Detection of Sepsis in Preterm Infants by Fecal Volatile Organic Compounds Analysis

Berkhout, Daniel J. C.; Niemarkt, Hendrik J.; Buijck, Martin; Weissenbruch, Mirjam M. van; Brinkman, Paul; Benninga, Marc A.; Kaam, Anton H. van; Kramer, Boris W.; Andriessen, Peter; Boer, Nanne K. H. de; Meij, Tim G. J. de

doi:10.1097/mpg.0000000000001471

Cited by 43 publications

(46 citation statements)

References 43 publications

(76 reference statements)

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“…These characteristics have been used in previous studies on the potential of VOC profiling in the (early) detection of colorectal cancer, pediatric inflammatory bowel disease, necrotizing enterocolitis and late-onset sepsis, using the same eNose device as in the present study. Applying these baseline characteristics VOC analysis allowed for discrimination between these diseases and healthy controls [7,8,9,21]. Per experiment, only the variable of interest changed according to the protocol, all other variables retained the same, corresponding the baseline characteristics.…”

Section: Methodsmentioning

confidence: 99%

Effects of Sampling Conditions and Environmental Factors on Fecal Volatile Organic Compound Analysis by an Electronic Nose Device

Berkhout

Benninga

Stein

et al. 2016

Sensors

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Prior to implementation of volatile organic compound (VOC) analysis in clinical practice, substantial challenges, including methodological, biological and analytical difficulties are faced. The aim of this study was to evaluate the influence of several sampling conditions and environmental factors on fecal VOC profiles, analyzed by an electronic nose (eNose). Effects of fecal sample mass, water content, duration of storage at room temperature, fecal sample temperature, number of freeze–thaw cycles and effect of sampling method (rectal swabs vs. fecal samples) on VOC profiles were assessed by analysis of totally 725 fecal samples by means of an eNose (Cyranose320®). Furthermore, fecal VOC profiles of totally 1285 fecal samples from 71 infants born at three different hospitals were compared to assess the influence of center of origin on VOC outcome. We observed that all analyzed variables significantly influenced fecal VOC composition. It was feasible to capture a VOC profile using rectal swabs, although this differed significantly from fecal VOC profiles of similar subjects. In addition, 1285 fecal VOC-profiles could significantly be discriminated based on center of birth. In conclusion, standardization of methodology is necessary before fecal VOC analysis can live up to its potential as diagnostic tool in clinical practice.

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Section: Methodsmentioning

confidence: 99%

Effects of Sampling Conditions and Environmental Factors on Fecal Volatile Organic Compound Analysis by an Electronic Nose Device

Berkhout

Benninga

Stein

et al. 2016

Sensors

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“…The analysis of fecal VOCs by eNose could discriminate separate sepsis cases ( n = 36) from controls ( n = 40) up to 3 days preceding disease onset [62]. Since the causative agent of LOS is often abundantly present in the days preceding disease onset and each bacterial species produces a unique VOC fingerprint, we suggest that fecal VOCs may allow for the preclinical detection of pathogens at the species level [33].…”

Section: Microbiota-based Diagnosis Of Necmentioning

confidence: 99%

Necrotizing Enterocolitis, Gut Microbiota, and Brain Development: Role of the Brain-Gut Axis

et al. 2019

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Necrotizing enterocolitis (NEC) is a relatively common disease in very-low-birth-weight infants and is associated with high mortality and morbidity. In survivors, neurodevelopmental impairment is frequently seen. The exact etiology remains largely to be elucidated, but microbiota are considered to play a major role in the development of NEC. Furthermore, emerging evidence exists that the microbiota is also of importance in brain function and development. Therefore, microbiota characterization has not only potential as a diagnostic or even preventive tool to predict NEC, but may also serve as a biomarker to monitor and possibly even as a target to manipulate brain development. Analysis of fecal volatile organic compounds, which shape the volatile metabolome and reflect microbiota function and host interaction, has been shown to be of interest in the diagnosis of NEC and late-onset sepsis. In this review, we discuss evidence of the role of the complex interplay between microbiota, NEC, and brain development, including the brain-gut axis in preterm infants.

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“…material, see www.karger.com/doi/10.1159/000489677). In that study, potential diagnostic biomarkers for NEC and sepsis are investigated [11,12]. None of the participating NICUs administered probiotics routinely.…”

Section: Patientsmentioning

confidence: 99%

Risk Factors for Necrotizing Enterocolitis: A Prospective Multicenter Case-Control Study

et al. 2018

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Background: The identification of independent clinical risk factors for necrotizing enterocolitis (NEC) may contribute to early selection of infants at risk, allowing for the development of targeted strategies aimed at the prevention of NEC. Objective: The objective of this study was to identify independent risk factors contributing to the development of NEC in a large multicenter cohort. Methods: This prospective cohort study was performed in 9 neonatal intensive care units. Infants born at a gestational age ≤30 weeks were included. Demographic and clinical data were collected daily until day 28 postnatally. Factors predictive of the development of NEC were identified using univariate and multivariable analyses in a 1: 5 matched case-control cohort. Results: In total, 843 infants (56 NEC cases) were included in this study. In the case-control cohort, univariate analysis identified sepsis prior to the onset of NEC and formula feeding to be associated with an increased risk of developing NEC, whereas the administration of antibiotics directly postpartum was inversely associated with NEC. In a multivariable logistic regression model, enteral feeding type and the number of days parenterally fed remained statistically significantly associated with NEC, whereas the administration of antibiotics directly after birth was associated with a lower risk of developing NEC. Conclusions: Formula feeding and prolonged (duration of) parenteral feeding were associated with an increased risk of NEC. Contrary to expectations, the initiation of treatment with antibiotics within 24 h after birth was inversely associated with NEC.

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Detection of Sepsis in Preterm Infants by Fecal Volatile Organic Compounds Analysis

Cited by 43 publications

References 43 publications

Effects of Sampling Conditions and Environmental Factors on Fecal Volatile Organic Compound Analysis by an Electronic Nose Device

Effects of Sampling Conditions and Environmental Factors on Fecal Volatile Organic Compound Analysis by an Electronic Nose Device

Necrotizing Enterocolitis, Gut Microbiota, and Brain Development: Role of the Brain-Gut Axis

Risk Factors for Necrotizing Enterocolitis: A Prospective Multicenter Case-Control Study

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