Detection of serine proteinase inhibitors in human cornified cells

Hibino, Toshihiko; Izaki, Seiichi; Izaki, Masakatsu

doi:10.1016/0006-291x(81)91841-6

Cited by 23 publications

(4 citation statements)

References 19 publications

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“…Overexpression or activation of these proteinases and speci®c inhibitors are possibly responsible, at least in part, for the disease activity in psoriasis. Normal epidermis contains three detectable proteinase inhibitors: plasminogen activator inhibitor 2 (Hibino et al, 1988b), a trypsin inhibitor (Hibino et al, 1981), and a low molecular mass cysteine proteinase inhibitor (cystatin) (Ja Èrvinen, 1978;Takeda et al, 1986).…”

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confidence: 99%

Overexpression of Serpin Squamous Cell Carcinoma Antigens in Psoriatic Skin

Takeda

Higuchi

Takahashi

et al. 2002

Journal of Investigative Dermatology

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Squamous cell carcinoma antigen belongs to the serpin family and is used for the diagnosis and management of squamous cell carcinoma. We investigated the involvement of squamous cell carcinoma antigen in psoriasis, as it is always detected in the sera of patients with psoriasis. Squamous cell carcinoma antigen localization in psoriatic epidermis varied depending on its concentration in the patient's sera. When its level was low in serum, weak and scattered staining was observed in the granular layer. With a high concentration of squamous cell carcinoma antigen, strong staining through the suprabasal to granular layer and condensed staining around the plasma membrane or intracellular space was detected in the affected epidermis. Interestingly, squamous cell carcinoma antigen was abundant in nuclei of the granular layer cells and elongated rete ridges. Immunoelectron microscopy confirmed the localization of squamous cell carcinoma antigen in the nuclei as well as in the periphery of the cell membrane. A cDNA library was constructed from psoriatic epidermis and both clones, SCCA1 and SCCA2, were obtained. Attempts to raise specific antibodies or to prepare cRNA probes for SCCA1 and SCCA2 were unsuccessful because of their nearly identical structures. A primer pair from each reactive site sequence enabled us to give a distinctive product for SCCA1 and SCCA2 by reverse transcription polymerase chain reaction. Analysis using these primers demonstrated that the SCCA2 transcript was specifically expressed in psoriatic skin tissues. Our results suggest that overexpression of squamous cell carcinoma antigens is associated with the disease activity of psoriasis.

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Overexpression of Serpin Squamous Cell Carcinoma Antigens in Psoriatic Skin

Takeda

Higuchi

Takahashi

et al. 2002

Journal of Investigative Dermatology

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“…Therefore, the second possibility arises that some release of active substances may occur after such membrane changes. Hibino et al (17) extracted serine proteinase inhibitor from human cornified cell. Presumably, heat treatment may cause the release of these inhibitors, resulting in suppression of complement activation.…”

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confidence: 99%

Reduction of Chemotactic Activities in Psoriatic Lesions by Hyperthermia Treatments

Isoda

Oosaki

Yasumoto

et al. 1984

The Journal of Dermatology

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The effect of the local heat treatment on the chemotactic activity in psoriatic scales was studied by using a modified Boyden chamber method. Hyperthermic treatments were given to psoriasis plaques and scales were taken from these plaques before and after treatment. The psoriasis improved over a two week period of these treatments, but plaques were still present and scaling at that time. Treatment with local hyperthermia significantly reduced the activity for polymorphonuclear leukocytes in psoriatic scales (p<0.05). This result suggests that the inhibition of chemotactic activity may be involved in the action mechanism of hyperthermia in psoriatic lesions.

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“…Measurements were done in duplicate in a method previously described (9,15). In order to detect enzymatic activity, 0.1 or 0.05 ml of the sample solution was mixed with 0.85-0.90 ml (total volume, 0.95 ml) of 0.1 M Tris-HCl, pH 8.0, in 1 ml semimicrocuvette set at 37° C in a water-jacketed cell holder in a spectrophotometer, Hitachi 200-20, equipped with an automatic recorder.…”

Section: Spectrophotometric Assay %mentioning

confidence: 99%

Plasminogen Activator and Plasminogen Activator Inhibitor Associated with Granulomatous Inflammation: A Study with Murine Leprosy

et al. 1984

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SummaryPlasminogen activator that is associated with the development of hypersensitivity granulomas (gPA) was partially purified from a saline soluble fraction of murine lepromas elicited in “resistant” mice, C57BL/6N. The gPA was shown to consist of two subspecies (23,000 and 48,000 in molecular weight) with essentially identical enzymologic properties. The gPA was found to be a relatively heat stable weakly alkaline serine proteinase with trypsin-like characteristics in the specificity for synthetic substrates and proteinase inhibitors. It showed a high affinity for H- D-Ile-Pro-Arg-pNA (Km = 1.4 × 10-4 M) H-D-Val-Leu-Lys- pNA (Km = 5.2 × 10-4 M), and L-pyroGlu-Gly-Arg-pNA (Km = 9.3 × 10-4 M). The gPA did not demonstrate antigenic cross reaction with urokinase-type or tissue-type plasminogen activator.Two distinct enzymatic regulators of the gPA were also demonstrated in the saline soluble fraction of the hypersensitivity granulomas. The gPA and its regulation are assumed to be correlated with macrophage activation in the hypersensitivity granulomas

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Detection of serine proteinase inhibitors in human cornified cells

Cited by 23 publications

References 19 publications

Overexpression of Serpin Squamous Cell Carcinoma Antigens in Psoriatic Skin

Overexpression of Serpin Squamous Cell Carcinoma Antigens in Psoriatic Skin

Reduction of Chemotactic Activities in Psoriatic Lesions by Hyperthermia Treatments

Plasminogen Activator and Plasminogen Activator Inhibitor Associated with Granulomatous Inflammation: A Study with Murine Leprosy

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