Detection of Single Nucleotide Polymorphisms by a Gold Nanowire‐on‐Film SERS Sensor Coupled with S1 Nuclease Treatment

Yoo, Seung Min; Kang, Taejoon; Kim, Bongsoo; Lee, Sang Yup

doi:10.1002/chem.201003372

Cited by 26 publications

(12 citation statements)

References 40 publications

(68 reference statements)

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“…Among various kinds of nanostructures, NWs have been employed as superb signal transducers for detection of biological and chemical species because the diameters of NWs are comparable to the size of such species . In particular, single‐crystalline noble metal NW‐based surface‐enhanced Raman scattering (SERS) sensors allowed highly sensitive and reproducible detection of diverse biochemical molecules because of their well‐defined architecture . For the ultra‐specific and ultra‐sensitive detection of miRNAs, we applied miRNA‐specific bi‐temperature hybridizations to single‐crystalline Au NW surfaces, where short miRNAs can readily crawl into the narrow hot spots of the PNI sensor for effective SERS detection ( Figure a).…”

Section: Resultsmentioning

confidence: 99%

Ultra‐Specific Zeptomole MicroRNA Detection by Plasmonic Nanowire Interstice Sensor with Bi‐Temperature Hybridization

Kang

Kim

Lee

et al. 2014

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MicroRNAs (miRNAs) are emerging new biomarkers for many human diseases. To fully employ miRNAs as biomarkers for clinical diagnosis, it is most desirable to accurately determine the expression patterns of miRNAs. The optimum miRNA profiling method would feature 1) highest sensitivity with a wide dynamic range for accurate expression patterns, 2) supreme specificity to discriminate single nucleotide polymorphisms (SNPs), and 3) simple sensing processes to minimize measurement variation. Here, an ultra-specific detection method of miRNAs with zeptomole sensitivity is reported by applying bi-temperature hybridizations on single-crystalline plasmonic nanowire interstice (PNI) sensors. This method shows near-perfect accuracy of SNPs and a very low detection limit of 100 am (50 zeptomole) without any amplification or labeling steps. Furthermore, multiplex sensing capability and wide dynamic ranges (100 am-100 pm) of this method allows reliable observation of the expression patterns of miRNAs extracted from human tissues. The PNI sensor offers combination of ultra-specificity and zeptomole sensitivity while requiring two steps of hybridization between short oligonucleotides, which could present the best set of features for optimum miRNA sensing method.

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Section: Resultsmentioning

confidence: 99%

Ultra‐Specific Zeptomole MicroRNA Detection by Plasmonic Nanowire Interstice Sensor with Bi‐Temperature Hybridization

Kang

Kim

Lee

et al. 2014

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“…Lately, various SNPs detection procedures have been introduced. Few of these are surface-enhanced Raman scattering, high-resolution DNA melting, hybridization chain reactions, and single molecular fluorescence [ 90 , 91 , 92 , 93 ]. But all the techniques lack precision and sensitivity to the specific enzyme recognition site.…”

Section: Detection Of Snpsmentioning

confidence: 99%

Application of Nanotechnology for Sensitive Detection of Low-Abundance Single-Nucleotide Variations in Genomic DNA: A Review

et al. 2021

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Single-nucleotide polymorphisms (SNPs) are the simplest and most common type of DNA variations in the human genome. This class of attractive genetic markers, along with point mutations, have been associated with the risk of developing a wide range of diseases, including cancer, cardiovascular diseases, autoimmune diseases, and neurodegenerative diseases. Several existing methods to detect SNPs and mutations in body fluids have faced limitations. Therefore, there is a need to focus on developing noninvasive future polymerase chain reaction (PCR)–free tools to detect low-abundant SNPs in such specimens. The detection of small concentrations of SNPs in the presence of a large background of wild-type genes is the biggest hurdle. Hence, the screening and detection of SNPs need efficient and straightforward strategies. Suitable amplification methods are being explored to avoid high-throughput settings and laborious efforts. Therefore, currently, DNA sensing methods are being explored for the ultrasensitive detection of SNPs based on the concept of nanotechnology. Owing to their small size and improved surface area, nanomaterials hold the extensive capacity to be used as biosensors in the genotyping and highly sensitive recognition of single-base mismatch in the presence of incomparable wild-type DNA fragments. Different nanomaterials have been combined with imaging and sensing techniques and amplification methods to facilitate the less time-consuming and easy detection of SNPs in different diseases. This review aims to highlight some of the most recent findings on the aspects of nanotechnology-based SNP sensing methods used for the specific and ultrasensitive detection of low-concentration SNPs and rare mutations.

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“…When perfectly matched target DNA is hybridized with a DNA probe, it protects the DNA probe from S1 nuclease attack. Then, the Raman carrier Cy5 retains its function to provide a strong SERS signal as shown in Figure 11 [115]. …”

Section: Recent Development In the Use Of Gold Nanoparticles Basedmentioning

confidence: 99%

New Gold Nanostructures for Sensor Applications: A Review

et al. 2014

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Gold based structures such as nanoparticles (NPs) and nanowires (NWs) have widely been used as building blocks for sensing devices in chemistry and biochemistry fields because of their unusual optical, electrical and mechanical properties. This article gives a detailed review of the new properties and fabrication methods for gold nanostructures, especially gold nanowires (GNWs), and recent developments for their use in optical and electrochemical sensing tools, such as surface enhanced Raman spectroscopy (SERS).

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Detection of Single Nucleotide Polymorphisms by a Gold Nanowire‐on‐Film SERS Sensor Coupled with S1 Nuclease Treatment

Cited by 26 publications

References 40 publications

Ultra‐Specific Zeptomole MicroRNA Detection by Plasmonic Nanowire Interstice Sensor with Bi‐Temperature Hybridization

Ultra‐Specific Zeptomole MicroRNA Detection by Plasmonic Nanowire Interstice Sensor with Bi‐Temperature Hybridization

Application of Nanotechnology for Sensitive Detection of Low-Abundance Single-Nucleotide Variations in Genomic DNA: A Review

New Gold Nanostructures for Sensor Applications: A Review

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