Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA

Abstract

Abstract:Bovine Respiratory Disease is one of the most important diseases with serious financial losses for the cattle industry in worldwide. The aim of this study was to detect the associations between respiratory viruses; bovine herpes virus 1, bovine viral diarrhea virus, bovine respiratory syncytial virus, and Para influenza virus 3 statuses of a herd and bovine respiratory disease occurrence. Present study describes virological distribution of bovine respiratory viruses in nonvaccinated cattle (for mentioned infections) of Central Anatolia, Turkey. A total of 24 lung tissue samples were collected during the December 2012 to January 2013 from cattle that died after manifesting clinical signs of respiratory system. Samples were successfully homogenized. Tissue samples were analyzed for detecting antigens by commercially available direct ELISA kit. BRSV antigens were detected in lung tissues 4 out of 24 tested cattle with a percentage of 16.6%, whereas BHV-1, BVDV and PI-3 were not found. BRSV may be common reason of respiratory diseases in herds. It has been also offered advice about prevention of respiratory viral infection for health planning. In conclusion, existence of BRSV infection is still defined and may play an important role in the respiratoric viral infection of cattle.

Identification and molecular characterisation of bovine parainfluenza virus-3 and bovine respiratory syncytial virus - first report from Turkey

“…BPIV3 and BRSV were detected by other researchers with serological and virological methods in cases of lower respiratory tract infections in cattle in Turkey (2, 6, 41). However, these studies were not performed by molecular methods and did not include genetic characterisation as we have done.…”

“…Data on virological detection of these agents in Turkey is limited (2, 6), but there are more studies on seroprevalence of these viruses among cattle herds. The studies reported lowest and highest seropositivity of 11% (1) and 92.8% (13) for BPIV3 and 28% (1) and 94% (13) for BRSV.…”

Comparative detection of bovine herpesvirus-1 using antigen ELISA, immunohistochemistry and immunofluorescence methods in cattle with pneumonia

“…BoHV-1 positivity was found as 2% in 250 camel lung samples in a study via antigen ELISA (43). It has been reported that no positivity was detected in lung samples of 24 cattle in antigen ELISA study performed to determine BoHV-1 in Turkey (44). So, in the light of the examined literature, the present study is the first study to detect BoHV-1 positivity from cattle lung tissues by antigen ELISA method.…”

Detection of Respiratory Viral Antigens in Nasal Swabs of Bovine by Sandwich ELISA

“…However, Masot et al (1993) considered ELISA as a diagnostic tool and most suitable technique for identifying BRSV antigen in the lung tissue. The detection of viral antigen from pneumonic lung tissue also tried by Avci et al (2014) by antigen capture direct ELISA and 16.6% positivity recorded for BRSV antigen whereas could not find presence of BoHV-1, BPI3V and BVDV.…”

“…Bovine Parainfluenza-3 virus (BPI3V) is also associated with respiratory infections, characterized by rhinitis, acute and silent fever nasal secretions (Solis-Calderon et al, 2003) (Fulton and Confer 2012). However ELISA has been test of choice for detection of antigen and antibody due to advantage to perform this test within a short time frame in the laboratory and is more efficient in the differential diagnosis as combined infection can also be detected simultaneously for different associated viral agents (Avci et al, 2014). The present study planned to detect the various respiratory viral antigens in nasal excretions of cattle and buffaloes and determine the BRD occurrence by sandwich ELISA.…”