Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental Tissue

Sauerbrei, Beate; Wandernoth, Petra; Kriegsmann, Mark; Casadonte, Rita; Kriegsmann, Katharina; Kriegsmann, Joerg

doi:10.3390/v14030604

Cited by 2 publications

(2 citation statements)

References 41 publications

(59 reference statements)

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“…In fact, only a very limited number of studies combine data about the relationship between SARS-CoV-2 variants and positive placental SARS-CoV-2 tests. Wierz et al [ 28 ], reporting on an isolated case, detected the presence of the Alpha variant in placental tissue using MALDI-TOF technology. Argueta et al [ 29 ] described a cohort of placental samples from mothers positive for SARS-CoV-2 at delivery, where forty-two percent had detectable RNA, and underscored two placentas from mothers infected with the Alpha variant.…”

Section: Discussionmentioning

confidence: 99%

Placental Infection Associated with SARS-CoV-2 Wildtype Variant and Variants of Concern

Medel-Martinez,

Paules,

Peran

et al. 2023

Viruses

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The original SARS-CoV-2 lineages have been replaced by successive variants of concern (VOCs) over time. The aim of this study was to perform an assessment of the placental infection by SARS-CoV-2 according to the predominant variant at the moment of COVID-19 diagnosis. This was a prospective study of SARS-CoV-2-positive pregnant women between March 2020 and March 2022. The population was divided into pregnancies affected by COVID-19 disease during 2020 (Pre-VOC group) and pregnancies affected after December 2020 by SARS-CoV-2 variants of concern (VOC group). The presence of virus was assessed by RT-PCR, and the viral variant was determined by whole genome sequencing. A total of 104 placentas were examined, among which 54 cases belonged to the Pre-VOC group and 50 cases belonged to the VOC group. Sixteen positive placental RT-PCR tests for SARS-CoV-2 were reported. The NGS analysis confirmed the SARS-CoV-2 lineage in placenta tissue. All samples corresponded to the Pre-VOC group, whereas no placental presence of SARS-CoV-2 was detected in the VOC group (16, 29.6% vs. 0, 0.0% p = 0.000). Preterm birth (9, 16.7% vs. 2, 4%; p = 0.036) and hypertensive disorders of pregnancy (14, 25.9% vs. 3, 6%; p = 0.003) were more frequent in the Pre-VOC group than in the VOC group. Finally, the VOC group was composed of 23 unvaccinated and 27 vaccinated pregnant women; no differences were observed in the sub-analysis focused on vaccination status. In summary, SARS-CoV-2-positive placentas were observed only in pregnancies infected by SARS-CoV-2 wildtype. Thus, placental SARS-CoV-2 presence could be influenced by SARS-CoV-2 variants, infection timing, or vaccination status. According to our data, the current risk of SARS-CoV-2 placental infection after maternal COVID disease during pregnancy should be updated.

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Section: Discussionmentioning

confidence: 99%

Placental Infection Associated with SARS-CoV-2 Wildtype Variant and Variants of Concern

Medel-Martinez,

Paules,

Peran

et al. 2023

Viruses

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“…Furthermore, the technology may clarify microbial resistance mechanisms [5,6]. Numerous reports have been published identifying bacteria, fungi [6], and various viruses [7,8].…”

Section: Mass Spectrometry In Medicinementioning

confidence: 99%

Perspective Chapter: Predictive Genomics

Kriegsmann,

Cirovic,

Casadonte

et al. 2024

Electron Microscopes, Spectroscopy and Their Applications

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Predictive genomics can support treatment decisions by giving people the chance to act in time to prevent serious illness. Tests based on single nucleotide polymorphism (SNP) can be analyzed by various methods. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry technology detects genetic variants based on their individual mass. Standardized workflow, automation, sensitivity, quick turnaround time, and reliability are the main advantages of the MALDI-TOF use in molecular analysis. Beside pharmacogenetics, SNP variation plays a role in various fields of medicine. In the present article importance of various SNPs for nutrigenetics is presented. Especially, various aspects of fat metabolism, vitamin metabolism, and intolerances were discussed.

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Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) including Variant Analysis by Mass Spectrometry in Placental Tissue

Cited by 2 publications

References 41 publications

Placental Infection Associated with SARS-CoV-2 Wildtype Variant and Variants of Concern

Placental Infection Associated with SARS-CoV-2 Wildtype Variant and Variants of Concern

Perspective Chapter: Predictive Genomics

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