Detection of SGI1/PGI1 Elements and Resistance to Extended-Spectrum Cephalosporins in Proteae of Animal Origin in France

Schultz, Eliette; Cloeckaert, Axel; Doublet, Benoît; Madec, Jean‐Yves; Haenni, Marisa

doi:10.3389/fmicb.2017.00032

Cited by 16 publications

(14 citation statements)

References 35 publications

(54 reference statements)

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“…Our data agree with the study in Taiwan which revealed the same prevalence of MDR S. Albany, mediated by SGI1-F in humans and swine, and suggested the clonal dissemination of a SGI1-F-carrying strain in Taiwan (Kuo et al, 2014). In contrast, a Salmonella surveillance study in Scotland during 1990Scotland during -2004 showed differences in the prevalence of SGI1 variants in human and animal isolates, and the independent origin of isolates within each population (Mather et al, 2011 (Schultz, Cloeckaert, Doublet, Madec, & Haenni, 2017). The relationship between animal and human Salmonella isolates regarding SGI1 variants, along with their closely associated PFGE patterns, indicates that food-producing animals may be the source of MDR isolates in humans.…”

Section: Discussionsupporting

confidence: 90%

“…In a similar study, the PFGE analysis of SGI1‐positive serotype Agona isolates demonstrated identical patterns and was clonally related (Doublet, Butaye, et al (), whereas the study of SGI1 containing Proteus spp. isolates exhibited unrelated PFGE patterns except the six SGI1‐V containing isolates (Schultz, Cloeckaert, Doublet, Madec, & Haenni, ). The relationship between animal and human Salmonella isolates regarding SGI1 variants, along with their closely associated PFGE patterns, indicates that food‐producing animals may be the source of MDR isolates in humans.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and characterization of multidrug resistance and variant Salmonella genomic island 1 in Salmonella isolates from cattle, poultry and humans in Iran

Ghoddusi

Fasaei

Salehi

et al. 2019

Zoonoses and Public Health

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Salmonella enterica is a common food‐borne pathogen with occasional multidrug resistance (MDR). Salmonella genomic island (SGI1) is a horizontally transmissible genomic island, containing an MDR gene cluster. All Salmonella serotypes are public health concern, although there is an additional concern associated with those that harbour SGI1. In Iran, there are no data on the presence of SGI1 variants in Salmonella isolates. The present study was conducted to identify MDR‐ and SGI1‐carrying Salmonella strains isolated from various sources and to compare their genetic relatedness between human and animal sources. In total, 242 Salmonella isolates collected from chicken, cattle, and humans from 2008 through 2014 were studied. The isolates were tested for resistance to 14 antimicrobials via the disc diffusion method. They were also tested for the presence of SGI1 variants via PCR, and genetic relatedness was evaluated based on pulsed‐field gel electrophoresis (PFGE). Resistance to at least one antimicrobial agent was observed in 132 (54%) Salmonella isolates (n = 242), while more than 40% of the isolates showed MDR. Based on PCR analysis, eight variants of SGI1, including SGI1, SGI1‐B, SGI1‐C, SGI1‐D, SGI1‐F, SGI1‐I, SGI1‐J and SGI1‐O, were found in both human and animal isolates. Statistical analysis revealed no significant difference in the prevalence of SGI1 variants between human and animal isolates (p > 0.05). Macrorestriction PFGE analysis of the isolates with the same SGI1 variant and resistance patterns revealed genetic relatedness ranging from 70% to 100% among human and animal isolates. According to our review, this is the first documentation of SGI1 in Salmonella isolates in Iran. The presence of similar SGI1 variants in both humans and animals, along with their related PFGE patterns, suggests that food‐producing animals may be a source of MDR Salmonella isolates in Iran.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Prevalence and characterization of multidrug resistance and variant Salmonella genomic island 1 in Salmonella isolates from cattle, poultry and humans in Iran

Ghoddusi

Fasaei

Salehi

et al. 2019

Zoonoses and Public Health

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“…Within veterinary strains of Morganellaceae, bla CTX−M−15 (P. mirabilis) and bla CTX−M−1 (P. rettgeri) have been recently reported. In this study, the bla CTX−M−15 gene was even detected in duplicate, one chromosomally-and the other plasmid-located (non-typable) (Schultz et al, 2017).…”

Section: Ctx-msmentioning

confidence: 58%

Genetics of Acquired Antibiotic Resistance Genes in Proteus spp.

2020

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Proteus spp. are commensal Enterobacterales of the human digestive tract. At the same time, P. mirabilis is commonly involved in urinary tract infections (UTI). P. mirabilis is naturally resistant to several antibiotics including colistin and shows reduced susceptibility to imipenem. However higher levels of resistance to imipenem commonly occur in P. mirabilis isolates consecutively to the loss of porins, reduced expression of penicillin binding proteins (PBPs) PBP1a, PBP2, or acquisition of several antibiotic resistance genes, including carbapenemase genes. In addition, resistance to nonβ-lactams is also frequently reported including molecules used for treating UTI infections (e.g., fluoroquinolones, nitrofurans). Emergence and spread of multidrug resistant P. mirabilis isolates, including those producing ESBLs, AmpC cephalosporinases and carbapenemases, are being more and more frequently reported. This review covers Proteus spp. with a focus on the different genetic mechanisms involved in the acquisition of resistance genes to multiple antibiotic classes turning P. mirabilis into a dreadful pandrug resistant bacteria and resulting in difficult to treat infections.

