Detection of quorum-sensing<i>N</i>-acyl homoserine lactone signal molecules by bacterial biosensors

Steindler, Laura; Venturi, Vittorio

doi:10.1111/j.1574-6968.2006.00501.x

Cited by 347 publications

(267 citation statements)

References 37 publications

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“…This luteolin effect was found during the exponential growth phase for all carbon sources used. E. meliloti and a few other bacterial species have been reported to produce uncommon AHLs with long acyl chains, containing more than 12 carbons (Gonzalez and Marketon 2003;SanchezContreras et al 2007;Steindler and Venturi 2007;Venturi 2006). It is known that plants produce compounds that mimic AHLs, which are able to interfere with bacterial quorum sensing genes (Gao et al 2003;Hassan and Mathesius 2012;Teplitski et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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Background and aims The establishment of a successful symbiosis between the nitrogen-fixing bacterium Ensifer meliloti and compatible host legumes (Medicago spp.) depends on a complex molecular signal exchange. The early stage of signaling involves the release from plant roots of the flavonoid luteolin, which in turn induces the expression of rhizobia nodulation (nod) genes required for root infection and nodule development. To date, the bacterial response to the luteolin perception has been characterized in detail as far as gene expression is concerned. Nevertheless, despite this molecular information, a global view on E. meliloti phenotypes affected by the plant signal luteolin is still lacking.Therefore, an extensive phenotypic investigation of luteolin effect on the nitrogen-fixing E. meliloti 3001 has been performed. Methods A thousand different growth conditions, including sensitivity to osmolites, pH stresses, antibiotics and toxic compounds, were tested by the application of the high-throughput Phenotype MicroArray (PM) technology, as well as by several dedicated assays to evaluate growth stimulation, motility, biofilm formation, Nacyl homoserine lactones and Indole-3-acetic acid (IAA) production. Results Results revealed that the plant signal luteolin affected a wide spectrum of E. meliloti 3001 phenotypes. E. meliloti 3001 displayed an enhanced resistance phenotype in the presence of luteolin toward a broad set of chemicals including several antibiotics, toxic ions, respiration inhibitors, membrane damagers, DNA intercalants and other potential antimicrobial agents. Moreover, the presence of luteolin significantly reduced overall AHLs production, as well as the lag phase in relation to the starting cellular density, the motility and biofilm formation under nutrient-limited growth conditions. An effect on E. meliloti indole-3-acetic acid (IAA) production was also detected in vitro as a response to luteolin. Conclusions Overall, these findings suggest that the plant signal luteolin triggers a broad response in E. meliloti 3001, which was shown to be dependent on nutritional conditions sensed by the bacterium, pointing out a wide role in modifying rhizobial phenotypes, possibly in relation to plant root association and then symbiotic interaction.Plant Soil (2016) 399:159-178

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Section: Discussionmentioning

confidence: 99%

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

et al. 2015

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“…Plasmid pZLR4 contains a traG::lacZ fusion and traR (Steindler and Venturi, 2007). The plate assay for detection of AHLs was performed as described previously (Hwang et al, 1994).…”

Section: Methodsmentioning

confidence: 99%

Acyl homoserine lactone-based quorum sensing in a methanogenic archaeon

et al. 2012

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Acyl homoserine lactone (AHL)-based quorum sensing commonly refers to cell density-dependent regulatory mechanisms found in bacteria. However, beyond bacteria, this cell-to-cell communication mechanism is poorly understood. Here we show that a methanogenic archaeon, Methanosaeta harundinacea 6Ac, encodes an active quorum sensing system that is used to regulate cell assembly and carbon metabolic flux. The methanogen 6Ac showed a cell density-dependent physiology transition, which was related to the AHL present in the spent culture and the filI gene-encoded AHL synthase. Through extensive chemical analyses, a new class of carboxylated AHLs synthesized by FilI protein was identified. These carboxylated AHLs facilitated the transition from a short cell to filamentous growth, with an altered carbon metabolic flux that favoured the conversion of acetate to methane and a reduced yield in cellular biomass. The transcriptomes of the filaments and the short cell forms differed with gene expression profiles consistent with the physiology. In the filaments, genes encoding the initial enzymes in the methanogenesis pathway were upregulated, whereas those for cellular carbon assimilation were downregulated. A luxI-luxR ortholog filI-filR was present in the genome of strain 6Ac. The carboxylated AHLs were also detected in other methanogen cultures and putative filI orthologs were identified in other methanogenic genomes as well. This discovery of AHL-based quorum sensing systems in methanogenic archaea implies that quorum sensing mechanisms are universal among prokaryotes.

