Detection of sentinel lymph node metastases in cervical cancer: Assessment of KRT19 mRNA in the one-step nucleic acid amplification (OSNA) method

Okamoto, Satoshi; Niikura, Hitoshi; Nakabayashi, Kadzuki; Hiyama, Kayo; Matoda, Maki; Takeshima, Nobuhiro; Watanabe, Mika; Nagase, Satoru; Otsuki, Takeo; Yaegashi, Nobuo

doi:10.1016/j.ygyno.2013.06.027

Cited by 53 publications

(29 citation statements)

References 39 publications

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“…It has been reported that miR‐492 could originate from the coding sequence of the keratin 19 (KRT19) gene and could be noticeably upregulated in the presence of activated p53 . Previous studies showed that KRT19 mRNA was expressed in normal lymph nodes and was higher in the metastatic lymph nodes than non‐metastatic lymph nodes . Our study showed no contradiction to the published data and indicated miR‐492 as a potentially suitable marker for metastatic lymph node detection.…”

Section: Discussioncontrasting

confidence: 46%

“…30 Previous studies showed that KRT19 mRNA was expressed in normal lymph nodes and was higher in the | 41 metastatic lymph nodes than non-metastatic lymph nodes. 31,32 Our study showed no contradiction to the published data and indicated miR-492 as a potentially suitable marker for metastatic lymph node detection. Furthermore, our study provided the clinical evidence implying the importance of miR-492 in predicting a high risk of treatment failure in advanced cervical cancer patients.…”

Section: Clinical Significance Of Mir-492 In Advanced Cervical Cancersupporting

confidence: 41%

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MicroRNA‐492 overexpression involves in cell proliferation, migration, and radiotherapy response of cervical squamous cell carcinomas

Liu

Huang

et al. 2017

Molecular Carcinogenesis

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MicroRNAs (miRNAs) are small non-coding RNA that target protein-coding mRNAs at the post-transcriptional level. The aim of this study was to define the role of miR-492 in cervical squamous cell carcinomas. After microRNA profiling and comparison, we firstly detected miR-492 expression in 104 tumor tissues biopsies derived from advanced staged (FIGO IIB-IIIB) cervical squamous cell carcinoma patients before receiving concomitant chemoradiotherapy and found miR-492 expression was significantly higher in the specimens that were sensitive to concomitant chemoradiotherapy, as compared with insensitive cancer specimens (P < 0.05). Moreover, higher expression of miR-492 was associated with pelvic lymph node metastasis (LNM) (P < 0.05). Further studies illustrated ectopic miR-492 overexpression in SiHa cells promoted cell proliferation, migration, and enhanced the sensitivity of cervical cancer cells to irradiation by promoting apoptosis. In addition, we identified TIMP2 as a direct miR-492 target, which has been shown to be critical in modulating cancer cell migration and invasion. We also confirmed that miR-492 expression levels in positive pelvic LNM were much higher than negative LNM and miR-492 played a vital role in pelvic lymph node metastasis via regulating miR-492/TIMP2/MMP10 axis. In particular, miR-492 was correlated with prognosis in the subgroup of patients with negative pelvic LNM (P < 0.05) and had a promising value in predicting treatment response in the subgroup of patients with positive pelvic LNM (an AUC of 85%, 75.00% specificity, and 95.24% sensitivity). Taken together, the results suggested that miR-492 may serve as a potential biomarker for cervical cancer treatment and prognosis.

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Section: Discussioncontrasting

confidence: 46%

Section: Clinical Significance Of Mir-492 In Advanced Cervical Cancersupporting

confidence: 41%

MicroRNA‐492 overexpression involves in cell proliferation, migration, and radiotherapy response of cervical squamous cell carcinomas

Liu

Huang

et al. 2017

Molecular Carcinogenesis

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“…Van Trappen et al [32] 19 mRNA was positive in 44% of histologically uninvolved lymph nodes in cervical cancer patients. Okamoto et al [33] examined the sensitivity of the 1-step nucleic acid amplification (OSNA) assay to detect cytokeratin 19 mRNA in SLNs. The OSNA assay showed 96.2% agreement with 2-mm slice histopathological examination.…”

Section: Discussionmentioning

confidence: 99%

Value of Intraoperative Cytological and Pathological Sentinel Lymph Node Diagnosis in Fertility-Sparing Trachelectomy for Early-Stage Cervical Cancer

et al. 2017

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Background and Objectives: Trachelectomy, a fertility-sparing surgery for early-stage cervical cancer, can be performed only when there is no extrauterine extension present. Therefore, identifying the sentinel lymph nodes (SLNs) and using them to obtain an intraoperative pathologic diagnosis can provide information on the feasibility and safety of trachelectomy. Our aim was to assess the value of an intraoperative SLN diagnosis. Methods: We retrospectively analyzed the accuracy of intraoperative imprint cytology and frozen-section examination in 201 patients at our institution in whom trachelectomy was planned. Results: All patients could be evaluated for SLNs; a total of 610 SLNs were analyzed. Although the specificity of both imprint cytology and frozen-section examination was 100.0%, the sensitivity was only 58.6 and 65.5%, respectively. The diagnostic sensitivity was higher in 2-mm slices along the short axis than on bisection along the longitudinal axis. Imprint cytology correctly diagnosed 2 patients who had false-negative results on frozen section. The nature of the metastatic foci that caused an intraoperative false-negative diagnosis was either micrometastasis or isolated tumor cells. Conclusions: The accuracy of intraoperative SLN diagnosis requires improvement, especially when small metastatic foci are present.

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“…3 Several groups recently demonstrated that the detection of sentinel lymph nodes was a feasible and promising technique in patients with uterine cervical cancer. 4,5 However, the detection of sentinel lymph nodes requires an injection of technetium or blue dye and frozen section analysis. These techniques cannot be performed everywhere.…”

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confidence: 99%

Can Pelvic Lymphadenectomy be Omitted in Stage IA2 to IIB Uterine Cervical Cancer?

Togami

Kitajima

Yanazume

et al. 2014

Int J Gynecol Cancer

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Detection of sentinel lymph node metastases in cervical cancer: Assessment of KRT19 mRNA in the one-step nucleic acid amplification (OSNA) method

Abstract: Our results indicated that the KRT19 mRNA OSNA assay can detect LN metastases as accurately as two-mm interval histopathological examination and thus may be an effective additional or alternative method for a rapid intra-operative examination of SLNs in cervical cancer.

Cited by 53 publications

References 39 publications

MicroRNA‐492 overexpression involves in cell proliferation, migration, and radiotherapy response of cervical squamous cell carcinomas

MicroRNA‐492 overexpression involves in cell proliferation, migration, and radiotherapy response of cervical squamous cell carcinomas

Value of Intraoperative Cytological and Pathological Sentinel Lymph Node Diagnosis in Fertility-Sparing Trachelectomy for Early-Stage Cervical Cancer

Can Pelvic Lymphadenectomy be Omitted in Stage IA2 to IIB Uterine Cervical Cancer?

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