Detection of Rickettsia hoogstraalii , Rickettsia helvetica , Rickettsia massiliae , Rickettsia slovaca and Rickettsia aeschlimannii in ticks from Sardinia, Italy

Chisu, Valentina; Leulmi, Hamza; Masala, Giovanna; Piredda, Mariano; Foxi, Cipriano; Parola, Philippe

doi:10.1016/j.ttbdis.2016.12.007

Cited by 62 publications

(42 citation statements)

References 42 publications

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“…marginatum in 1997 from Morocco (Beati, Meskini, Thiers, & Raoult, 1997) and is now reported in the whole Mediterranean area (Parola et al, 2013). (Chisu et al, 2017 andSelmi et al, 2017), mainland France (Beati, Finidori, & Raoult, 1993) and Spain (Fernández-Soto, Pérez-Sánchez, Encinas-Grandes, & Sanz, 2006).…”

Section: Other Tbps Identified In Corsicamentioning

confidence: 85%

“…Rickettsia slovaca was first isolated in 1968 in a D. marginatus specimen collected in Slovakia and is largely spread in the Mediterranean area. It was found in Sardinia and continental Italy (Chisu et al, and Selmi et al, ), mainland France (Beati, Finidori, & Raoult, ) and Spain (Fernández‐Soto, Pérez‐Sánchez, Encinas‐Grandes, & Sanz, ). The first proven human case of R .…”

Section: Discussionmentioning

confidence: 96%

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Tick‐borne pathogens in ticks (Acari: Ixodidae) collected from various domestic and wild hosts in Corsica (France), a Mediterranean island environment

Grech‐Angelini

Stachurski

Vayssier‐Taussat

et al. 2019

Transbound Emerg Dis

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Corsica is a mountainous French island in the north‐west of the Mediterranean Sea presenting a large diversity of natural environments where many interactions between humans, domestic animals and wild fauna occur. Despite this favourable context, tick‐borne pathogens (TBPs) have not systematically been investigated. In this study, a large number of TBPs were screened in ticks collected over a period of one year from domestic and wild hosts in Corsica. More than 1,500 ticks belonging to nine species and five genera (Rhipicephalus, Hyalomma, Dermacentor, Ixodes and Haemaphysalis) were analysed individually or pooled (by species, gender, host and locality). A real‐time microfluidic PCR was used for high‐throughput screening of TBP DNA. This advanced methodology enabled the simultaneous detection of 29 bacterial and 12 parasitic species (including Borrelia, Anaplasma, Ehrlichia, Rickettsia, Bartonella, Candidatus Neoehrlichia, Coxiella, Francisella, Babesia and Theileria). The Crimean–Congo haemorrhagic fever (CCHF) virus was investigated individually in tick species known to be vectors or carriers of this virus. In almost half of the tick pools (48%), DNA from at least one pathogen was detected and eleven species of TBPs from six genera were reported. TBPs were found in ticks from all collected hosts and were present in more than 80% of the investigated area. The detection of DNA of certain species confirmed the previous identification of these pathogens in Corsica, such as Rickettsia aeschlimannii (23% of pools), Rickettsia slovaca (5%), Anaplasma marginale (4%) and Theileria equi (0.4%), but most TBP DNA identified had not previously been reported in Corsican ticks. This included Anaplasma phagocytophilum (16%), Rickettsia helvetica (1%), Borrelia afzelii (0.7%), Borrelia miyamotoi (1%), Bartonella henselae (2%), Babesia bigemina (2%) and Babesia ovis (0.5%). The high tick infection rate and the diversity of TBPs reported in this study highlight the probable role of animals as reservoir hosts of zoonotic pathogens and human exposure to TBPs in Corsica.

