Detection of roe deer, red deer, and hare meat in raw materials and processed products available in Poland

Rak, Lech; Knapik, Karina; Bania, Jacek; Sujkowski, Jakub; Gadzinowski, A.

doi:10.1007/s00217-014-2196-x

Cited by 11 publications

(4 citation statements)

References 32 publications

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“…Adulteration of meat and meat products was not a significant problem for a long time because the meat was consumed fresh or processed to a small extent, but with the improvement of technology and storage facilities for meat adulteration became a major problem. However, meat adulteration methods are based on the use of low-quality meat instead of high-quality [ 11 ], and the consumers are unable to detect adulteration, especially in highly processed meat products [ 12 ]. Food fraud has a large economic potential, believed to be worth several billion dollars every year.…”

Section: Introductionmentioning

confidence: 99%

Adulteration detection technologies used for halal/kosher food products: an overview

2022

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In the Islamic and Jewish religions, there are various restrictions that should be followed in order for food products to be acceptable. Some food items like pork or dog meat are banned to be consumed by the followers of the mentioned religions. However, illegally, some food producers in various countries use either the meat or the fat of the banned animals during food production without being mentioned in the label on the final products, and this considers as food adulteration. Nowadays, halal or kosher labeled food products have a high economic value, therefore deceiving the consumers by producing adulterated food is an illegal business that could make large gains. On the other hand, there is an insistent need from the consumers for getting reliable products that comply with their conditions. One of the main challenges is that the detection of food adulteration and the presence of any of the banned ingredients is usually unnoticeable and cannot be determined by the naked eye. As a result, scientists strove to develop very sensitive and precise analytical techniques. The most widely utilized techniques for the detection and determination of halal/kosher food adulterations can be listed as High-Pressure Liquid Chromatography (HPLC), Capillary Electrophoresis (CE), Gas Chromatography (GC), Electronic Nose (EN), Polymerase Chain Reaction (PCR), Enzyme-linked Immuno Sorbent Assay (ELISA), Differential Scanning Calorimetry (DSC), Nuclear Magnetic Resonance (NMR), Near-infrared (NIR) Spectroscopy, Laser-induced Breakdown Spectroscopy (LIBS), Fluorescent Light Spectroscopy, Fourier Transform Infrared (FTIR) Spectroscopy and Raman Spectroscopy (RS). All of the above-mentioned techniques were evaluated in terms of their detection capabilities, equipment and analysis costs, accuracy, mobility, and needed sample volume. As a result, the main purposes of the present review are to identify the most often used detection approaches and to get a better knowledge of the existing halal/kosher detection methods from a literature perspective.

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Section: Introductionmentioning

confidence: 99%

Adulteration detection technologies used for halal/kosher food products: an overview

2022

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“…Fraudulent labeling of game meat products can only be detected by applying specific and sensitive analytical methods. Both conventional polymerase chain reaction (PCR) and real-time PCR assays have been developed for the detection of a variety of game meat species in food [6][7][8][9][10]. According to the Codex Alimentarius Austriacus, a collection of haven, Germany; Institute of Hygiene and Environment, Hamburg, Germany; Max Rubner-Institut, Kulmbach, Germany; Official Food Control Authority of the Canton Zurich, Zurich, Switzerland; Saxon State Institute of Health and Veterinary Affairs, Dresden, Germany; Saxony-Anhalt State Office for Consumer Protection, Halle, Germany; State Laboratory Berlin-Brandenburg, Berlin, Germany; State Office Laboratory Hessen, Gießen, Germany; State Office of Agriculture, Food Safety and Fisheries Mecklenburg-Vorpommern, Rostock, Germany.…”

Section: Introductionmentioning

confidence: 99%

Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland

Druml

Uhlig²,

Simon³

et al. 2021

Foods

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Game meat products are particularly prone to be adulterated by replacing game meat with cheaper meat species. Recently, we have presented a real-time polymerase chain reaction (PCR) assay for the identification and quantification of roe deer in food. Quantification of the roe deer content in % (w/w) was achieved relatively by subjecting the DNA isolates to a reference real-time PCR assay in addition to the real-time PCR assay for roe deer. Aiming at harmonizing analytical methods for food authentication across EU Member States, the real-time PCR assay for roe deer has been tested in an interlaboratory ring trial including 14 laboratories from Austria, Germany, and Switzerland. Participating laboratories obtained aliquots of DNA isolates from a meat mixture containing 24.8% (w/w) roe deer in pork, roe deer meat, and 12 meat samples whose roe deer content was not disclosed. Performance characteristics included amplification efficiency, level of detection (LOD95%), repeatability, reproducibility, and accuracy of quantitative results. With a relative reproducibility standard deviation ranging from 13.35 to 25.08% (after outlier removal) and recoveries ranging from 84.4 to 114.3%, the real-time PCR assay was found to be applicable for the detection and quantification of roe deer in raw meat samples to detect food adulteration.

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“…PCR, a representative DNA-based detection method, has been utilized for species identification in various fields [ 15 , 16 , 17 , 18 ]. For deer species, PCR-based detection methods, such as conventional PCR and real-time PCR, have been developed [ 3 , 8 , 19 ]. To differentiate closely related animal species, the development of specific primers for a target species is very crucial.…”

Section: Introductionmentioning

confidence: 99%

Species Identification of Red Deer (Cervus elaphus), Roe Deer (Capreolus capreolus), and Water Deer (Hydropotes inermis) Using Capillary Electrophoresis-Based Multiplex PCR

Kim

Lee

Suh

et al. 2020

Foods

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To provide consumers correct information on meat species, specific and sensitive detection methods are needed. Thus, we developed a capillary electrophoresis-based multiplex PCR assay to simultaneously detect red deer (Cervus elaphus), roe deer (Capreolus capreolus), and water deer (Hydropotes inermis). Specific primer sets for these three species were newly designed. Each primer set only amplified target species without any reactivity against non-target species. To identify multiple targets in a single reaction, multiplex PCR was optimized and combined with capillary electrophoresis to increase resolution and accuracy for the detection of multiple targets. The detection levels of this assay were 0.1 pg for red deer and roe deer and 1 pg for water deer. In addition, its applicability was demonstrated using various concentrations of meat DNA mixtures. Consequently, as low as 0.1% of the target species was detectable using the developed method. This capillary electrophoresis-based multiplex PCR assay for simultaneous detection of three types of deer meat could authenticate deer species labeled on products, thus protecting consumers from meat adulteration.

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Detection of roe deer, red deer, and hare meat in raw materials and processed products available in Poland

Cited by 11 publications

References 32 publications

Adulteration detection technologies used for halal/kosher food products: an overview

Adulteration detection technologies used for halal/kosher food products: an overview

Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland

Species Identification of Red Deer (Cervus elaphus), Roe Deer (Capreolus capreolus), and Water Deer (Hydropotes inermis) Using Capillary Electrophoresis-Based Multiplex PCR

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