Detection of Salmonella in Foods Using a Reference PN-ISO Method and an Alternative Method Based on Loop-mediated Isothermal Amplification Coupled with Bioluminescence

Evaluation of a commercial loop-mediated isothermal amplification assay, 3MTM Molecular Detection Assay 2 – Campylobacter, for the detection of Campylobacter from poultry matrices

“…LAMP has been coupled to a bioluminescent assay for the detection of amplified products, enabling simultaneous detection and amplification, which provides real-time results in a short run time (Gandelman et al 2010). The LAMP-bioluminescent method offers a simpler, faster and streamlined approach to pathogen detection (Lim et al 2015;Sarowska et al 2016;Yang et al 2016;Hu et al 2017Hu et al , 2018.…”

Rapid Screening for Salmonella in Raw Pet Food by Loop-Mediated Isothermal Amplification

“…Using natural samples, multiple studies have shown that LAMP was either 100% agreeable with culture or PCR and real-time PCR or had higher positive detection rates than those methods (12,19,25,35). For example, one study (35) tested 399 samples in eight food categories, including meat, fish, and vegetables, and both the LAMP-based 3M molecular detection assay (MDA) Salmonella (3M Food Safety, St. Paul, MN) and a reference culture method (PN-ISO 6579:2003) confirmed the presence of Salmonella in 8 samples. In the present study, when screening the 6 raw pet food samples for the absence or presence of Salmonella LAMP T max values by different preenrichment broths and isothermal master mixes when screening for Salmonella in five spiked raw pet food matrices and overall average.…”

Screening of specific nucleic acid targets for Cronobacter sakazakii and visual detection by loop-mediated isothermal amplification and lateral flow dipstick method in powdered infant formula

“…In the detection of foodborne pathogens, molecular biological detection technology as a novel identification method has been widely used, which mainly comprises nucleic acid probe techniques, PCR, real-time PCR, and loop-mediated isothermal amplification (LAMP; Wang et al, 2015;Zhang et al, 2015;Tomas et al, 2018;Liang et al, 2019). Among these, the LAMP platform is relatively simple, rapid, and sensitive, which demonstrates greater promise for practical applications in the field (Dhama et al, 2014;Wu et al, 2014;Sarowska et al, 2016;Fu et al, 2020a). Notably, LAMP combined with a lateral flow dipstick (LFD), which also provides a visual detection technology, has gradually become one of the most effective methods for rapid detection of foodborne pathogens (Yang et al, 2016;Yin et al, 2016;Foo et al, 2017;Jiang et al, 2020).…”