Detection of Renibacterium salmoninarum by a modified peroxidase-antiperoxidase(PAP) procedure.

Sakai, Masahiro; Koyama, Gentarou; Atsuta, Shizuo; Kobayashi, Masanori

doi:10.3147/jsfp.22.1

Cited by 18 publications

(18 citation statements)

References 8 publications

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“…grew more quickly on KDM2, and growth on KDM2 also produced more colonies. This is in contrast to the findings of Austin et al (1983), Sakai et al (1987), and Gudmundsdottir et al (1991), who found more positive flsh on SKDM than on KDM2. Gudmundsdottir et al (1991) did not find any significant difference in growth rate and colony counts for the 2 media.…”

Section: Discussioncontrasting

confidence: 99%

“…Several studies have also shown culture to be a very sensitive method for detecting R.s. but other investigations have shown certain serological methods to be superior to culture with regard to sensitivity (Cipriano et al 1985, Pascho et al 1987, Sakai et al 1987, Gudmundsdottir et al 1990). Our results showed that culture can fail to confirm a suspicion of BKD based on internal findings, especially in fish that are externally 'healthy looking'.…”

Section: Discussionmentioning

confidence: 98%

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Practical aspects of bacterial culture for the diagnosis of bacterial kidney disease (BKD)

Olsen¹,

Hopp²,

Binde³

et al. 1992

Dis. Aquat. Org.

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A study was made of practical aspects of culture of Renibacterium salrnonjnarum (R.s.) from the kidney of Atlantic salmon Salmo salar. Samples were taken from fish in sea farms affected by outbreaks of bacterial kidney disease (BKD). Only 67% of the samples positive for growth of R.s. on kidney disease medium (KDM2) were positive on selective kidney disease medium (SKDM). Of the samples positive on KDM2, 98 % were identified as positive after 8 wk of incubation a t 15 "C, whereas only 82 "is of the samples positive on SKDlvl were positive at this time Growth of R.s on KDM2 produced more colonies than growth on SKDM No significant differences in isolation frequency were seen betureen culture from anterior and poster~or k~d n e y .If 3 or 4 ~n o c u l a t~o n s were made from each fish, the number of R.s.-poslt~ve f~s h increased by 44 0/,) in the lightly infected population of thls study. Both storage of the sample material on swabs in 2 different niodifications of Stuart's transport medium for 24 h, and freezing a t -20 "C for 3 to 4 mo, reduced the sensitivity of culture from lightly infected samples.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Practical aspects of bacterial culture for the diagnosis of bacterial kidney disease (BKD)

Olsen¹,

Hopp²,

Binde³

et al. 1992

Dis. Aquat. Org.

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“…Fluorescent antibody testing has been well established 2,8,9,11,19 and is used commonly as a screening tool to determine if fish are infected with BKD. Polyclona1 23,25,33 immunoenzyme assays, 26,28,37 and western blot analyses. 17 One advantage of bacterial culture of R. salmoninarum is its sensitivity in detecting low numbers of organisms.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Diagnostic Tests for Bacterial Kidney Disease in Juvenile Steelhead Trout (Oncorhynchus Mykiss)

White

Albregts

1995

J VET Diagn Invest

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In order to accurately diagnose bacterial kidney disease caused by Renibacterium salmoninarum in steelhead trout, kidney tissue from experimentally infected fish was evaluated using a commercially available enzyme-linked immunosorbent assay (ELISA) test kit, fluorescent antibody (FA) testing, bacteriologic culture, and histopathology. Seventy-five steelhead trout were randomly assigned to 1 of 4 groups and intraperitoneally inoculated with 0.15 ml saline ( n = 20), 1 × 1010 organisms/ml ( n = 18), 1 × 108 organisms/ml ( n = 18), or 1 × 106 organisms/ml ( n = 19) of R. salmoninarum. ELISA, FA, and bacteriologic culture were positive for R. salmoninarum from the kidney tissue of the 2 groups infected with the highest doses. Although the ELISA and FA tests were accurate when compared to the bacteriologic culture from the 2 groups infected with the higher doses of the organism, they were less sensitive at the lowest level of inoculum. Histopathology was not specific for this disease; however, all infected fish had a marked proliferative histiocytic interstitial nephritis, characterized by marked expansion of the renal hematopoietic tissue by histiocytes without tissue necrosis. Other microscopic findings included splenitis and myositis (at the injection site) of some fish.

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“…Other investigators have identified soluble antigens in infected fish samples by alternate means of detection such as coagglutination (Kimura and Yoshimizu, 1981;Yozhimizu and Kimura, 1985), counterimmunoelectrophoresis (Cipriano et al, 1985) and peroxidase-antiperoxidase systems (Sakai et al, 1987).…”

Section: Production In Exoerimental Infections Of Salmonmentioning

confidence: 99%

“…A wide variety of serological techniques have been developed for detection of the pathogen, including those employing immunodiffusion (Chen et al, 1974), indirect fluorescent antibody (Bullock and Stuckey 1975), coagglutination (Kimura and Yoshimizu,198 l), counterimmunoelectrophoresis (Cipriano et al, 1985), peroxidase-antiperoxidase (Sakai et al 1987), and antibody capture enzyme-linked immunosorbent assays…”

Section: Introductionmentioning

confidence: 99%

Development of a Vaccine for Bacterial Kidney Disease in Salmon, 1988 Final Report.

Kaattari¹

1989

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Detection of Renibacterium salmoninarum by a modified peroxidase-antiperoxidase(PAP) procedure.

Cited by 18 publications

References 8 publications

Practical aspects of bacterial culture for the diagnosis of bacterial kidney disease (BKD)

Practical aspects of bacterial culture for the diagnosis of bacterial kidney disease (BKD)

Comparison of Diagnostic Tests for Bacterial Kidney Disease in Juvenile Steelhead Trout (Oncorhynchus Mykiss)

Development of a Vaccine for Bacterial Kidney Disease in Salmon, 1988 Final Report.

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