Detection of Salmonella in Foods Using Tecra Salmonella VIA and Tecra Salmonella UNIQUE Rapid Immunoassays and a Cultural Procedure

Paula, Ana; Gelli, Dilma Scala; Landgraf, Mariza; Destro, Maria Teresa; Franco, Bernadette Dora Gombossy de Melo

doi:10.4315/0362-028x-65.3.552

Cited by 13 publications

(6 citation statements)

References 3 publications

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“…Several immunoassay systems are also commercially available for food products (De Paula et al, 2002;Gonzalez et al, 2009;Uyttendaele et al, 2003). The RapidChek Salmonella immunoassay (Strategic Diagnostics Inc., SDIX) uses a lateral flow format for the detection of Salmonella through an antibody which is specific for a target pathogen protein.…”

Section: Introductionmentioning

confidence: 99%

Detection of Salmonella enterica serovar Enteritidis using real time PCR, immunocapture assay, PNA FISH and standard culture methods in different types of food samples

Almeida

Cerqueira

Azevedo

et al. 2013

International Journal of Food Microbiology

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Several methods for the rapid and specific detection of Salmonella in food samples have been described. Here, we compare 4 of those methods in terms of assay time, procedure complexity, detection limit, sensitivity, specificity and accuracy. Milk, eggs and mayonnaise samples were artificially contaminated with Salmonella enterica serovar Enteritidis cell concentrations ranging from 1×10(-2) to 1×10(2) CFU per 25 g or ml of food. Samples were then pre-enriched and analyzed by either: i) real-time PCR, using the iQ-Check Salmonella kit; ii) immunocapture, using the RapidChek SELECT Salmonella; iii) a peptide nucleic acid fluorescence in situ hybridization (PNA FISH) method and iv) the traditional bacteriological method ISO 6579:2002. All methods were able to detect Salmonella in the different types of food matrixes and presented a similar detection level of 1CFU per 25 g or ml of food sample. The immunocapture and the PNA FISH methods proved to be very reliable, as their results were 100% in agreement with the ISO method. However, real-time PCR presented a significant number of false positives, which resulted in a specificity of 55.6% (CI 95%, 31.3-77.6) and an accuracy of 82.2% (CI 95%, 63.2-91.4) for this method. Sensitivity was 100% since no false negative results were observed. In conclusion, the implementation of these molecular techniques, mainly the immunocapture and PNA-FISH methods, provides a reliable and less time-consuming alternative for the detection of Salmonella spp. in food samples.

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Section: Introductionmentioning

confidence: 99%

Detection of Salmonella enterica serovar Enteritidis using real time PCR, immunocapture assay, PNA FISH and standard culture methods in different types of food samples

Almeida

Cerqueira

Azevedo

et al. 2013

International Journal of Food Microbiology

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show abstract

Section: Introductionmentioning

confidence: 99%

“…Immunological methods depend on binding of a specific antibody to an antigen on the bacterium. Several immunoassay systems are commercially available for the detection of Salmonella in food products (dePaula et al 2002). Some of these immunoassays, which also require pre-enrichment of samples, have been validated as rapid detection methods (Andrews et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Adding a selective enrichment step to the iQ-CheckTM real-time PCR improves the detection of Salmonella in naturally contaminated retail turkey meat products

Fakhr¹,

McEvoy²,

Sherwood³

2006

Lett Appl Microbiol

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Aims: The aim of this study was to compare the real‐time iQ‐CheckTMSalmonella kit (Bio‐Rad) with the immunocapture assay RapidCheck®Salmonella method, and a conventional culture method (FSIS, USDA) in detecting Salmonella in naturally contaminated turkey meat products. This study was also designed to determine if a selective enrichment step might improve the real‐time detection of Salmonella. Methods and Results: Using the culture method, Salmonella was recovered from 49 out of 99 retail turkey meat samples collected. RapidCheck® failed to detect 11 Salmonella samples that were positive by the culture method. The iQ‐CheckTM real‐time PCR also failed to detect three samples that were positive by the culture method. However, when carried out after a selective enrichment step, the iQ‐CheckTM real‐time PCR detected all 49 Salmonella samples recovered by the culture method. The iQ‐CheckTM real‐time PCR detected the presence of Salmonella in some samples that were not recovered by the culture method. Conclusions: Adding a selective enrichment step to the iQ‐CheckTM real‐time PCR improves the detection of Salmonella in naturally contaminated turkey meat samples. Significance and Impact of the Study: The iQ‐CheckTMSalmonella real‐time PCR can be used as a rapid method to monitor Salmonella in turkey meat, together with conventional culture methodology.

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“…If serial dilutions of the suspect antigen (e.g., food sample) are made, one may glean relative quantitative, high-throughput data if a spectrophotometer with microtiter plate capabilities is employed for data collection. ELISA has been successfully used to detect either whole-cell antigen targets or products (e.g., virulence determinants) of pathogens such as Salmonella spp., E. coli O157:H7, Campylobacter spp., B. cereus, and L. monocytogenes, among others (Peplow et al 1999;Chen et al 2001;Valdivieso-Garcia et al 2001;Daly et al 2002;de Paula et al 2002;Yeh et al 2002;Bolton et al 2002), in a more user-friendly format than reverse passive latex agglutination assays (explained below). ELISA is automatable, however, and is convenient for large sample numbers with relative ease.…”

Section: Immunoassaysmentioning

confidence: 99%

A review of conventional detection and enumeration methods for pathogenic bacteria in food

Gracias¹,

McKillip²

2004

Can. J. Microbiol.

332

193

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With continued development of novel molecular-based technologies for rapid, high-throughput detection of foodborne pathogenic bacteria, the future of conventional microbiological methods such as viable cell enumeration, selective isolation of bacteria on commercial media, and immunoassays seems tenuous. In fact, a number of unique approaches and variations on existing techniques are currently on the market or are being implemented that offer ease of use, reliability, and low cost compared with molecular tools. Approaches that enhance recovery of sublethally injured bacteria, differentiation among species using fluorogenics or chromogenics, dry plate culturing, differentiation among bacteria of interest using biochemical profiling, enumeration using impedence technology, techniques to confirm the presence of target pathogens using immunological methods, and bioluminescence applications for hygiene monitoring are summarized here and discussed in relation to their specific advantages or disadvantages when implemented in a food microbiology setting.

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Detection of Salmonella in Foods Using Tecra Salmonella VIA and Tecra Salmonella UNIQUE Rapid Immunoassays and a Cultural Procedure

Cited by 13 publications

References 3 publications

Detection of Salmonella enterica serovar Enteritidis using real time PCR, immunocapture assay, PNA FISH and standard culture methods in different types of food samples

Detection of Salmonella enterica serovar Enteritidis using real time PCR, immunocapture assay, PNA FISH and standard culture methods in different types of food samples

Adding a selective enrichment step to the iQ-CheckTM real-time PCR improves the detection of Salmonella in naturally contaminated retail turkey meat products

A review of conventional detection and enumeration methods for pathogenic bacteria in food

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