Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in the Pleural Fluid

Baek, Moon Seong; Kim, Won-Young; Lee, Kyoung Ju; Noh, Chang Suk

doi:10.3947/ic.2020.0045

Cited by 6 publications

(3 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite the theoretical risk of contamination, only few case reports con rmed the presence of SARS-CoV-2 in abdominal uids (10)(11)(12), while some researchers also reported the viral involvement of pleural uids (13)(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Swab test in biological fluids as predictor of COVID-19 transmission risk during surgery: A prospective cross-sectional study from an Italian COVID center

Fabbri

Pesce

Ussia

et al. 2021

Preprint

View full text Add to dashboard Cite

Background. The contamination of body fluids by Severe Acute Respiratory Syndrome Coronavirus 2 during surgery is current matter of debate in the scientific literature concerning CoronaVIrus Disease 2019. Surgical guidelines were published during the first wave of the COVID-19 pandemic and recommended to avoid laparoscopic surgery as much as possible, in fear that the chimney effect of high flow intraperitoneal gas escape during, and after, the procedure would increase the risk of viral transmission.Aim. The aim of this study was to evaluate the possibility of SARS-CoV-2 transmission during surgery by searching for viral RNA in serial samplings of biological liquids.Methods. This is a single center prospective cross-sectional study. We used a real-time reverse transcriptase (RT) polymerase chain reaction (PCR) test to perform swab tests for the qualitative detection of nucleic acid from SARS-CoV-2 in abdominal fluids, during emergency surgery and on the first post-operative day. In the case of thoracic surgery, we performed a swab test of pleural fluids during chest drainage placement as well as on the first post-operative day. Results. A total of 20 samples were obtained: 5 from pleural fluids, 13 from peritoneal fluids and two from biliary fluid. All 20 swabs performed from biological fluids resulted negative for SARS-CoV-2 RNA detection.Conclusion. To date, there is no scientific evidence of possible contagion by laparoscopic aerosolization of SARS-CoV-2, neither is certain whether the virus is effectively present in biological fluids.

show abstract

Section: Introductionmentioning

confidence: 99%

Swab test in biological fluids as predictor of COVID-19 transmission risk during surgery: A prospective cross-sectional study from an Italian COVID center

Fabbri

Pesce

Ussia

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Relevant incidence of long-term consequences poses a significant burden on health sector. While substantial work has previously been done in the understanding of COVID-pneumonia, little is known about the pleural damage [ 5 , 6 ]. End-stage disease of various carcinoma types, especially adenocarcinoma of the lung, mamma, or ovaries, can lead to a diffuse metastatic spread of the pleura, so called pleura carcinosis.…”

Section: Introductionmentioning

confidence: 99%

Novel 3D organotypic co-culture model of pleura

et al. 2022

View full text Add to dashboard Cite

Pleural mesothelial cells are the predominant cell type in the pleural cavity, but their role in the pathogenesis of pleural diseases needs to be further elucidated. 3D organotypic models are an encouraging approach for an in vivo understanding of molecular disease development. The aim of the present study was to develop a 3D organotypic model of the pleural mesothelium. Specimens of human pleura parietalis were obtained from patients undergoing surgery at the University Hospital Leipzig, Germany. 3D co-culture model of pleura was established from human pleural mesothelial cells and fibroblasts. The model was compared to human pleura tissue by phase-contrast and light microscopy, immunochemistry and -fluorescence as well as solute permeation test. Histological assessment of the 3D co-culture model displayed the presence of both cell types mimicking the morphology of the human pleura. Vimentin and Cytokeratin, PHD1 showed a similar expression pattern in pleural biopsies and 3D model. Expression of Ki-67 indicates the presence of proliferating cells. Tight junctional marker ZO-1 was found localized at contact zones between mesothelial cells. Each of these markers were expressed in both the 3D co-culture model and human biopsies. Permeability of 3D organotypic co-culture model of pleura was found to be higher for 70 kDa-Dextran and no significant difference was seen in the permeability for small dextran (4 kDa). In summary, the presented 3D organoid of pleura functions as a robust assay for pleural research serving as a precise reproduction of the in vivo morphology and microenvironment.

show abstract

“…Un estudio previo de líquido pleural reportó la presencia de células mesoteliales con grandes y múltiples núcleos, (14) consistente con un efecto citopático de infección por virus . Aunque no es una metodología de uso común y recomendada por la complejidad en su proceso, se ha reportado la presencia de SARS-CoV-2 a través de un ensayo de RT-PCR en (12,15) tiempo real del líquido pleural .…”

Section: Introductionunclassified

Hallazgos citoquímicos en líquido pleural de pacientes con COVID-19, atendidos en un servicio de emergencia

Quispe-Aranda

Arce-Silva²,

Neyra-Vera³

et al. 2021

Rev. Cuerpo Med. HNAAA

View full text Add to dashboard Cite

Objetivo: El presente trabajo tuvo como propósito describir las características físicas, citológicas y bioquímicas, así como clasificar los líquidos pleurales de pacientes con COVID-19 como exudados o trasudados. Material y Métodos: Se realizó un estudio observacional, transversal descriptivo. Se recopiló datos del examen citoquímico de muestras de líquido pleural procesadas entre los meses de abril a setiembre del 2020, en el laboratorio del Centro de Emergencias de Lima Metropolitana (CELIM). Resultados: Durante el periodo de estudio un total de 81 muestras de líquido pleural de pacientes con COVID-19 fueron evaluadas, de estos, 26 cumplieron con nuestros criterios de elegibilidad. Catorce líquidos (54%), tuvieron un color rojo y 18 (69%) tuvieron un aspecto turbio. Los líquidos pleurales exudativos fueron la mayoría (n = 17), las proteínas totales presentaron una media de 3.3 gr/dl (DE: 1.5), LDH una mediana de 544 U/L (RIC: 262-2016), el recuento leucocitario tuvo una mediana de 610 leucocitos/ul (RIC: 180-968) y el recuento diferencial de polimorfonucleares una mediana 29% (RIC: 15-60). En cuanto a las características clínicas, del total de exudados el 41% correspondían a neoplasias y el 23% a neumonías. Mientras que, del total de trasudados (n=9), 45% provenían de pacientes con neoplasias. Conclusiones: En nuestro estudio observamos que la mayoría de las muestras de líquido pleural de pacientes con COVID-19 fueron clasificadas como exudados. Estas presentaron un bajo porcentaje diferencial leucocitario de polimorfonucleares. Los valores de los parámetros bioquímicos tales como proteínas totales y LDH se relacionaron adecuadamente con la clasificación de exudados.

show abstract

Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in the Pleural Fluid

Cited by 6 publications

References 5 publications

Swab test in biological fluids as predictor of COVID-19 transmission risk during surgery: A prospective cross-sectional study from an Italian COVID center

Swab test in biological fluids as predictor of COVID-19 transmission risk during surgery: A prospective cross-sectional study from an Italian COVID center

Novel 3D organotypic co-culture model of pleura

Hallazgos citoquímicos en líquido pleural de pacientes con COVID-19, atendidos en un servicio de emergencia

Contact Info

Product

Resources

About