Detection of Salmonella dublin using the vitellogenin 2 promoter of Daphnia magna

Detection of triclosan using 2‐domain hemoglobin promoter of Daphnia magna

“…With advances in genomics, transgenic organisms carrying reporter genes fused with biomarkers are being utilized to monitor toxic substances through gene expression in natural environments. [ 9 ] Among the various reporter genes, the GFP has been widely utilized to visualize the temporal and spatial gene expression patterns in vivo. [ 29 ] Insertion of the three dmag‐Hb2 promoter candidates was carried out upstream of the GFP gene within the pBABE‐GFP vector (Figure S2).…”

“…D. magna and the HeLa cell culture were performed with some modifications to a previously published protocol. [ 9 ] In the HeLa cells culture, fetal bovine serum (FBS; Gibco) was used instead of newborn calf serum.…”

“…Plasmid DNA was prepared from recombinant Escherichia coli BL21 (DE3) according to a previously published protocol. [ 9 ]…”

“…Proteomic analysis (from protein extraction to identification) was carried out according to a previously published protocol. [9] 2.3 | Transfection…”

“…[8] Daphnia magna has the advantages of easy cultivation, a key role in the ecological food web, a short lifespan and high reproductive capacity, and high sensitivity to chemicals. [9] Therefore, D. magna is being used as a ubiquitous model to understand the response to changes in their environment. [10] Many studies have been conducted on the correlation between toxic substances and D. magna at various levels, from molecules, such as genes and proteins, to the whole body.…”