequencing and cluster analyses of the M. conjunctivae strains found 1 year later in the ibex population confirmed the persistence of the same strain lineage that caused the IKC outbreak but with a high prevalence (75.3%) of mostly asymptomatic infections and with lower DNA load of M. conjunctivae in the eyes (mean quantitative PCR [qPCR] cycle threshold [C T ], 36.1 versus 20.3 in severe IKC). Significant age-related differences of M. conjunctivae prevalence were observed only under IKC epizootic conditions. No substantial effect of systemic IgG on M. conjunctivae DNA in the eye was evidenced with a linear mixed-models selection, which indicated that systemic IgG does not necessarily drive the resolution of M. conjunctivae infection and does not explain the epidemiological changes observed. The results show how both epidemiological scenarios, i.e., severe IKC outbreak and mostly asymptomatic infections, can consecutively occur by entailing mycoplasma persistence.IMPORTANCE Mycoplasma infections are reported in a wide range of epidemiological scenarios that involve severe disease to asymptomatic infections. This study allows a better understanding of the transition between two different Mycoplasma conjunctivae epidemiological scenarios described in wild host populations and highlights the ability of M. conjunctivae to adapt, persist, and establish diverse interactions with its hosts. The proportion of asymptomatic and clinical M. conjunctivae infections in a host population may not be regarded only in response to intrinsic host species traits (i.e., susceptibility) but also to a specific host-pathogen interaction, which in turn influences the infection dynamics. Both epidemic infectious keratoconjunctivitis and a high prevalence of asymptomatic M. conjunctivae infections may occur in the same host population, depending on the circulation of M. conjunctivae, its maintenance, and the progression of the host-pathogen interactions.

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Section: Methodsmentioning

confidence: 99%

Postepizootic Persistence of Asymptomatic Mycoplasma conjunctivae Infection in Iberian Ibex

Fernández-Aguilar

Cabezón

Granados

et al. 2017

Appl Environ Microbiol

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“…An indirect enzyme-linked immunosorbent assay (ELISA) method based on the Tween 20 extracted fraction of M. conjunctivae strain HRC/581 T has recently been developed to detect M. conjunctivae antibodies in sera of domestic sheep (7). For the analysis of the sheep sera, serum is diluted 1/10, applied to the plates and analysed using a commercially-available monoclonal antibody directed against ruminant immunoglobulin G (IgG) conjugate to horseradish peroxidase, according to the instructions provided by the manufacturer.…”

Section: Laboratory Diagnosismentioning

confidence: 99%

“…The value of each sample is expressed as a percentage of a positive reference standard, taking a negative reference serum as zero value. The cut-off level (37%) was set 3 standard deviations higher than the mean value for negative controls according to the test validation (7). At present, studies are being performed to develop and evaluate an ELISA to be used in Alpine chamois (28).…”

Section: Laboratory Diagnosismentioning

confidence: 99%

“…Therefore, studies are required to evaluate the distribution of M. conjunctivae infection in domestic sheep in several countries and to assess the risk of IKC spillover from domestic animals to wildlife. For this purpose, new molecular and serological methods have recently been developed for the rapid and specific detection of M. conjunctivae infections (7,26). In addition, further studies are needed to improve understanding of mechanisms of interspecific transmission of the pathogen and to evaluate infection maintenance in both domestic and wild Caprinae populations.…”

Section: Perspectivesmentioning

confidence: 99%

See 1 more Smart Citation

Infectious keratoconjunctivitis of ibex, chamois and other Caprinae

Giacometti¹,

Janovský²,

Belloy³

et al. 2002

Rev. Sci. Tech. OIE

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“…Recent studies detected several specific antigens of M. conjunctivae, including proteins of 175, 73, 68, 60 and 33 kDa, which have been exploited to develop specific serological tests to detect M. conjunctivae infection (Degiorgis et al, 2000;Belloy et al, 2001). To study the antigenic proteins in more detail, we screened a bacteriophage l-based expression gene library of the M. conjunctivae type strain HRC/581 T using rabbit serum raised against a whole cell preparation of M. conjunctivae.…”

Section: Introductionmentioning

confidence: 99%

Characterization of LppS, an adhesin of Mycoplasma conjunctivae

Belloy

Vilei

Giacometti³

et al. 2003

Microbiology

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A serine-rich membrane protein named LppS from Mycoplasma conjunctivae, the aetiological agent of infectious keratoconjunctivitis (IKC) of domestic and wild Caprinae, was characterized. Gene cloning and sequence analysis of the lppS gene revealed that it encoded a membrane protein precursor. The protein had a typical signal sequence and a signal peptidase II cleavage site followed by a cysteine residue representing a potential acylation site. The mature LppS protein had an apparent molecular mass of 150 kDa and was found in the detergent-associated fraction of Tween 20 extracted M. conjunctivae proteins. It possessed a serine-rich domain of 41 aa with 37 (90?2 %) serine residues. Twenty-seven of these serine residues were contiguous. The protein adhered to lamb joint synovial cells. Using an in vitro adhesion model, Fab fragments from IgG directed against recombinant purified LppS were shown to specifically inhibit adhesion of M. conjunctivae to lamb cells. Thus, LppS is likely to be an adhesin of M. conjunctivae that may play an important role in the pathogenesis of IKC.

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Detection of specific Mycoplasma conjunctivae antibodies in the sera of sheep with infectious keratoconjunctivitis

Cited by 18 publications

References 14 publications

Postepizootic Persistence of Asymptomatic Mycoplasma conjunctivae Infection in Iberian Ibex

Postepizootic Persistence of Asymptomatic Mycoplasma conjunctivae Infection in Iberian Ibex

Infectious keratoconjunctivitis of ibex, chamois and other Caprinae

Characterization of LppS, an adhesin of Mycoplasma conjunctivae

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