Detection of Rickettsia helvetica and Candidatus R. tarasevichiae DNA in Ixodes persulcatus ticks collected in Northeastern European Russia (Komi Republic)

Kartashov, M. Yu.; Глушкова, Л И; Mikryukova, Tamara P.; Korabel'nikov, I V; Egorova, Yulia I.; Тупота, Н. Л.; Протопопова, Е. В.; Konovalova, Svetlana N.; Va, Ternovoĭ; Loktev, V. B.

doi:10.1016/j.ttbdis.2017.04.001

Cited by 15 publications

(16 citation statements)

References 27 publications

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“…Serological or molecular tools have been used to detect R. helvetica infection in samples from patients with suspected Lyme neuroborreliosis in the Netherlands [10], with unexplained fever following a tick bite in France and Italy [11] and with rash, febrile illness and meningitis in Sweden [8,9]. Rickettsia helvetica, a tick-borne rickettsiae species, is also frequently detected in Europe and Asia [2,28,29], being reported to be the prevalent Rickettsia species in some of the regions, e.g. in Germany [30], Slovakia [31] and Sakhalin Island in Russia [29].…”

Section: Discussionmentioning

confidence: 99%

Rickettsia spp. in rodent-attached ticks in Estonia and first evidence of spotted fever group Rickettsia species Candidatus Rickettsia uralica in Europe.

Vikentjeva

Geller

Remm

et al. 2021

Preprint

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BACKGROUND: Rickettsia spp. are human pathogens that cause a number of diseases and are transmitted by arthropods, such as ixodid ticks. Estonia is one of few regions where the distribution area of two medically important tick species, Ixodes persulcatus and I. ricinus, overlaps. The presence of the nidicolous rodent-associated I. trianguliceps has also recently been shown in Estonia. Although there is no data available in Estonia on human disease caused by tick-borne Rickettsia spp., the presence of three Rickettsia species in non-nidicolous ticks was also previously reported. The aim of this study was to detect, identify and partially characterize Rickettsia species in nidicolous and non-nidicolous ticks attached to rodents.RESULTS: Larvae and nymphs of I. ricinus (n = 1004), I. persulcatus (n = 75) and I. trianguliceps (n = 117) removed from rodents and shrews caught in different parts of Estonia were studied for the presence of Rickettsia spp. by nested PCR. Ticks were collected from 314 small animals of 5 species (Myodes glareolus (bank voles), Apodemus flavicollis (yellow necked mice), A. agrarius (striped field mice), Microtus subterranius (pine voles) and Sorex araneus (common shrews)). Rickettsial DNA was detected in 8.7% (103/1186) of the studied ticks. In addition to R. helvetica previously found in questing ticks, this study reports the first identification of the recently described I. trianguliceps-associated Candidatus R. uralica west of the Ural Mountains.

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Section: Discussionmentioning

confidence: 99%

Rickettsia spp. in rodent-attached ticks in Estonia and first evidence of spotted fever group Rickettsia species Candidatus Rickettsia uralica in Europe.

Vikentjeva

Geller

Remm

et al. 2021

Preprint

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“…Ticks were transported on wet wipes in a cool bag at +4°C and stored at -18…-24°C. For tick species identification, the mitochondrial genome-encoded 16S rRNA fragments were sequenced [12].…”

Section: Methodsmentioning

confidence: 99%

Genetic Variability of Tick-Borne Encephalitis Virus Genome 5'-UTR from Northern Eurasia

