Detection of several strains of the bacterium-like organism of citrus greening disease by DNA probes

Villechanoux, S.; Garnier, M.; Renaudin, Joël; Bové, Joseph M.

doi:10.1007/bf01570903

Cited by 75 publications

(59 citation statements)

References 12 publications

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“…Total DNA was extracted from periwinkle or sweet orange leaf midribs collected on healthy plants or plants infected with one of the three liberibacters. The DNA was dot-blotted onto nylon N+ membranes according to Villechanoux et al (1992). Two probes were used.…”

Section: Methodsmentioning

confidence: 99%

“…The probes were purified on Promega columns before use. Hybridization was according to Villechanoux et al (1992).…”

Section: Methodsmentioning

confidence: 99%

“…L. asiaticus' (Villechanoux et al, 1992(Villechanoux et al, , 1993. Next, DNA from periwinkle plants infected with the Nelspruit strain (South Africa) of 'Ca.…”

Section: Introductionmentioning

confidence: 99%

“…L. africanus' (Planet et al, 1995). In-2.6 and AS-1.7 have been used as specific hybridization probes for the detection of the Asian and African liberibacter strains, respectively (Villechanoux et al, 1992;Hocquellet et al, 1997). They have served to design PCR primers rplA2 and rplJ5 for detection of liberibacters by amplification of ribosomal protein genes (Hocquellet et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…L. asiaticus' (GenBank accession no. EU078703), the sequences of an Indian strain (M94319; Villechanoux et al, 1992Villechanoux et al, , 1993 and a Japanese strain (AY342001; Okuda et al, 2005) being already known. Finally, the b operon sequence of 'Ca.…”

Section: Introductionmentioning

confidence: 99%

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The tufB-secE-nusG-rplKAJL-rpoB gene cluster of the liberibacters: sequence comparisons, phylogeny and speciation

Teixeira¹,

Eveillard²,

Sirand‐Pugnet³

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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The rplKAJL-rpoBC operon or b operon is a classic bacterial gene cluster, which codes for proteins K, A, J and L of the large ribosomal subunit, as well as proteins B (b subunit) and C (b9 subunit) of RNA polymerase. In the early 1990s, the operon was obtained as a 2.6 kbp DNA fragment (In-2.6) by random cloning of DNA from periwinkle plants infected with the Poona (India) strain of the huanglongbing agent, later named 'Candidatus (Ca.) Liberibacter asiaticus'. DNA from periwinkle plants infected with the Nelspruit strain (South Africa) of 'Ca. L. africanus' was amplified with a primer pair designed from In-2.6 and yielded, after cloning and sequencing, a 1.7 kbp DNA fragment (AS-1.7) of the b operon of 'Ca. L. africanus'. The b operon of the American liberibacter, as well as the three upstream genes (tufB, secE, nusG), have now also been obtained by the technique of chromosome walking and extend over 4673 bp, comprising the following genes: tufB, secE, nusG, rplK, rplA, rplJ, rplL and rpoB. The sequence of the b operon was also determined for a Brazilian strain of 'Ca. L. asiaticus', from nusG to rpoB (3025 bp), and was found to share 99 % identity with the corresponding b operon sequences of an Indian and a Japanese strain. Finally, the b operon sequence of 'Ca. L. africanus' was extended from 1673 bp (rplA to rpoB) to 3013 bp (nusG to rpoB), making it possible to compare the b operon sequences of the African, Asian and American liberibacters over a length of~3000 bp, from nusG to rpoB. While 'Ca. L. africanus' and 'Ca. L. asiaticus' shared 81.2 % sequence identity, the percentage for 'Ca. L. americanus' and 'Ca. L. africanus' was only 72.2 %, and identity for 'Ca. L. americanus' and 'Ca. L. asiaticus' was only 71.4 %. The~3000 bp nusG-rpoB sequence was also used to construct a phylogenetic tree, and this tree was found to be identical to the known 16S rRNA gene sequence-based tree. These results confirm earlier findings that 'Ca. L. americanus' is a distinct liberibacter, more distantly related to 'Ca. L. africanus' and 'Ca. L. asiaticus' than 'Ca. L. africanus' is to 'Ca. L. asiaticus'. The dates of speciation have also been estimated.

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Section: Methodsmentioning

confidence: 99%

“…The probes were purified on Promega columns before use. Hybridization was according to Villechanoux et al (1992).…”

Section: Methodsmentioning

confidence: 99%

“…L. asiaticus' (Villechanoux et al, 1992(Villechanoux et al, , 1993. Next, DNA from periwinkle plants infected with the Nelspruit strain (South Africa) of 'Ca.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The tufB-secE-nusG-rplKAJL-rpoB gene cluster of the liberibacters: sequence comparisons, phylogeny and speciation

Teixeira¹,

Eveillard²,

Sirand‐Pugnet³

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Liberibacter

Garnier¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Li.be.ri' bac.ter . L. n. liber phloem; Gr. n. bakterion a small rod; N.L. masc. n. Liberibacter rod in the phloem. Copyright © 2015 John Wiley & Sons, Ltd. Proteobacteria / Alphaproteobacteria / Rhizobiales / Phyllobacteriaceae / Liberibacter Filamentous bacteria occurring in the phloem sieve tubes of plants (Fig. 146). Round forms are also observed but have been shown to correspond to degenerating bacteria. The bacteria are also present in the hemolymph and salivary glands of insect vectors (Psyllidae) responsible for the transmission. Like most phloem‐restricted bacteria, Candidatus Liberibacter spp. have resisted in vitro cultivation (Garnier and Bové, 1993). The characterized species originated from sweet orange ( Citrus sinensis ). The original description is based on bacteria present in the phloem sieve tubes of sweet orange trees affected by huanglongbing (ex. greening disease) in Poona (India) and Nelspruit (South Africa). Type species : Candidatus Liberibacter asiaticus Jagoueix, Bové and Garnier 1994, 385 ( Candidatus Liberobacter asiaticum (sic) Jagoueix, Bové and Garnier 1994, 385.)

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Long PCR Is a Sensitive Method for Detecting Liberobacter asiaticum in Parasitoids Undergoing Risk Assessment in Quarantine

2001

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Detection of several strains of the bacterium-like organism of citrus greening disease by DNA probes

Cited by 75 publications

References 12 publications

The tufB-secE-nusG-rplKAJL-rpoB gene cluster of the liberibacters: sequence comparisons, phylogeny and speciation

The tufB-secE-nusG-rplKAJL-rpoB gene cluster of the liberibacters: sequence comparisons, phylogeny and speciation

Liberibacter

Long PCR Is a Sensitive Method for Detecting Liberobacter asiaticum in Parasitoids Undergoing Risk Assessment in Quarantine

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