Detection of resistance to acetohydroxyacid synthase inhibitors in Amaranthus sp. using DNA polymorphisms

Corbett, Cheryl-Ann L.; Tardif, François J.

doi:10.1016/j.pestbp.2008.06.007

Cited by 6 publications

(7 citation statements)

References 18 publications

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“…was monitored by PCR‐RFLP assays with this enzyme,64 and it has been used to identify interspecific hybrids of Palmer amaranth and common waterhemp where the W 574 L allele was used as a selectable marker 65. This enzyme has been used to detect this substitution in resistant redroot and green pigweeds 66…”

Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

“…ALS inhibitor resistance in A. powellii and A. retroflexus conferred by the A 122 T substitution was successfully detected by digesting a 499 bp PCR fragment with Sfa N I. Co‐detection of the A 205 V substitution with the same enzyme was possible in A. retroflexus using the same fragment 66. In each case the mutation resulted in the loss of a recognition site.…”

Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

“…Double digestion of a 1097 bp ALS fragment with Mbo I and Sfa N I allowed detection of the A 122 T, A 205 V and D 376 E substitutions in A. powellii and A. retroflexus , as both enzymes use the same reaction conditions 30. Similarly, digestion of a 659 bp fragment with Bfa I and Mfe I allowed detection of the W 574 L and S 653 T substitutions in A. retroflexus and A. powellii 66…”

Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

“…Five different allele‐specific primers in five separate PASAs were able to differentiate the A 122 T, A 205 V, D 376 E, W 574 L and S 653 T alleles from wild‐type DNA in Amaranthus spp 30, 66. Two non‐specific primers were incorporated in each of the assays (one forward and one reverse) to produce internal control fragments that appeared in all successful PCRs.…”

Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

“…Corbett and Tardif66 were able to consolidate four different PASAs into one ‘tetraplex’ PASA for the detection of four different alleles in the ALS gene (A 122 T, A 205 V, W 574 L and S 653 T) of green and redroot pigweeds. All the allele‐specific primers were specific at the same annealing temperature (46 °C), and each allele‐specific primer produced a PCR amplicon of a specific size that was easily differentiated from the other allele‐specific amplicons by gel electrophoresis.…”

Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

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Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA‐based techniques: a review

Corbett

Tardif

2006

Pest Management Science

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Resistance to herbicides inhibiting acetolactate synthase (ALS) has been increasing at a faster rate than in any other herbicide group. The great majority of these cases are due to various single-nucleotide polymorphisms in the ALS gene endowing target site resistance. Many diagnostic techniques have been devised in order to confirm resistance and help producers to adopt the best management strategies. Recent advances in DNA technologies coupled with the knowledge of sequence information have allowed the development of accurate and rapid diagnostic tests. While whole plant-based diagnostic techniques such as seedling bioassays or enzyme-based in vitro bioassays provide accurate results, they tend to be labour- and/or space-intensive and will only respond to the particular herbicides tested, making resolution of cross-resistance patterns more difficult. Successful DNA-based diagnosis of ALS inhibitor resistance has been achieved with three main techniques, (1) restriction fragment length polymorphism, (2) polymerase chain reaction amplification of specific alleles and (3) denaturing high-performance liquid chromatography. All DNA-based techniques are relatively rapid and provide clear identification of the mutations causing resistance. Resistance based on non-target mechanisms is not identified by these DNA-based methods; however, given the prevalence of target site-based ALS inhibitor resistance, this is a minor inconvenience.

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Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

Section: Dna‐based Tests For Mutation Detectionmentioning

confidence: 99%

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Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA‐based techniques: a review

Corbett

Tardif

2006

Pest Management Science

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Herbicide‐Resistant Weeds in Rice

2023

Weed Science and Weed Management in Rice and Cereal‐Based Cropping Systems

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Resistance to AHAS inhibitor herbicides: current understanding

Powles

2014

Pest Management Science

374

442

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Acetohydroxyacid synthase (AHAS) inhibitor herbicides currently comprise the largest site-of-action group (with 54 active ingredients across five chemical groups) and have been widely used in world agriculture since they were first introduced in 1982. Resistance evolution in weeds to AHAS inhibitors has been rapid and identified in populations of many weed species. Often, evolved resistance is associated with point mutations in the target AHAS gene; however non-target-site enhanced herbicide metabolism occurs as well. Many AHAS gene resistance mutations can occur and be rapidly enriched owing to a high initial resistance gene frequency, simple and dominant genetic inheritance and lack of major fitness cost of the resistance alleles. Major advances in the elucidation of the crystal structure of the AHAS (Arabidopsis thaliana) catalytic subunit in complex with various AHAS inhibitor herbicides have greatly improved current understanding of the detailed molecular interactions between AHAS, cofactors and herbicides. Compared with target-site resistance, non-target-site resistance to AHAS inhibitor herbicides is less studied and hence less understood. In a few well-studied cases, non-target-site resistance is due to enhanced rates of herbicide metabolism (metabolic resistance), mimicking that occurring in tolerant crop species and often involving cytochrome P450 monooxygenases. However, the specific herbicide-metabolising, resistance-endowing genes are yet to be identified in resistant weed species. The current state of mechanistic understanding of AHAS inhibitor herbicide resistance is reviewed, and outstanding research issues are outlined.

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Detection of resistance to acetohydroxyacid synthase inhibitors in Amaranthus sp. using DNA polymorphisms

Cited by 6 publications

References 18 publications

Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA‐based techniques: a review

Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA‐based techniques: a review

Herbicide‐Resistant Weeds in Rice

Resistance to AHAS inhibitor herbicides: current understanding

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