Detection of Spatial-Specific Phytochrome Responses Using Targeted Expression of Biliverdin Reductase in Arabidopsis

Warnasooriya, Sankalpi N.; Montgomery, Beronda L.

doi:10.1104/pp.108.127050

Cited by 36 publications

(98 citation statements)

References 70 publications

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“…1C). Furthermore, similar to the results under FRc, 40 mesophyll-localized phyA is primarily responsible for the phytochrome-dependent induction of anthocyanin accumulation under Bc (Fig. 1C).…”

Section: Resultssupporting

confidence: 86%

“…Based on prior findings that CAB3::pBVR lines are impaired significantly in anthocyanin accumulation under FRc and Rc 40 and the previous observations that phyA can impact anthocyanin levels under B (see discussion above), we measured anthocyanin levels under Bc in transgenic CAB3::pBVR lines to determine whether anthocyanin levels were reduced in the absence of mesophyll-localized phys, as they were under FRc and Rc. We determined that a representative CAB3::pBVR line, i.e., CAB3::pBVR2, accumulates less anthocyanin than does the No-0 WT parent under Bc independent of the presence or absence of sucrose in the growth media (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…40 Single apophytochrome mutants obtained from the Salk T-DNA insertion mutant collection were assessed for the absence of respective transcript accumulation by RT-PCR analysis. In the selected T-DNA insertion mutants, the T-DNA is inserted in an exon region of each apophytochrome gene ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Although total levels of pools of phytochromes and cryptochromes contribute to the photoregulation of distinct photoreceptor-dependent responses. [37][38][39][40] Mesophyll-localized phytochromes are associated with the photoperiodic control of flowering, 38 the photoregulation of hypocotyl elongation, 39,40 and light-dependent pigment accumulation. 40 Cryptochrome 2 in vascular bundles regulates flowering.…”

Section: Resultsmentioning

confidence: 99%

“…[37][38][39][40] Mesophyll-localized phytochromes are associated with the photoperiodic control of flowering, 38 the photoregulation of hypocotyl elongation, 39,40 and light-dependent pigment accumulation. 40 Cryptochrome 2 in vascular bundles regulates flowering. 37 Recent results from the analyses of transgenic lines exhibiting mesophyll-localized phytochrome deficiencies indicated that mesophyll-localized phyA is responsible specifically for the photoregulation of a number of FR-dependent phenotypes, including the inhibition of hypocotyl elongation, the FR block-to-greening response and the FR-mediated induction of anthocyanin accumulation.…”

Section: Resultsmentioning

confidence: 99%

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Tissue- and isoform-specific phytochrome regulation of light-dependent anthocyanin accumulation in Arabidopsis thaliana

Warnasooriya

Porter

Montgomery

2011

Plant Signaling & Behavior

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Tissue- and isoform-specific phytochrome regulation of light-dependent anthocyanin accumulation in Arabidopsis thaliana

Warnasooriya

Porter

Montgomery

2011

Plant Signaling & Behavior

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Nuclear‐encoded sigma factor 6 (SIG6) is involved in the block of greening response in Arabidopsis thaliana

Alameldin

Hernandez

et al. 2020

American J of Botany

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Premise Light is critical in the ability of plants to accumulate chlorophyll. When exposed to far‐red (FR) light and then grown in white light in the absence of sucrose, wild‐type seedlings fail to green in a response known as the FR block of greening (BOG). This response is controlled by phytochrome A through repression of protochlorophyllide reductase‐encoding (POR) genes by FR light coupled with irreversible plastid damage. Sigma (SIG) factors are nuclear‐encoded proteins that contribute to plant greening and plastid development through regulating gene transcription in chloroplasts and impacting retrograde signaling from the plastid to nucleus. SIGs are regulated by phytochromes, and the expression of some SIG factors is reduced in phytochrome mutant lines, including phyA. Given the association of phyA with the FR BOG and its regulation of SIG factors, we investigated the potential regulatory role of SIG factors in the FR BOG response. Methods We examined FR BOG responses in sig mutants, phytochrome‐deficient lines, and mutant lines for several phy‐associated factors. We quantified chlorophyll levels and examined expression of key BOG‐associated genes. Results Among six sig mutants, only the sig6 mutant significantly accumulated chlorophyll after FR BOG treatment, similar to the phyA mutant. SIG6 appears to control protochlorophyllide accumulation by contributing to the regulation of tetrapyrrole biosynthesis associated with glutamyl‐tRNA reductase (HEMA1) function, select phytochrome‐interacting factor genes (PIF4 and PIF6), and PENTA1, which regulates PORA mRNA translation after FR exposure. Conclusions Regulation of SIG6 plays a significant role in plant responses to FR exposure during the BOG response.

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Transcriptome and phenotyping analyses support a role for chloroplast sigma factor 2 in red‐light‐dependent regulation of growth, stress, and photosynthesis

Strand

Kramer

et al. 2018

Plant Direct

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Sigma factor (SIG) proteins contribute to promoter specificity of the plastid‐encoded RNA polymerase during chloroplast genome transcription. All six members of the SIG family, that is, SIG1–SIG6, are nuclear‐encoded proteins targeted to chloroplasts. Sigma factor 2 (SIG2) is a phytochrome‐regulated protein important for stoichiometric control of the expression of plastid‐ and nuclear‐encoded genes that impact plastid development and plant growth and development. Among SIG factors, SIG2 is required not only for transcription of chloroplast genes (i.e., anterograde signaling), but also impacts nuclear‐encoded, photosynthesis‐related, and light signaling‐related genes (i.e., retrograde signaling) in response to plastid functional status. Although SIG2 is involved in photomorphogenesis in Arabidopsis, the molecular bases for its role in light signaling that impacts photomorphogenesis and aspects of photosynthesis have only recently begun to be investigated. Previously, we reported that SIG2 is necessary for phytochrome‐mediated photomorphogenesis specifically under red (R) and far‐red light, thereby suggesting a link between phytochromes and nuclear‐encoded SIG2 in light signaling. To explore transcriptional roles of SIG2 in R‐dependent growth and development, we performed RNA sequencing analysis to compare gene expression in sig2‐2 mutant and Col‐0 wild‐type seedlings at two developmental stages (1‐ and 7‐day). We identified a subset of misregulated genes involved in growth, hormonal cross talk, stress responses, and photosynthesis. To investigate the functional relevance of these gene expression analyses, we performed several comparative phenotyping tests. In these analyses, strong sig2 mutants showed insensitivity to bioactive GA3, high intracellular levels of hydrogen peroxide (H2O2) indicative of a stress response, and specific defects in photosynthesis, including elevated levels of cyclic electron flow (CEF) and nonphotochemical quenching (NPQ). We demonstrated that SIG2 regulates a broader range of physiological responses at the molecular level than previously reported, with specific roles in red‐light‐mediated photomorphogenesis.

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Detection of Spatial-Specific Phytochrome Responses Using Targeted Expression of Biliverdin Reductase in Arabidopsis

Cited by 36 publications

References 70 publications

Tissue- and isoform-specific phytochrome regulation of light-dependent anthocyanin accumulation in Arabidopsis thaliana

Tissue- and isoform-specific phytochrome regulation of light-dependent anthocyanin accumulation in Arabidopsis thaliana

Nuclear‐encoded sigma factor 6 (SIG6) is involved in the block of greening response in Arabidopsis thaliana

Transcriptome and phenotyping analyses support a role for chloroplast sigma factor 2 in red‐light‐dependent regulation of growth, stress, and photosynthesis

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