Detection of soil fungal communities in an alpine primary successional habitat: Does pooling of DNA extracts affect investigations?

Oberkofler, Irmgard; Peintner, Ursula

doi:10.1007/bf03175562

Cited by 9 publications

(9 citation statements)

References 39 publications

(35 reference statements)

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“…The bags were filled with 10 g of sterile soil (irradiated with 1,000 Gy) from the study site. PCR amplification of fungal ITS-rDNA was carried out using the primer pair ITS1F [17] and ITS4 [79] or ITS1F and NL4 [47]; cloning was performed with the Promega pGEM®-T Cloning Kit [48]. Ten clones were identified from each mesh bag.…”

Section: Ingrowth Mesh Bag Experimentsmentioning

confidence: 99%

Fungal Growth and Biomass Development is Boosted by Plants in Snow-Covered Soil

2012

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Soil microbial communities follow distinct seasonal cycles which result in drastic changes in processes involving soil nutrient availability. The biomass of fungi has been reported to be highest during winter, but is fungal growth really occurring in frozen soil? And what is the effect of plant cover on biomass formation and on the composition of fungal communities? To answer these questions, we monitored microbial biomass N, ergosterol, and the amount of fungal hyphae during summer and winter in vegetated and unvegetated soils of an alpine primary successional habitat. The winter fungal communities were identified by rDNA ITS clone libraries. Winter soil temperatures ranged between -0.6°C and -0.1°C in snow-covered soil. We found distinct seasonal patterns for all biomass parameters, with highest biomass concentrations during winter in snow-covered soil. The presence of plant cover had a significant positive effect on the amount of biomass in the soil, but the type of plant cover (plant species) was not a significant factor. A mean hyphal ingrowth of 5.6 m g(-1) soil was detected in snow-covered soil during winter, thus clearly proving fungal growth during winter in snow-covered soil. Winter fungal communities had a typical species composition: saprobial fungi were dominating, among them many basidiomycete yeasts. Plant cover had no influence on the composition of winter fungal communities.

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Section: Ingrowth Mesh Bag Experimentsmentioning

confidence: 99%

Fungal Growth and Biomass Development is Boosted by Plants in Snow-Covered Soil

2012

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“…The ITS sequence of an unknown fungus (GenBank accession no. EF635841) isolated from a soil sample near a glacier in the Austrian Alps (Oberkofler & Peintner, 2008) showed 99 % identity with the strain we isolated from G. montanum. Since no potential host plant was recorded at this locality, this sequence might represent the asexual, saprobic yeast state of T. gei-montani.…”

Section: Genetic Similarities and Possible Errors In Identification Omentioning

confidence: 64%

“…Furthermore, the ITS sequences from T. gei-montani displayed 99 % identity with an unknown Taphrina strain (GenBank accession no. EF635841), isolated from the soil adjacent to Salix herbacea, Austrian Central Alps (Oberkofler & Peintner, 2008).…”

Section: Symptoms Of Infectionmentioning

confidence: 99%

Disentangling identity of species of the genus Taphrina parasitizing herbaceous Rosaceae, with proposal of Taphrina gei-montani sp. nov.

Petrýdesová

Kučera

Bacigálová

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Five strains (CCY 058-007-001 T , CCY 058-007-002, CCY 058-007-003, CCY 058-007-004 and CCY 058-007-005) of a novel parasitic yeast belonging to the genus Taphrina were isolated from leaf tissues of Geum montanum L. (Rosaceae), collected from the Vysok e Tatry Mountains, Slovakia. Genetic analyses revealed that these isolates differ by 15 unique substitutions in the ITS region and by six substitutions in the rns gene from all other species of the genus Taphrina analysed hitherto. The novel strains are also distinguished from all other species of the genus Taphrina by their morphology, biochemical properties and ecology. These strains represent a novel species, for which the name Taphrina gei-montani sp. nov. is proposed. The type strain is CCY 058-007-001 T (=CBS 14159=BU001). The MycoBank number is MB815677. The present study also demonstrates that two distinct species of the genus Taphrina parasitize the herbaceous Rosaceae: Taphrina gei-montani sp. nov. on Geum montanum and Taphrina tormentillae on Potentilla species.

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“…Moreover, this taxon was one of the most abundant mycobionts of Salix herbacea, Bistorta vivipara and of Kobresia myosuroides at this site (Mühlmann et al 2008;Mühlmann and Peintner 2008a, b), and was also detected in the corresponding soil fungal community (Oberkofler and Peintner 2008). Based on Emerenica searches, sequences belonging to T. alpina were never detected in other habitats, thus suggesting adaptation to the alpine environment.…”

Section: Resultsmentioning

confidence: 92%

“…Comments T. atramentaria is common and has a worldwide distribution. On the Rotmoos glacier forefront, it has been detected as mycorrhizal partner of Salix, Kobresia and B. vivipara, and as member of the soil fungal community (Mühlmann et al 2008;Mühlmann and Peintner 2008a, b;Oberkofler and Peintner 2008); T. atramentaria was also reported as Kobresia mycobiont from the Himalaja (Gao and Yang 2010).…”

Section: Figmentioning

confidence: 93%

Tomentella alpina and other tomentelloid taxa fruiting in a glacier valley

Peintner

Dämmrich²

2011

Mycol Progress

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Tomentella is a genus of resupinate basidiomycetes usually fruiting on rotten wood. Ecological studies based on molecular methods have reported many Tomentella species as mycobionts of alpine ectomycorrhizal plants, thus highlighting their importance for plant establishment and development under extreme conditions. For the first time, we report fruiting of eight tomentelloid species in an alpine site, and describe Tomentella alpina as a new species. In the rDNA ITS phylogeny, Tomentella alpina forms a distinct clade in the T. stuposa complex, from which it can be clearly separated based on spore size and shape. Closely related taxa are briefly described, and synonymy of Tomentella fungicola with T. stuposa is rejected. Tomentella alpina was found to be one of the most important mycorrhizal partners of Kobresia myosuroides, Bistorta vivipara and Salix herbacea at this alpine site. The mutualistic association with plants is a very successful life strategy for Tomentella spp. growing in primary successional habitats, where the lack of organic matter is generally a growth-limiting factor.

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Detection of soil fungal communities in an alpine primary successional habitat: Does pooling of DNA extracts affect investigations?

Cited by 9 publications

References 39 publications

Fungal Growth and Biomass Development is Boosted by Plants in Snow-Covered Soil

Fungal Growth and Biomass Development is Boosted by Plants in Snow-Covered Soil

Disentangling identity of species of the genus Taphrina parasitizing herbaceous Rosaceae, with proposal of Taphrina gei-montani sp. nov.

Tomentella alpina and other tomentelloid taxa fruiting in a glacier valley

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