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“…CV026 which was used for C4 to C8 AHL detection develops purple pigmentation when exposed to exogenous AHLs with acyl chains shorter than C10 except C4-3-oxo-BHL (McClean et al, 1997;Steindler and Venturi, 2007). NTL4 was used for the detection of AHLs with acyl chains longer than C10 as it can respond to 3-unsubstituted and substituted AHLs with exception of C4-AHL (Cha et al, 1998;Farrand et al, 2002; Zhu et al, 2003;Kawaguchi et al, 2008).…”

Section: Detection Of Qq Activitiesmentioning

confidence: 99%

“…Chromobacterium violaceum CV026 (ATCC 31532, ATCC, USA) was used as the biosensor strain to test QQ activity of the isolates against C4-C8 AHLs and cultivated in Luria-Bertani (LB) medium (McClean et al, 1997;Steindler and Venturi, 2007). Agrobacterium tumefaciens NTL4 (pZLR4, Luo et al, 2003) served as the biosensor for C8 -C12 AHLs and cultivated in AB mannitol medium (ATCC medium 1691).…”

Section: Bacterial Strains and Mediamentioning

confidence: 99%

Characterization of Quorum-Quenching Bacteria Isolated from Biofouled Membrane Used in Reverse Osmosis Process

Moon¹,

Huang²,

Choi³

et al. 2014

The Korean Journal of Microbiology

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Acyl homoserine lactone (AHL) lactonase has been proved to be the AHL-degrading enzyme with the highest substrate specificity for AHL molecules and has shown a considerable potential as low-cost and efficient quorum quenching (QQ) technique. However, few studies focused on its inhibitory effect on biofilm formation which is also a quorum sensing (QS)-regulated phenomenon. In this study, QQ activity of six isolates from biofouled reverse osmosis membranes was studied using Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NTL4 as biosensors under various conditions. All of the isolates belonged to the genus Bacillus and showed QQ activity regardless of the acyl chain length or substitution of AHL molecule. The isolates were capable of significantly inhibiting biofilm formation (46.7-58.3%) by Pseudomonas aeruginosa PAO1 and produced heat-sensitive extracellular QQ substances. The LC-MS analysis of the QQ activity of a selected isolate, RO1S-5, revealed the degradation of N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12 AHL) and the production of corresponding acyl homoserine (3-oxo-C12-HS), which indicated the activity of AHL lactonase. The broad AHL substrate range and high substrate specificity suggested that the isolate would be useful for the control of biofilm-related pathogenesis and biofouling in industrial processes. Keywords:Pseudomonas aeruginosa, AHL lactonase, biofilm, quorum quenching, quorum sensing *For correspondence. E-mail: ysoh@mju.ac.kr; Tel.: Quorum sensing (QS) allows bacteria to perceive the density of the surrounding bacterial populations using signal molecules such as N-acyl homoserine lactone (AHL) and to coordinately respond to this information by regulating expression of various genes (Smith and Iglewski, 2003a). QS has been known to play significant roles in many biological processes such as virulence factor production, bioluminescence, antibiotic production, sporulation, and biofilm formation (Novick and Geisinger, 2008;Ng and Bassler, 2009; Williams and Cámara, 2009).Among these, biofilm formation is one of the major contributors to the pathogenesis of many clinically important bacteria, and the loss of efficiency in medical, industrial, and environmental processes such as medically-implanted devices, oil drilling, paper production, food processing, fish farming, and membrane separation processes (Donlan, 2001; Defoirdt et al., 2004; Adonizio et al., 2008; Ammor et al., 2008; Wevers et al., 2009; Van Houdt and Michiels, 2010). Biofilm is not only ubiquitous and costly but also endows the cells with resistance to many antimicrobial agents, which has made it difficult to suggest prevalent techniques to control biofilm development (Davies et al., 1998;Stewart and Costerton, 2001;Hoffman et al., 2005). Therefore, there has been a high demand for effective strategies to control biofilm formation, and targeting the QS regulatory mechanism has been a highly considerable candidate due to the critical role of QS in biofilm formation (Davies et al., 1998;Smith and Iglewski,...

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Detection of quorum-sensingN-acyl homoserine lactone signal molecules by bacterial biosensors

Cited by 347 publications

References 37 publications

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Effect of the plant flavonoid luteolin on Ensifer meliloti 3001 phenotypic responses

Acyl homoserine lactone-based quorum sensing in a methanogenic archaeon

Characterization of Quorum-Quenching Bacteria Isolated from Biofouled Membrane Used in Reverse Osmosis Process

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