et al. 2021

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This paper reports the analysis of the nucleotide sequences of the 5'-untranslated region (5'-UTR) of tick-borne encephalitis virus (TBEV) genomic RNA isolated from 39 individual taiga ticks collected in several regions of Northern Eurasia. The sequences of 5'-UTRs of the Siberian and Far East TBEV genotypes were 89% and 95% identical to the prototype strains (Zausaev and 205), respectively. The detected nucleotide substitutions were typical for these two TBEV genotypes, which made possible unambiguous identification. Both conservative and variable motifs were detected in the 5'-UTR RNA. The B2, C1, and C2 elements of the Y-shaped 5'-UTR structure and the presumable viral RNA-dependent RNA-polymerase binding site were the most variable. The A2, CS A, CS B elements as well as the start codon were conservative. Interestingly, five substitutions in the 5'-UTR C1 variable element of the TBEVs isolated in different geographical regions were strictly conservative, while 11 different substitutions were detected in this element among the laboratory TBEV variants. A little less that a third of all nucleotide substitutions were mapped outside the main elements of the Y-shaped structure. In general, nucleotide substitutions were localized to stem structures, not being found in the hairpin regions of the TBEV 5'-UTR. The results indicated significant variability of the genomic RNA 5'-UTR in the TBEV laboratory strains and field isolates obtained from different geographical regions. It has been suggested that genetic variability of 5'-UTR is characteristic of the TBEV genome 5'-UTR organization and may serve as a structural basis for virus efficient replication in various avian, mammalian, and ixodic tick cells.

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“…Serological or molecular tools have been used to detect R. helvetica infection in samples from patients with suspected Lyme neuroborreliosis in the Netherlands [9], with unexplained fever following a tick bite in France and Italy [10] and with rash, febrile illness and meningitis in Sweden [7,8]. R. helvetica is also the most prevalent tick-borne Rickettsiae species as elsewhere in Europe and Asia [5,34] as well as in the Estonian tick population, comprising over 95% of all Rickettsia species detected in questing [11] as well as in rodent-attached ticks as our current study results show. While there are no clinical reports to date of illness caused by R. helvetica in Estonia, the detection of this tick-borne pathogen at prevalence rates compatible to those for B. burgdorferi s. l. [14], R. helvetica should be considered in the surveillance of tick-borne diseases in Lyme endemic regions.…”

Section: Rickettsia Screening and Rickettsia Speciesmentioning

confidence: 99%

Rickettsia spp. in rodent-attached ticks and first evidence of Spotted fever Group Rickettsia species Candidatus Rickettsia uralica in Europe.

Vikentjeva

Geller

Remm

et al. 2020

Preprint

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BACKGROUND Rickettsia spp. are human pathogens that cause a number of diseases and are transmitted by arthropods, including ixodid ticks. Estonia contributes a region, where the distribution area of two exophilic tick species of known medical importance, Ixodes persulcatus and I. ricinus, overlap. The presence of the nidicolous rodent-associated I. trianguliceps has recently been shown for Estonia. Although there is no Estonian data available on human disease caused by tick-borne Rickettsia spp., the presence of three Rickettsia species in non-nidicolous ticks, albiet at very dissimilar rates, was also previously reported. The aim of this studywas to screen, identify and characterize Rickettsia species in nidicolous and non-nidicolous ticks attached to rodents. RESULTS Nymphs and larvae of I. ricinus ( n = 1004), I . persulcatus ( n = 75) and I. trianguliceps ( n = 117) attached to rodents and shrews caught in different parts of Estonia were studied for the presence of Rickettsia spp. by nested PCR. Ticks were removed from 314 small animals of 5 species (bank voles Myodes glareolus , yellow necked mice Apodemus flavicollis , striped field mice A. agrarius, pine voles M. subterranius and common shrews S. araneus) . Rickettsial DNA was detected in 8,7% (103/1186) studied ticks. In addition to R. helvetica, previously found in questing ticks, this study reports the first identification of the recently described I. trianguliceps- associated Candidatus R. uralica in west of the Ural.

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Detection of Rickettsia helvetica and Candidatus R. tarasevichiae DNA in Ixodes persulcatus ticks collected in Northeastern European Russia (Komi Republic)

Cited by 15 publications

References 27 publications

Rickettsia spp. in rodent-attached ticks in Estonia and first evidence of spotted fever group Rickettsia species Candidatus Rickettsia uralica in Europe.

Rickettsia spp. in rodent-attached ticks in Estonia and first evidence of spotted fever group Rickettsia species Candidatus Rickettsia uralica in Europe.

Genetic Variability of Tick-Borne Encephalitis Virus Genome 5'-UTR from Northern Eurasia

Rickettsia spp. in rodent-attached ticks and first evidence of Spotted fever Group Rickettsia species Candidatus Rickettsia uralica in Europe.

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