Detection of specific antibodies against SARS-CoV-2 spike protein <i>via</i> ultra-sensitive bio-functionalized carbonnitride-reduced graphene oxide electrochemical immunosensing platform in real samples

Sadique, Mohd. Abubakar; Yadav, Shalu; Ranjan, Pushpesh; Chouhan, Raghuraj Singh; Jerman, Ivan; Kumar, Ashok; Saigal, Saurabh; Khadanga, Sagar; Khan, Raju; Srivastava, Avanish K.

doi:10.1039/d3ma00399j

Cited by 2 publications

(1 citation statement)

References 71 publications

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“…Andre Geim and Konstantin Novoselov discovered graphene in 2004. 53 Since then, it has been followed by other materials like hexagonal boron nitride (h-BN), graphite carbon nitride (g-C 3 N 4 ), 54 transition metal dichalcogenides (TMDs) like molybdenum disulfide (MoS 2 ) 55,56 and tungsten disulfide (WS 2 ), 57–59 transition metal oxides (TMOs) like MoO 3 , WO 3 , and MnO 2 , and MXenes. 60–63 Gogosti and co-workers identified MXenes as an extensive family of 2D transition metal carbides, nitrides, or carbonitrides in 2011.…”

Section: Introduction: a Demi-decade In Combatting The Sars-cov-2 Out...mentioning

confidence: 99%

Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

Sadique,

Yadav,

Khan

et al. 2024

Chem. Soc. Rev.

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Section: Introduction: a Demi-decade In Combatting The Sars-cov-2 Out...mentioning

confidence: 99%

Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

Sadique,

Yadav,

Khan

et al. 2024

Chem. Soc. Rev.

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Hydroxyapatite-Gold Modified Screen-Printed Carbon Electrode for Selective SARS-CoV-2 Antibody Immunosensor

Syafira,

Devi,

Gaffar

et al. 2024

ACS Appl. Bio Mater.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), or coronavirus disease 2019 , is still spreading worldwide; therefore, the need for rapid and accurate detection methods remains relevant to maintain the spread of this infectious disease. Electrochemical immunosensors are an alternative method for the rapid detection of the SARS-CoV-2 virus. Herein, we report the development of a screen-printed carbon electrode immunosensor using a hydroxyapatite-gold nanocomposite (SPCE/HA-Au) directly spray-coated with the immobilization receptor binding domain (RBD) Spike to increase the conductivity and surface electrode area. The HA-Au composite synthesis was optimized using the Box−Behnken method, and the resulting composite was characterized by UV−vis spectrophotometry, TEM-EDX, and XRD analysis. The specific interaction of RBD Spike with immunoglobulin G (IgG) antibodies was evaluated by differential pulse voltammetry and electrochemical impedance spectroscopy methods in a [Fe(CN) 6 ] 4−/3− solution redox system. The IgG was detected with a detection limit of 0.0561 pg mL −1 , and the immunosensor had selectivity and stability of 103−122% and was stable until week 7 with the influence of storage conditions. Also, the immunosensor was tested using real samples from human serum, where the results were confirmed using the chemiluminescent microparticle immunoassay (CMIA) method and showed satisfactory results. Therefore, the developed electrochemical immunosensor can rapidly and accurately detect SARS-CoV-2 antibodies.

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Detection of specific antibodies against SARS-CoV-2 spike protein via ultra-sensitive bio-functionalized carbonnitride-reduced graphene oxide electrochemical immunosensing platform in real samples

Abstract: Ultrasensitive immunosensing device based on bio-functionalized C3N4/RGO nanocomposite allows for exact differentiation between positive and negative real COVID-19 patient samples.

Cited by 2 publications

References 71 publications

Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

Hydroxyapatite-Gold Modified Screen-Printed Carbon Electrode for Selective SARS-CoV-2 Antibody Immunosensor

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