Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein

Silva, Patrícia Bento da; Silva, Jaqueline da; Rodrigues, Mosar Corrêa; Vieira, Julia Augusto; Andrade, Ikaro Alves de; Nagata, Tatsuya; Santos, Alexandre Silva; Silva, S. W. da; Rocha, Márcia Cristina Oliveira da; Báo, Sônia Nair; Moraes-Vieira, Pedro M.; Proença-Módena, José Luiz; Angelim, Monara Kaélle Sérvulo Cruz; Souza, Gabriela Fabiano de; Muraro, Stéfanie Primon; Barros, André Luís Branco de; Oliveira, Maria Olivia dos Santos; Ribeiro‐Dias, Fátima; Machado, Giovanna; Fessel, Melissa Regina; Chudzinski-Tavassi, Ana Marisa; Ronconi, Célia M.; Gonçalves, Débora; Curi, Rui; Oliveira, Osvaldo N.; Azevedo, Ricardo Bentes

doi:10.1016/j.talanta.2022.123355

Cited by 16 publications

(15 citation statements)

References 41 publications

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“…The peak appearance at 897 cm −1 was associated with the Ar–H ring deformation vibration of loaded IR783. 36–41 Besides, the fluorescence spectra of BIL were recorded, in which the fluorescence intensity at 810 nm ( λ ex = 760 nm) is proportional to the concentration of IR783 in this concentration range.…”

Section: Resultsmentioning

confidence: 99%

Controlled release of nitric oxide for enhanced tumor drug delivery and reduction of thrombosis risk

et al. 2022

RSC Adv.

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Section: Resultsmentioning

confidence: 99%

Controlled release of nitric oxide for enhanced tumor drug delivery and reduction of thrombosis risk

et al. 2022

RSC Adv.

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“…The dynamic light scattering (DLS) technique can be applied for the determination of the SARS-CoV-2 antigen. da Silva and co-workers [ 98 ] utilized this method using the antibody-functionalized AuNP bioconjugate. The specific binding of the S antigen to the antibody resulted in an increase of bioconjugate size, with a LOD 5.29 × 10 3 TCID 50 mL −1 (tissue culture infectious dose).…”

Section: Other Sensing Methodsmentioning

confidence: 99%

AuNP-based biosensors for the diagnosis of pathogenic human coronaviruses: COVID-19 pandemic developments

2022

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The outbreak rate of human coronaviruses (CoVs) especially highly pathogenic CoVs is increasing alarmingly. Early detection of these viruses allows treatment interventions to be provided more quickly to people at higher risk, as well as helping to identify asymptomatic carriers and isolate them as quickly as possible, thus preventing the disease transmission chain. The current diagnostic methods such as RT-PCR are not ideal due to high cost, low accuracy, low speed, and probability of false results. Therefore, a reliable and accurate method for the detection of CoVs in biofluids can become a front-line tool in order to deal with the spread of these deadly viruses. Currently, the nanomaterial-based sensing devices for detection of human coronaviruses from laboratory diagnosis to point-of-care (PoC) diagnosis are progressing rapidly. Gold nanoparticles (AuNPs) have revolutionized the field of biosensors because of the outstanding optical and electrochemical properties. In this review paper, a detailed overview of AuNP-based biosensing strategies with the varied transducers (electrochemical, optical, etc.) and also different biomarkers (protein antigens and nucleic acids) was presented for the detection of human coronaviruses including SARS-CoV-2, SARS-CoV-1, and MERS-CoV and lowly pathogenic CoVs. The present review highlights the newest trends in the SARS-CoV-2 nanobiosensors from the beginning of the COVID-19 epidemic until 2022. We hope that the presented examples in this review paper convince readers that AuNPs are a suitable platform for the designing of biosensors. Graphical abstract

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“…Many of the optical immunosensors included in Table 1 are label-free, and a brief presentation of their basic principle/reagents has been considered of interest. Thus, a label-free sensor was reported which employed dynamic light scattering to detect the binding of anti-SARS-CoV-2 spike glycoprotein antibodies conjugated with gold nanoparticles to spike glycoprotein through the increase in the conjugates’ size [ 66 ]. Moreover, a photoluminescence label-free immunosensor for aflatoxin B1 was developed and integrated with a microfluidic cell.…”

Section: Newest Developments In Optical Immunosensors: Bioanalytical ...mentioning

confidence: 99%

“…Bioanalytical applications of optical immunosensors reported in the last couple of years (2021–2022) cover a wide range of areas, from disease diagnosis to food analysis ( Table 1 ). Thus, several of the relevant articles present new optical immunosensors for the detection/quantification of proteins of the SARS-CoV-2 virus or human antibodies developed against them [ 65 , 66 , 71 , 84 ]. Moreover, due to the COVID-19 pandemic outbreak and the consequent high interest in new tools for COVID-19 diagnosis, several recent review articles have presented, among other analytical approaches, research efforts toward development of optical immunosensors associated with COVID-19 [ 43 , 44 , 45 , 46 , 83 ].…”

Section: Newest Developments In Optical Immunosensors: Bioanalytical ...mentioning

confidence: 99%

Recent Developments in the Field of Optical Immunosensors Focusing on a Label-Free, White Light Reflectance Spectroscopy-Based Immunosensing Platform

Karachaliou

Koukouvinos

Goustouridis

et al. 2022

Sensors

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Optical immunosensors represent a research field of continuously increasing interest due to their unique features, which can mainly be attributed to the high-affinity and specific antibodies they use as biorecognition elements, combined with the advantageous characteristics of the optical transducing systems these sensors employ. The present work describes new developments in the field, focusing on recent bioanalytical applications (2021–2022) of labeled and label-free optical immunosensors. Special attention is paid to a specific immunosensing platform based on White Light Reflectance Spectroscopy, in which our labs have gained specific expertise; this platform is presented in detail so as to include developments, improvements, and bioanalytical applications since the mid-2000s. Perspectives on the field are been briefly discussed as well, highlighting the potential of optical immunosensors to eventually reach the state of a reliable, highly versatile, and widely applicable analytical tool suitable for use at the Point-of-Care.

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Detection of SARS-CoV-2 virus via dynamic light scattering using antibody-gold nanoparticle bioconjugates against viral spike protein

Cited by 16 publications

References 41 publications

Controlled release of nitric oxide for enhanced tumor drug delivery and reduction of thrombosis risk

Controlled release of nitric oxide for enhanced tumor drug delivery and reduction of thrombosis risk

AuNP-based biosensors for the diagnosis of pathogenic human coronaviruses: COVID-19 pandemic developments

Recent Developments in the Field of Optical Immunosensors Focusing on a Label-Free, White Light Reflectance Spectroscopy-Based Immunosensing Platform

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