Detection of soluble biomarkers of pancreatic cancer in endoscopic ultrasound-guided fine-needle aspiration samples

Souche, Regis; Tosato, Guillaume; Rivière, Benjamin; Valats, Jean-Christophe; Debourdeau, Antoine; Flori, Nicolas; Pourquier, Didier; Fabre, Jean‐Michel; Assenat, Éric; Colinge, Jacques; Turtoï, Andrei

doi:10.1055/a-1550-2503

Cited by 5 publications

(6 citation statements)

References 17 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sekita-Hatakeyama et al ( 26 ) performed NGS for liquid-based cytological specimens to assess the mutations of KRAS, TP53, CDKN2A, SMAD4, and PIK3CA, which could improve the diagnostic efficiency of FNA for pancreatic tumors. The PDAC protein found by Souche et al ( 27 ) in liquid-based cytological specimens could be a potential biomarker for pancreatic tumors.…”

Section: Discussionmentioning

confidence: 91%

Detection value of endoscopic ultrasound-guided 19G fine-needle wet-heparinized suction for pancreatic solid tumors: a randomized controlled trial

Xu¹,

Lü²,

Xu³

et al. 2023

Gland Surg

View full text Add to dashboard Cite

Background: Conventional endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) has some inevitable flaws in the detection of pancreatic solid tumors, such as an incomplete histological structure of the obtained pancreatic biopsy tissues and blood coagulation. Heparin can prevent blood coagulation, thus improving the structural integrity of the specimen. However, whether the combination of EUS-FNA and wet heparin can improve the detection of pancreatic solid tumors needs to be further explored. Hence, this study aimed to compare the EUS-FNA combined with wet heparin and the conventional EUS-FNA, and analyze the detection value of EUS-FNA combined with wet heparin for pancreatic solid tumors. Methods:The clinical data of 52 patients with pancreatic solid tumors who had received EUS-FNA at the Wuhan Fourth Hospital from August 2019 to April 2021 were selected. Patients were divided into a heparin group and a conventional wet-suction group using a randomized number table. The total length of biopsy tissue strips, total length of white tissue core in pancreatic biopsy lesions [according to macroscopic onsite evaluation (MOSE)], total length of white tissue core in each biopsy tissue, erythrocyte contamination in the paraffin sections, and postoperative complications were compared between the groups. The receiver operating characteristic curve was used to reflect the detection value of EUS-FNA combined with wet heparin for pancreatic solid tumors. Results:The heparin group had a longer total length of biopsy tissue strips (P<0.05) and total length of white tissue core (P<0.05) than the conventional group. There was a positive correlation between the total length of white tissue core and the total length of biopsy tissue strips in both groups (conventional wetsuction group: r=0.470, P<0.05; heparin group: r=0.433, P<0.05). The heparin group had milder erythrocyte contamination in the paraffin sections (P<0.05). The total length of white tissue core in the heparin group had the highest diagnostic performance, with a Youden index of 0.819 [area under the curve (AUC) =0.944].Conclusions: Our research shows that wet-heparinized suction improves the quality of pancreatic solid tumor tissue biopsy obtained by 19G fine-needle aspiration and is a safe and efficient aspiration method in conjunction with MOSE for tissue biopsy.

show abstract

Section: Discussionmentioning

confidence: 91%

Detection value of endoscopic ultrasound-guided 19G fine-needle wet-heparinized suction for pancreatic solid tumors: a randomized controlled trial

Xu¹,

Lü²,

Xu³

et al. 2023

Gland Surg

View full text Add to dashboard Cite

show abstract

“…Detection of proteins in the LN FNA supernatant that were not detectable from capillary blood or serum supports the conclusion that we are sampling lymph node tissue and/or lymph-associated proteins, as opposed to blood contaminants introduced by the procedure. There has been interest, particularly in cancer, in proteomic analysis of FNA supernatants -from possible malignant tissue or metastases containing LNs -to screen for the presence of cancer cells [44][45][46]. Such studies focus on detecting cellular abundance (i.e.…”

Section: Discussionmentioning

confidence: 99%

Fine needle aspiration of human lymph nodes reveals cell populations and soluble interactors pivotal to immunological priming

Provine,

Al‐Diwani,

Agarwal

et al. 2024

Eur J Immunol

View full text Add to dashboard Cite

Lymph node (LN) fine needle aspiration (LN FNA) represents a powerful technique for minimally invasive sampling of human LNs in vivo and has been used effectively to directly study aspects of the human germinal center response. However, systematic deep phenotyping of the cellular populations and cell‐free proteins recovered by LN FNA has not been performed. Thus, we studied human cervical LN FNAs as a proof‐of‐concept and used single‐cell RNA‐sequencing and proteomic analysis to benchmark this compartment, define the purity of LN FNA material, and facilitate future studies in this immunologically pivotal environment. Our data provide evidence that LN FNAs contain bone‐fide LN‐resident innate immune populations, with minimal contamination of blood material. Examination of these populations reveals unique biology not predictable from equivalent blood‐derived populations. LN FNA supernatants represent a specific source of lymph‐ and lymph node‐derived proteins, and can, aided by transcriptomics, identify likely receptor–ligand interactions. This represents the first description of the types and abundance of immune cell populations and cell‐free proteins that can be efficiently studied by LN FNA. These findings are of broad utility for understanding LN physiology in health and disease, including infectious or autoimmune perturbations, and in the case of cervical nodes, neuroscience.

show abstract

“…A recent study has shown how protein extracts from EUS‐FNAC‐derived fluid can be analyzed by mass spectrometry and proteomics with an application of protein signature score for pancreatic ductal adenocarcinoma screening and diagnosis 74 . Moreover, this signature score could help identify pancreatic ductal adenocarcinoma in a vast majority of EUS‐FNAC inconclusive cases on cytology.…”

Section: Ancillary Techniques With Eus‐fna Icing On the Cakementioning

confidence: 99%

EUS‐guided FNAC in intra‐abdominal lesions: Technique of tissue acquisition, ancillary testing, pearls and perils, and prospects

Kundu

Rana

Suneel

et al. 2023

Diagnostic Cytopathology

View full text Add to dashboard Cite

Endoscopic ultrasound enables visualization of lesions within and in the vicinity of the gastrointestinal tract. Endoscopic ultrasound guided fine needle aspiration cytology (EUS‐FNAC) helps in targeting various luminal and extraluminal lesions both diagnostically and therapeutically. Various intra‐abdominal organs amenable to EUS‐FNA include the gastrointestinal tract (GIT), pancreas, kidney, adrenal gland, liver, bile duct, gallbladder, spleen, and lymph nodes. EUS‐FNAC is mostly done for pancreatic and intra‐abdominal lymph nodal lesions. In the present review, we have discussed various aspects of EUS‐FNAC.

show abstract

Detection of soluble biomarkers of pancreatic cancer in endoscopic ultrasound-guided fine-needle aspiration samples

Cited by 5 publications

References 17 publications

Detection value of endoscopic ultrasound-guided 19G fine-needle wet-heparinized suction for pancreatic solid tumors: a randomized controlled trial

Detection value of endoscopic ultrasound-guided 19G fine-needle wet-heparinized suction for pancreatic solid tumors: a randomized controlled trial

Fine needle aspiration of human lymph nodes reveals cell populations and soluble interactors pivotal to immunological priming

EUS‐guided FNAC in intra‐abdominal lesions: Technique of tissue acquisition, ancillary testing, pearls and perils, and prospects

Contact Info

Product

Resources

About