Detection of Salmonella Enteritidis (sefA) gene, isolated from internal organs of broiler Chickens

Abstract

Salmonella is one of the genus under the family Enterobacteriaceae and is recorded as an important zoonotic pathogen. Salmonella infection (salmonellosis) is a serious problem for chicken farms in different areas because the broiler represents its important host. Salmonella Enteritidis (S. Enteritidis) strain affected mainly the internal organs of the chicken, resulting in elevation of the morbidity and mortality rates, consequently a high economic loss in broiler production. The aim of this work was the detection of the S. Enteritidis (sefA) gene isolated from internal organs of healthy slaughtered broiler chickens. The total samples, 129, were determined as follows: (Liver, kidneys, small intestine, gizzard, and heart blood) are (20,19, 37,30,23) respectively. The samples were collected under microbiological examination. The incidence of Salmonella Species was tabulated as flow: (20%, 10.52%, 37.83%, 6.66%, and 8.69%) from the liver, kidneys, small intestine, gizzard, and heart blood, respectively. Only eight isolates from 24 isolated strains were undergone serological tests, recording one serotype of S. Enteritidis. The high prevalence occurred in the small intestine and liver of apparently healthy broilers, and the lower prevalence occurred in the heart blood, kidneys, and gizzard of internal organs of broilers. The data resulted from antimicrobial sensitivity test application, high resistance rate to vancomycin (64.6%), gentamicin (64.6%); tetracycline (92.8%); chloramphenicol (85.7%); ciprofloxacin (35.7%); levofloxacin, (64.28%); amoxicillin + clavulanic, (100%); streptomycin, (92.85); trimethoprim + sulfamethoxaole (85.7%). While the sensitivity to florfenicol was 100%. Confirmatory PCR technique for detection of (sefA) gene amplification at (310 bp) in 8 isolated strains.