Detection of Salmonella typhi utilizing bioconjugated fluorescent polymeric nanoparticles

Jain, Swati; Chattopadhyay, S.; Jackeray, Richa; Abid, Zainul; Singh, Harpal

doi:10.1007/s11051-016-3414-1

Cited by 11 publications

(7 citation statements)

References 31 publications

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“…23 Moreover, the introduction of biomolecules (such as antibodies, aptamers, adhesion molecules, and complementary DNA sequences) enables the uorescent sensor to specically target and recognize a single foodborne pathogen, largely improving the accuracy and sensitivity for foodborne pathogen detection. [24][25][26][27] Antibodies are glycoproteins from the native immune response against foreign pathogens, and show many advantages, such as high specicity, easy synthesis, and low cost, which has led to them being employed to construct various immunosensors. 28,29 For example, Ríos-Corripio et al prepared a uorescent bioconjugate based on gold nanoparticles functionalized with uorescein isothiocyanate-labeled antibodies to detect Salmonella.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence immunoassay for the targeted determination of traceListeria monocytogenesbased on immunomagnetic separation and CdZnTe quantum dot indication

Liang

et al. 2022

Anal. Methods

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Section: Introductionmentioning

confidence: 99%

Fluorescence immunoassay for the targeted determination of traceListeria monocytogenesbased on immunomagnetic separation and CdZnTe quantum dot indication

Liang

et al. 2022

Anal. Methods

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“…The enzyme linked immune sorbent assay (ELISA) method is commonly used for detecting pathogens in imported animal-derived feeds, but this method can produce incorrect results due to its cumbersome operational steps. A novel method involving polymer fluorescent nanoparticles as biosensor probes has been described for the detection of Salmonella (Jain et al 2016), which takes only 3 h, but cannot be applied to large volume detection due to its prohibitively high cost.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a duplex real-time qPCR method for detection of Salmonella spp. and Serratia fonticola in fishmeal

Ruan

Wang

Ti-yin³

et al. 2020

AMB Expr

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Salmonella spp. is a high-risk bacterial pathogen that is monitored in imported animal-derived feedstuffs. Serratia fonticola is the bacterial species most frequently confused with Salmonella spp. in traditional identification methods based on biochemical characteristics, which are time-consuming and labor-intensive, and thus unsuitable for daily inspection and quarantine work. In this study, we established a duplex real-time qPCR method with invA- and gyrB-specific primers and probes corresponding to Salmonella spp. and S. fonticola. The method could simultaneously detect both pathogens in imported feedstuffs, with a minimum limit of detection for Salmonella spp. and S. fonticola of 197 copies/μL and 145 copies/μL, respectively (correlation coefficient R2 = 0.999 in both cases). The amplification efficiency for Salmonella spp. and S. fonticola was 98.346% and 96.49%, respectively. Detection of fishmeal was consistent with method GB/T 13091-2018, and all seven artificially contaminated imported feed samples were positively identified. Thus, the developed duplex real-time qPCR assay displays high specificity and sensitivity, and can be used for the rapid and accurate detection of genomic DNA from Salmonella spp. and S. fonticola within hours. This represents a significant improvement in the efficiency of detection of both pathogens in imported feedstuffs.

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“…The enzyme linked immune sorbent assay (ELISA) method is commonly used for detecting pathogens in imported animal-derived feeds, but this method can produce incorrect results due to its cumbersome operational steps. A novel method involving polymer uorescent nanoparticles as biosensor probes has been described for the detection of Salmonella (Jain et al 2016), which takes only 3 h, but cannot be applied to large volume detection due to its prohibitively high cost.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Duplex Real-time qPCR Method for Detection of Salmonella Spp. And Serratia Fonticola in Fishmealestablishment of a Duplex Real-time qPCR Method for Detection of Salmonella Spp. And Serratia Fonticola in Fishmeal

Ruan¹,

Wang²,

Zhang³

et al. 2020

Preprint

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Salmonella spp. is a high-risk bacterial pathogen that is monitored in imported animal-derived feedstuffs. Serratiafonticola is the bacterial species most frequently confused with Salmonella spp. in traditional identification methods based on biochemical characteristics, which are time-consuming and labor-intensive, and thus unsuitable for daily inspection and quarantine work. In this study, we established a duplex real-time qPCR method with invA- and gyrB-specific primers and probes corresponding to Salmonella spp. and S. fonticola. The method could simultaneously detect both pathogens in imported feedstuffs, with a minimum limit of detection for Salmonella spp. and S. fonticola of 197 copies/SL and 145 copies/SL, respectively (correlation coefficient R2 = 0.999 in both cases). The amplification efficiency for Salmonella spp. and S. fonticola was 98.346% and 96.49%, respectively. Detection of clinical samples was consistent with method GB/T 13091-2018, and all seven artificially contaminated imported feed samples were positively identified. Thus, the developed duplex real-time qPCR assay displays high specificity and sensitivity, and can be used for the rapid and accurate detection of genomic DNA from Salmonella spp. and S. fonticola within hours. This represents a significant improvement in the efficiency of detection of both pathogens in imported feedstuffs.

show abstract

Detection of Salmonella typhi utilizing bioconjugated fluorescent polymeric nanoparticles

Cited by 11 publications

References 31 publications

Fluorescence immunoassay for the targeted determination of traceListeria monocytogenesbased on immunomagnetic separation and CdZnTe quantum dot indication

Fluorescence immunoassay for the targeted determination of traceListeria monocytogenesbased on immunomagnetic separation and CdZnTe quantum dot indication

Establishment of a duplex real-time qPCR method for detection of Salmonella spp. and Serratia fonticola in fishmeal

Establishment of a Duplex Real-time qPCR Method for Detection of Salmonella Spp. And Serratia Fonticola in Fishmealestablishment of a Duplex Real-time qPCR Method for Detection of Salmonella Spp. And Serratia Fonticola in Fishmeal

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