Detection of Sarcocystis spp. in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) in Iran by PCR–RFLP

Hamidinejat, Hossein; Jalali, Mohammad Hossein Razi; Gharibi, Darioush; Molayan, Pedram Haddad

doi:10.1007/s12639-014-0426-6

Cited by 12 publications

(10 citation statements)

References 20 publications

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“…On the other hand, other studies revealed high incidence of macroscopic Sarcocystis in water buffaloes, probably because this was carried out on slaughtered fresh meat, as 67.6 % in Egypt (Ashmawy et al 2014) and 88% in Vietnam (Huong et al 1995),. There were no macrocysts detected in cattle beef in our study and this agrees with Hamidinejat et al (2015) who found no macrocyst in cattle.…”

Section: Discussionsupporting

confidence: 92%

Molecular identification of Sarcocystis species in imported frozen beef in Egypt

De¹,

Ss²,

Ms³

et al. 2017

AJVS

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Key words:Sarcocystis, imported frozen beef, PCR, Histopathology, SequencingIn the present study, we set out to implement and compare the different detection methods that include the peptide digestion, histopathological examination and PCR, to identify Sarcocystis species that can be found in the imported frozen beef. Comparing results from the three identification methods, along with their compatibility and accuracy in diagnosing Sarcocystis spp. are also presented. Finally, a special focus is given to the use of PCR followed by sequencing and the bioinformatics analysis in detecting and comparing Sarcocystis found in the imported frozen beef. A total number of 384 samples of imported frozen beef (280 Indian buffalo and 104 Brazilian cattle meat) were collected from different retail meat shops in Alexandria province, Egypt. Visual meat inspection revealed a low prevalence of macroscopic Sarcocystis spp. in buffalo beef (3.9%) and no macrocysts were detected in cattle beef. Fifty samples were taken from buffalo beef and subjected to peptic digestion, histopathology and PCR resulting in 24%, 38% and 34% positive samples respectively. While the same number of samples were taken from cattle beef resulting in lower infection rates (6%, 2% and 2%). There were no significance difference found between the infection rate according to diagnostic method or type of meat cut. For molecular characterization newly designed primer sets were used to specifically amplify the 18S DNA sequences of the four Sarcocystis spp. in both buffalo and cattle (S.fusiformis, S.buffalonis, S.hirsuta and S.hominis) using the primer BLAST tool on the NCBI of the Genbank. Aligning the 18S rDNA sequences of three positive PCR products revealed high identity percents with three Sarcocystis spp.; S. fusiformis, S. buffalonis and S. hominis. (85%, 92% and 100% respectively).

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Section: Discussionsupporting

confidence: 92%

Molecular identification of Sarcocystis species in imported frozen beef in Egypt

De¹,

Ss²,

Ms³

et al. 2017

AJVS

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“…When raw or undercooked infected beef is consumed by man, it may result in intestinal sarcocystosis (Ifeoma et al, 2013). Sarcocystosis infections in human and animals are caused by singlecelled, cyst-forming, intracellular protozoan parasites in the family of Sarcocystidae (Hamidinejat et al, 2015). Among different known species of Sarcocystis in cattle, only Sarcocystis hominis is important from the public health viewpoint, because of its zoonotic characteristics (Hajimohammadi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Parasitic affections of edible offales of slaughtered animals at El-Shohada abattoir, Monofia governorate, Egypt

