Detection of RNA viruses in potato seed-tubers from northern Antioquia (Colombia)

García, Andrea; Giraldo, Susana; Higuita, Mónica; Hoyos, Rodrigo; Marín, Mauricio; Gutiérrez, Pablo

doi:10.1590/0034-737x202370050013

Cited by 1 publication

(1 citation statement)

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“…et al, 2012b) The first report of Tobacco necrosis virus (TNVD) in Colombia occurred recently in 2023, specifically pinpointing TNVD as the identified virus in potato seed-tubers originating from the northern Antioquia region, nestled within the Andean landscape of Colombia. This study utilized NGS RNA-seq technology in Solanum phureja and facilitated the retrieval of the partial sequence of the TNVD genome (García et al, 2023). TNVD is classified within the genus Betanecrovirus (Rochon, 1999; C. M. Varanda et al, 2014b; Verdin et al, 2018a), standing as a sister clade to the Alphanecrovirus.…”

Section: Discussionmentioning

confidence: 99%

Novel PotentialCannabisPathogen: Discovery of Tobacco necrosis virus A in a Diseased ColombianCannabis sativaPlant

Lopez-Jimenez,

Palacio-Torres,

Alzate

2024

Preprint

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Plant viral infections pose a significant threat to global crop productivity. Despite their profound impact on agriculture, plant viruses have been relatively understudied, primarily due to technological limitations associated with classical molecular methods. However, the advent of NGS RNA-seq analysis has revolutionized virus characterization in environmental settings, overcoming previous limitations and providing a powerful tool for studying plant viruses. In an RNA-seq experiment conducted on a diseased Colombian Cannabis sativa hemp plant, we identified a linear single-stranded RNA (ssRNA) genome belonging to Tobacco Necrosis Virus A (TNVA), a common cause of necrotic lesions in plants such as tobacco and tulipa. The affected Cannabis sativa hemp plant exhibited severe symptoms, including alterations in pigmentation, leaf morphology such as chlorosis, necrotic tissue formation, and surface wear on the leaves. The complete genome sequence of the Cannabis sativa TNVA was 3,656 nucleotides long, containing five putative ORFs, and was classified in the family Tombusviridae, genus Alphanecrovirus, and belonging to the Necro-like clade based on RdRp protein phylogenetic analysis. Our analysis revealed a well-conserved RdRp protein among the Alphanecroviruses, with 89% of the amino acid residues in the peptide being entirely conserved. In contrast, the coat protein exhibited significantly higher variability, with only 49.3% of the residues being 100% conserved. Regarding the viral genome expression of Cannabis sativa TNVA, we observed that the virus was highly abundant in the leaves of the diseased plant, ranking among the topmost abundant transcripts, occupying the percentile position of 3.06%. Overall, our study generated the first reference genome of TNVA virus in the tropical region and reported the first case of this virus infecting a Cannabis sativa plant.

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Section: Discussionmentioning

confidence: 99%

Novel PotentialCannabisPathogen: Discovery of Tobacco necrosis virus A in a Diseased ColombianCannabis sativaPlant

Lopez-Jimenez,

Palacio-Torres,

Alzate

2024

Preprint

View full text Add to dashboard Cite

show abstract

Detection of RNA viruses in potato seed-tubers from northern Antioquia (Colombia)

Abstract: This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original work is properly cited.

Cited by 1 publication

References 46 publications

Novel PotentialCannabisPathogen: Discovery of Tobacco necrosis virus A in a Diseased ColombianCannabis sativaPlant

Novel PotentialCannabisPathogen: Discovery of Tobacco necrosis virus A in a Diseased ColombianCannabis sativaPlant

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