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Section: Other Tbps Identified In Corsicamentioning

confidence: 85%

Section: Discussionmentioning

confidence: 96%

Tick‐borne pathogens in ticks (Acari: Ixodidae) collected from various domestic and wild hosts in Corsica (France), a Mediterranean island environment

Grech‐Angelini

Stachurski

Vayssier‐Taussat

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

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“…Israelensis, Rickettsia conorii subsp. Indica, Rickettsia raoulti, Rickettsia helvetica, and Rickettsia hoogstraalii, [1,2,[11][12][13][14].…”

Section: Introductionunclassified

Diversity of Rickettsia in Ticks Collected in Abruzzi and Molise Regions (Central Italy)

et al. 2019

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Rickettsiae have worldwide occurrence and rickettsiosis are widely recognized as emerging infections in several parts of the world. For decades, it was thought that a single pathogenic tick-borne spotted fever group (SFG), Rickettsia, occurred in each continent. Nowadays, thanks to molecular biology, new species of Rickettsia responsible for disease in humans are continuously identified worldwide. In a framework of diagnostic activities of the Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Gaporale" and considering some reports of suspected human clinical cases of rickettsiosis, a survey on ticks collected form animals and humans was carried out with the aim to identify the Rickettsia species circulating in Abruzzi and Molise regions. A total of 603 ticks, previously identified at species level by morphology, pooled into 178 tick samples, were tested by pan-Rickettsia RealTime PCR. DNA from specimens positive for Rickettsia spp. was then sequenced in order to identify the Rickettsia species involved. The highest infection rate was detected in Dermacentor marginatus followed by Ixodes ricinus. The selected targets for this purpose were OmpA and gltA. Rickettsia slovaca, Rickettsia monacensis, Rickettsia massiliae, Rickettsia conorii, Rickettsia aeschlimannii, Rickettsia helvetica, Rickettsia raoultii, and Rickettsia felis -like organisms were identified in this study. These are the first data available in the literature for the circulation of SFG Rickettsia species in the selected geographical area. Results made evidence of high rate of infection in ticks. All Rickettsia species detected have been previously involved in human infection. The diversity of Rickettsia detected, and tick species collected reflects the biodiversity in term of wildlife and environment of the area. An association between Rickettsia species, ticks, and the relationships with vertebrate host species are discussed. Due to the peculiar eco-biology of each Rickettsia species, the use of diagnostic tools able to identify Rickettsia at the species level is thus recommended in order to assess the risk for humans and to elucidate more precise etiological diagnosis in clinical cases.

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“…(8,27). DNA tego patogenu zostało także wykryte u kleszczy Haemaphysalis parva zebranych z dzikich zwierząt w Turcji oraz we Włoszech: u 2/49 kleszczy Haemaphysalis punctata pozyskanych z owiec oraz 3/14 kleszczy H. sulcata pozyskanych z muflonów (28). W Grecji DNA R. hoogstraalii zostało stwierdzone u 5/187 kleszczy pozyskanych z owiec i kóz -H. sulcata i H. parva (29).…”

Section: Rickettsia Monacensisunclassified

Tick-borne rickettsioses in Europe – a review

et al. 2020

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Tick-borne rickettsioses are emerging diseases in Europe. The Mediterranean region is endemic for rickettsioses, but the disease can also occur in other European countries. Ticks can be a vector of these bacteria. Spotted fevers caused by rickettsiae have nonspecific symptoms and are difficult to diagnose. The results of serological tests are positive many days after the onset of symptoms. PCR method can be useful in the diagnostic process. In the article we discuss the problem of rickettsia in Europe.

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Detection of Rickettsia hoogstraalii , Rickettsia helvetica , Rickettsia massiliae , Rickettsia slovaca and Rickettsia aeschlimannii in ticks from Sardinia, Italy

Cited by 62 publications

References 42 publications

Tick‐borne pathogens in ticks (Acari: Ixodidae) collected from various domestic and wild hosts in Corsica (France), a Mediterranean island environment

Tick‐borne pathogens in ticks (Acari: Ixodidae) collected from various domestic and wild hosts in Corsica (France), a Mediterranean island environment

Diversity of Rickettsia in Ticks Collected in Abruzzi and Molise Regions (Central Italy)

Tick-borne rickettsioses in Europe – a review

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