Detection of Salmonella spp. from chevon, mutton and its environment in retail meat shops in Anand city (Gujarat), India

Makwana, PP; Nayak, J. B.; Brahmbhatt, M. N.; Chaudhary, Jyoti

doi:10.14202/vetworld.2015.388-392

Cited by 13 publications

(14 citation statements)

References 15 publications

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“…Some studies reported the detection of this gene in all tested Salmonella spp. isolates [49][50][51][52][53][54][55][56][57][58]. On the other side, Moussa et al [59] and Maysa and Abd-Elall [60] couldn't find invA gene in all isolates, same as our study.…”

Section: Resultssupporting

confidence: 83%

Genetic diversity of food originated Salmonella isolates

Karatuğ

Yüksel

Akçelik

et al. 2018

Biotechnology & Biotechnological Equipment

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Currently, Salmonella enterica is the most common bacterial foodborne pathogen, causing serious extraintestinal disease. Typing methods play an important role on pathogens' source tracking, knowing the source(s) of bacteria in pharmaceutical sciences, preventing and controlling the diarrhea and food-poisoning outbreaks. The purpose of this study is to use different moleculer typing methods to determine the genetic variability of 38 foodborne Salmonella isolates that were previously identified by biochemical tests. The methods were evaluated by four molecular techniques including 16S rRNA sequencing, PFGE, PCR-RFLP and invA-spvC PCR. 16S rRNA sequencing results showed that four of the 38 isolates were Escherichia coli, Proteus mirabilis and Citrobacter murliniae, and the others were Salmonella enterica. Thirty-eight strains were subtyped by XbaI-PFGE into 20 profiles with different clusters, while they were subtyped by 16S rRNA-RFLP into 9 profiles with a single cluster. Out of two Salmonella isolates, the invasion gene (invA) was detected in all other Salmonella isolates (94%) and the virulence gene (spvC) was detected in 11% of Salmonella isolates. Our results suggested that the PFGE subtyping is the prominent method for the evaluation and benchmarking of molecular subtyping.

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Section: Resultssupporting

confidence: 83%

Genetic diversity of food originated Salmonella isolates

Karatuğ

Yüksel

Akçelik

et al. 2018

Biotechnology & Biotechnological Equipment

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“…in goat carcasses slaughtered at retail shops of Parbhani city, Maharashtra where zero prevalence of Salmonella in goat samples was recorded [ 13 ]. A study from Anand, Gujarat recorded the incidence of Salmonella in chevon as (3.57%) [ 14 ]. Results of present finding also goes in parallel with the finding from Mumbai wherein workers reported the absence of Salmonella in mutton samples [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation, antibiogram and pathogenicity of Salmonella spp. Recovered from slaughtered food animals in Nagpur region of Central India

et al. 2016

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Aim:To determine the prevalence, antibiogram and pathogenicity of Salmonella spp. in the common food animals slaughtered for consumption purpose at government approved slaughter houses located in and around Nagpur region during a period of 2010-2012.Materials and Methods:A total of 400 samples comprising 50 each of blood and meat from each slaughtered male cattle, buffaloes, pigs and goats were collected. Isolation was done by pre-enrichment in buffered peptone water and enrichment in Rappaport-Vassiliadis broth with subsequent selective plating onto xylose lysine deoxycholate agar. Presumptive Salmonella colonies were biochemically confirmed and analyzed for pathogenicity by hemolysin production and Congo red dye binding assay (CRDA). An antibiotic sensitivity test was performed to assess the antibiotic resistance pattern of the isolates.Results:A total of 10 isolates of Salmonella spp. from meat (3 from cattle, 1 from buffaloes and 6 from pigs) with an overall prevalence of 5% among food animals was recorded. No isolation was reported from any blood samples. Pathogenicity assays revealed 100% and 80% positivity for CRDA and hemolytic activity, respectively. Antimicrobial sensitivity test showed multi-drug resistance. The overall resistance of 50% was noted for trimethoprim followed by ampicillin (20%). A maximum sensitivity (80%) was reported to gentamycin followed by 40% each to ampicillin and trimethoprim, 30% to amikacin and 10% to kanamycin.Conclusion:The presence of multidrug resistant and potentially pathogenic Salmonella spp. in slaughtered food animals in Nagpur region can be a matter of concern for public health.

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“…Salmonella species are Gram-negative facultative anaerobe bacteria and have been isolated from humans, animals, and the environment [8][9][10][11][12][13][14]. Salmonellae are among the major pathogenic bacteria in humans as well as in animals [15,16].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and antibiotic susceptibility of Salmonella spp. from water sources in Tamale, Ghana

2016

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This study investigated the prevalence and antibiotic resistance of Salmonella species isolated from drinking water sources in Tamale Metropolis. Materials and Methods: Isolation of Salmonella species from 275 different drinking water samples (25 each from dam, well, rain, and bottle, 35 from tap, 40 from water trough, and 100 from sachet) was done using a slightly modified method of the Bacteriological Analytical Manual of the Food and Drugs Administration, USA. 34 Salmonella species isolated from the water samples were examined for their susceptibility to nine different antibiotics using the disc diffusion method. The study was carried out from July 2014 to January 2015. Results: The overall prevalence of Salmonella species was 4.36% (12/275). Dam 16.00% (4/25) and well 16.00% (4/25) water samples were the most contaminated source, followed by rain water (stored) 12.00% (3/25) and tap water samples 2.86% (1/35). There were no significant differences among water samples which were positive for Salmonella species (p>0.05); however, dam and well samples that were positive for Salmonella species differ significantly (p<0.05) from bottle water, sachet water, and water trough samples, which were negative for Salmonella species. The 34 Salmonella isolates were highly resistant to erythromycin (E) (100%) and vancomycin (VA) (94.12%). Few isolates exhibited intermediate resistances to ceftriaxone (CRO) (17.65%), gentamicin (CN) (17.65%), tetracycline (14.71%), chloramphenicol (C) (5.88%), ciprofloxacin (CIP) (2.94%), and amoxicillin (AMC) (2.94%). Salmonella isolates also exhibited six different antibiotic resistant patterns (VA-E, VA-E-AMC, VA-E-CRO, VA-E-C, VA-E-CRO-AMC, and VA-E-AMC-CN). The resistant pattern VA-E (with multiple antibiotic resistance index of 0.22) was the commonest. Conclusion:This study indicated that some drinking water sources for humans and animals in Tamale Metropolis are contaminated with Salmonella species which exhibited varying resistance to various antibiotics. Therefore, consumers of water at the Tamale Metropolis are at risk of Salmonella infection from drinking water from positive water sources in the Tamale Metropolis.

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Detection of Salmonella spp. from chevon, mutton and its environment in retail meat shops in Anand city (Gujarat), India

Cited by 13 publications

References 15 publications

Genetic diversity of food originated Salmonella isolates

Genetic diversity of food originated Salmonella isolates

Isolation, antibiogram and pathogenicity of Salmonella spp. Recovered from slaughtered food animals in Nagpur region of Central India

Prevalence and antibiotic susceptibility of Salmonella spp. from water sources in Tamale, Ghana

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