El-Meleh

Elmeghnawy

Sabike

et al. 2019

Benha Veterinary Medical Journal

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evaluate the incidence of some parasitic infections (Hydatidosis, Fascioliasis, Cysticercosis and Sarcocystosis) according to seasonal variation. Otherwise, the study aimed to detect the microscopic form of sarcosystic affection in 18 esophageal samples from 6 buffalo (3 suspected and 3 apparently healthy), 3 cattle, 3 sheep, 3 goats and 3 camel by using Polymerase Chain Reaction (PCR). The incidence of hydatidosis was 18%, 0.3% and 0.5% in camel, cattle and buffalo, respectively while it couldn't be detected in young cattle, young buffalo, sheep and goat. The highest total incidence of hydatidosis was recorded in winter (13%). The incidence of fascioliasis was 0.9% in camel, 0.8% in buffalo, 1.2% in cattle and 0.1% in young cattle, but it couldn't be detected in young buffalo, sheep and goat. The cysticercosis couldn't be detected in edible offals of slaughtered animals during the period of the study. Sarcocyst fusiforms was detected in edible offals (22.9% in esophagus and 3.7% in tongue) of 62.8% of buffaloes and 0.2% of the young ones with the highest incidence (42%) was recorded in autumn. PCR reading showed that all 3 samples confirmed positive for suspected buffaloes, one of 3 samples confirmed positive for apparently healthy buffaloes, one of three samples confirmed positive for cattle, 2 of 3 samples confirmed positive for sheep, one of 3 samples confirmed positive for goat and three samples were confirmed negative for camel. The present study concluded that there is a need to educate consumers, food handlers and all others who have access to food about the importance of hygiene and it is necessary for cooking food properly.

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“…(Fischer and Odening 1998;Yang and Zuo 2000). In this study, we amplify partial sequence of 18S rRNA gene from DNA extracted from tissue samples and obtained a product of approximately 900 bp in length by using genus-specific primer (18S9L and 18S1H) similarly as in previous studies (Li et al 2002;Hamidinejat et al 2015). The result of alignment of DNA sequences of this product revealed infection of the frozen buffalo meat with S.cruzi with accession No.HM447193.1 and HM447192.1.…”

Section: Discussionmentioning

confidence: 83%

“…But, molecular methods have been found useful and sensitive in detecting Sarcocystis spp. (Jehle et al 2009;Stojecki et al 2012;Hamidinejat et al 2015). Additionally, molecular techniques can be utilized to distinguish the morphologically comparable sarcocystis cyst in intermediate hosts if it the same or belong to different species (Dahlgren and Gjerde 2007).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Sarcocystis Species Based on Traditional and Molecular Methods in Imported Frozen Buffalo Meat in Egypt

Mousa¹,

Hiekal²,

El-Hoshy³

et al. 2016

AJVS

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Sarcocystis is an intracellular, cyst forming and worldwide distributed parasites. In the present study, fifty five different cuts of imported frozen buffalo meat were collected from different localities of Alexandria markets and were subjected to macroscopic and microscopic examination followed by molecular methods. Polymerase chain reaction used to amplify 18S rRNA gene from the collected samples followed by sequence analysis to detect infections with different Sarcocystis spp. The result revealed that the infection rate in examined sample was 70.9% by molecular methods; while it was 23% and 20% by macroscopic and microscopic examination respectively. Also the percentage of infection was varied among different examined cuts. Comparison of the obtained sequences with published data at the Genebank showed that frozen buffalo meat has multiple sarcocystis infections with S. fusiformis and S.cruzi. This study supported previous findings indicated that water buffalo could be infected with S.cruzi which produced microscopic cyst that has hygienic importance for both veterinary and medical aspects. SO, we recommended to used molecular method for detection sarcocystis infection especially in imported frozen meat to avoid human infection of such zoonotic parasite.

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Detection of Sarcocystis spp. in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) in Iran by PCR–RFLP

Abstract: Sarcocystis species are cyst-forming intracellular protozoan parasites.

Cited by 12 publications

References 20 publications

Molecular identification of Sarcocystis species in imported frozen beef in Egypt

Molecular identification of Sarcocystis species in imported frozen beef in Egypt

Parasitic affections of edible offales of slaughtered animals at El-Shohada abattoir, Monofia governorate, Egypt

Characterization of Sarcocystis Species Based on Traditional and Molecular Methods in Imported Frozen Buffalo Meat in Egypt

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