Abstract:Abstract:To evaluate scallop safety in the Guangzhou seafood market, contents of shellfish toxins in adductor muscle, mantle skirts, gills and visceral mass of scallops were examined using enzyme-linked immunosorbent assay (ELISA) and mouse unit assay. The results showed that: paralytic shellfish poisoning contents were up to 37.44 mg/100 g by ELISA and 319.99 MU/100 g by mouse unit assay, which did not exceed the limits of national standards (80 mg/100g and 400 MU/100 g); the contents of diarrhetic shellfish … Show more
“…Hence, commercially available ELISA kits have been successfully tested for the determination and quantification of antimicrobials (e.g., crystal violet, chloramphenicol, gentamicin, fluoroquinolone enrofloxacin, malachite green, metabolites of furaltadone and furazolidone) in fish from aquaculture, to assess illegal use of the compounds (Jester et al, 2014 ; Conti et al, 2015 ). These kits have also proved effective in the detection of toxins [e.g., paralytic shellfish poisoning (PSP), diarrhetic shellfish poisoning, neurotoxic shellfish poisoning, and amnesic shellfish] in shellfish and seafood (Garet et al, 2010 ; Huazhang et al, 2011 ; Eberhart et al, 2013 ; Turner and Goya, 2016 ) and pesticides in fish (Sapozhnikova et al, 2015 ). Still, when ELISA method was compared to a phosphatase 2A inhibition assay, the latter displayed more promising results as a screening tool for diarrhetic shellfish toxins, given the sensitivity and low level of false results (Eberhart et al, 2013 ).…”
Enzymes have been used for the production and processing of fish and seafood for several centuries in an empirical manner. In recent decades, a growing trend toward a rational and controlled application of enzymes for such goals has emerged. Underlying such pattern are, among others, the increasingly wider array of enzyme activities and enzyme sources, improved enzyme formulations, and enhanced requirements for cost-effective and environmentally friendly processes. The better use of enzyme action in fish- and seafood-related application has had a significant impact on fish-related industry. Thus, new products have surfaced, product quality has improved, more sustainable processes have been developed, and innovative and reliable analytical techniques have been implemented. Recent development in these fields are presented and discussed, and prospective developments are suggested.
“…Hence, commercially available ELISA kits have been successfully tested for the determination and quantification of antimicrobials (e.g., crystal violet, chloramphenicol, gentamicin, fluoroquinolone enrofloxacin, malachite green, metabolites of furaltadone and furazolidone) in fish from aquaculture, to assess illegal use of the compounds (Jester et al, 2014 ; Conti et al, 2015 ). These kits have also proved effective in the detection of toxins [e.g., paralytic shellfish poisoning (PSP), diarrhetic shellfish poisoning, neurotoxic shellfish poisoning, and amnesic shellfish] in shellfish and seafood (Garet et al, 2010 ; Huazhang et al, 2011 ; Eberhart et al, 2013 ; Turner and Goya, 2016 ) and pesticides in fish (Sapozhnikova et al, 2015 ). Still, when ELISA method was compared to a phosphatase 2A inhibition assay, the latter displayed more promising results as a screening tool for diarrhetic shellfish toxins, given the sensitivity and low level of false results (Eberhart et al, 2013 ).…”
Enzymes have been used for the production and processing of fish and seafood for several centuries in an empirical manner. In recent decades, a growing trend toward a rational and controlled application of enzymes for such goals has emerged. Underlying such pattern are, among others, the increasingly wider array of enzyme activities and enzyme sources, improved enzyme formulations, and enhanced requirements for cost-effective and environmentally friendly processes. The better use of enzyme action in fish- and seafood-related application has had a significant impact on fish-related industry. Thus, new products have surfaced, product quality has improved, more sustainable processes have been developed, and innovative and reliable analytical techniques have been implemented. Recent development in these fields are presented and discussed, and prospective developments are suggested.
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