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“…Unfortunately, conjugation was unsuccessful. In addition, the detection of bla VEB-6 and bla NDM-1 in PGI1-PmPEL and bla VEB-6 -carrying SGI1-V in P. mirabilis strains isolated in France are a serious public health threat (10). Recently, we reported the detection of SGI1-PmABB in the P. mirabilis strain PmTAN59 in the same hospital (6).…”

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confidence: 97%

Emergence of Salmonella Genomic Island 1 Variant SGI1-W in a Clinical Isolate of Providencia stuartii from Egypt

Soliman

Nariya

Shimamoto

2019

Antimicrob Agents Chemother

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M ost of the variants of Salmonella genomic island 1 (SGI1) are multidrug resistance (MDR) DNA elements that can be transferred by horizontal gene transfer (HGT) (1). SGI1 was initially described in Salmonella enterica serovar Typhimurium DT104 (1). It consists of a 27.4-kb backbone (28 open reading frames [ORFs]) with an MDR region consisting of a 15-kb complex class 1 integron of the family In104 (1). The SGI1 variant SGI1-L was detected for the first time in Proteus mirabilis and Morganella morganii strains from Palestine in 2006 and from France in 2017, respectively (2, 3). The 3= end of the chromosomal trmE gene is the SGI1-specific integration site in the SGI1-carrying strains (3, 4). Providencia stuartii is Gram-negative bacterium of the family Morganellaceae commonly associated with urinary tract infections or indwelling urinary catheters in hospitalized and nursing home patients (5). It has intrinsic resistance to polymyxin, tigecycline, aminopenicillin, and first-generation cephalosporins (5). Here, we report the first identification (to our knowledge) of SGI1 in a multidrugresistant clinical isolate of P. stuartii. The strain, designed PsTAN3, was isolated from a wound pus swab from a male patient in a teaching hospital in Tanta City, Egypt, in 2014. PsTAN3 was resistant to aztreonam (MIC, Ն512 g/ml), ciprofloxacin (MIC, Ն16 g/ml), chloramphenicol (MIC, Ն512 g/ml), colistin (MIC, Ն128 g/ml), gentamicin (MIC, Ն128 g/ml), tetracycline (MIC, Ն128 g/ml), streptomycin (MIC, Ն512 g/ml), cefoxitin (MIC, Ն256 g/ml), cefoperazone (MIC, Ն512 g/ml), trimethoprim (MIC, Ն512 g/ml), cefotaxime (MIC, Ն512 g/ml), meropenem (MIC, Ն16 g/ml), and nalidixic acid (MIC, Ն512 g/ml) but sensitive to doripenem (MIC, Յ1 g/ml). PsTAN3 was negative by the modified carbapenem inactivation method (mCIM), indicating the absence of carbapenemase production. PCR and DNA sequencing were used to map the entire SGI1 (for a list of PCR primers, see Table S1 in the supplemental material) and identified variant SGI1-W (6, 7). The multidrug resistance region was 6.54 kb with the genetic arrangement intI1-aadA2-lnuF-qacE⌬1-sul1-orf5-orf6-IS6100 (PsTAN3 class 1 integron GenBank accession no. LC370342) (Fig. 1). SGI1-W was integrated at the 3= end of the trmE gene (Fig. 1). The strain also carried bla TEM-1 , bla DHA-1 , qnrD, and floR genes conferring resistance to third-generation cephalosporins, ciprofloxacin, florfenicol, and chloramphenicol, respectively, and the class 1 integrons aadA2 and lnuF. The MDR region of SGI1 in PsTAN3 was 100% identical to that of SGI1-PmC105 reported in a P. mirabilis isolate from a poultry farm in China (7). Furthermore, the circular form was detected in PsTAN3 after two steps of DNA amplification using primers SGI1circ1 and SGI1circ2 with the expected target size (364 bp) (GenBank accession no. LC370343) (see Fig. S1A in the supplemental material). The sequence of the SGI1-W attP site in PsTAN3 (5=-TTCTGTATCGGGAAGTAA-3=) differs in 1 bp (C¡T) from that of S. enterica DT104 and P. mirabilis (5=-TTCTGTATTGGGAAG...

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Detection of SGI1/PGI1 Elements and Resistance to Extended-Spectrum Cephalosporins in Proteae of Animal Origin in France

Cited by 16 publications

References 35 publications

Prevalence and characterization of multidrug resistance and variant Salmonella genomic island 1 in Salmonella isolates from cattle, poultry and humans in Iran

Prevalence and characterization of multidrug resistance and variant Salmonella genomic island 1 in Salmonella isolates from cattle, poultry and humans in Iran

Genetics of Acquired Antibiotic Resistance Genes in Proteus spp.

Emergence of Salmonella Genomic Island 1 Variant SGI1-W in a Clinical Isolate of Providencia stuartii from Egypt

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