Detection of salinomycin and lasalocid in chicken liver by icELISA based on functional bispecific single-chain antibody (scDb) and interpretation of molecular recognition mechanism

Chen, Yingxian; Zhao, Kunxia; Huang, Jingjie; Li, Miao; Sun, Xiaojuan; Li, Jiancheng

doi:10.1007/s00216-021-03666-0

Cited by 4 publications

(2 citation statements)

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“…The source of accurate structural data relies heavily on bioinformatic prediction methods. In bioinformatic prediction methods, homology modeling can yield protein structure data with reasonably high confidence levels, molecular docking can research molecular interactions, and the combination of homology modeling and molecular docking can predict the binding mode and affinity between scFvs and antigens (Chen et al., 2021; L. Li et al., 2022; Sompunga et al., 2019).…”

Section: Screening Of Scfv Using Phage Display Technologymentioning

confidence: 99%

“…Based on the in‐depth understanding of antibody gene structure, the development of molecular biology technology, and the cooperation of various disciplines, the application of DNA recombination and protein engineering technology can modify scFv at the genetic level and reassemble it into scFv possessing new biological activity (Suurs et al., 2019). Additionally, with the support of simple and easy genetic manipulation, scFv can be further transformed into multivalent small‐molecule antibodies such as diabodies (dimers) and triabodies (trimers) (Chen et al., 2021). In general, scFv exhibits various advantages, including rapid and inexpensive animal‐free production, high potential for improving binding properties, and easy transformation into multispecific molecules to achieve multiple biological effects.…”

Section: Introductionmentioning

confidence: 99%

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Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

et al. 2022

Comp Rev Food Sci Food Safe

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Immunoassays are reliable, efficient, and accurate methods for the analysis of small‐molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) that may be present in food. However, traditional polyclonal and monoclonal antibodies are limited by animal hosts and hinder further development of immunoassays. With the gradual application of phage display technology as an efficient in vitro selection technology, the single‐chain fragment variable (scFv) now provides an exciting alternative to traditional antibodies. Efficiently constructed scFv source libraries and specifically designed biopanning schemes can now yield scFvs possessing specific recognition capabilities. A rational mutation strategy further enhances the affinity of scFv, and allows it to reach a level that cannot be achieved by immunization. Finally, appropriate prokaryotic expression measures ensure stable and efficient production of scFv. Therefore, when developing excellent scFvs, it is necessary to focus on three key aspects of this process that include screening, mutation, and expression. In this review, we analyze in detail the preparation and affinity improvement process for scFv and provide insights into the research progress and development trend of scFv‐based immunoassay methods for monitoring small‐molecule harmful substances.

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Section: Screening Of Scfv Using Phage Display Technologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%