Detection of ptc in Archival Formalin-Fixed, Paraffin-Embedded Tissues

Rk, Martin; Kt, Archer; Rm, Tuttle

doi:10.1097/00019606-199412000-00004

Cited by 10 publications

(2 citation statements)

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“…The low incidence of 5%, initially reported based on RT-PCR alone (31), increased to 47% when RT-PCR was followed by hybridization (30). Lanes 1,5,7,9,10,13, and 14 were positive for two types, and lanes 2 and 11 were positive for three types, of rearrangements. In our study, we also found that RT-PCR followed by hybridization was superior to RT-PCR alone for demonstrating ret rearrangements.…”

Section: Discussionmentioning

confidence: 97%

“…For paraffin-embedded tissues, total RNA was extracted as described previously (13), with the following modifications. Briefly, tissue was deparaffinized with three 10-min incubations of 1 mL dipentene (Histolene, Fronine Pty Ltd, Riverstone, New South Wales, Australia) at room temperature, followed by graded (100%, 80%, 50%) ethanol washes and extraction with diethylpyrocarbonate-treated water.…”

Section: Rna Extractionmentioning

confidence: 99%

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Prevalence and Distribution of ret/ptc 1, 2, and 3 in Papillary Thyroid Carcinoma in New Caledonia and Australia1

Chua¹,

Wu²,

Tran

et al. 2000

The Journal of Clinical Endocrinology &Amp; Metabolism

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The world's highest incidence of thyroid cancer has been reported among females in New Caledonia, a French overseas territory in the Pacific located between Australia and Fiji. To date, no molecular genetic studies in this population are available. Over the past few years, the oncogenic rearrangement of the ret protooncogene (ret/ptc) has been studied in papillary carcinomas in different populations. In this study, we investigated the prevalence and distribution of ret/ptc1, 2, and 3 in papillary thyroid carcinoma from the New Caledonian population and compared the pattern with that of an Australian population. Fresh-frozen and paraffin-embedded papillary carcinomas from 27 New Caledonian and 20 Australian patients were examined for ret rearrangements by means of RT-PCR with primers flanking the chimeric region, followed by hybridization with radioactive probes. ret/ptc was present in 70% of the New Caledonian and in 85% of the Australian samples. Multiple rearrangements were detected and confirmed by sequencing in 19 cases, 4 of which had 3 types of rearrangements in the same tumor. This study demonstrates a high prevalence of ret/ptc in New Caledonian and Australian papillary carcinoma. The findings of multiple ret/ptc in the same tumor suggest that some thyroid neoplasms may indeed be polyclonal.

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Section: Discussionmentioning

confidence: 97%

Section: Rna Extractionmentioning

confidence: 99%

Prevalence and Distribution of ret/ptc 1, 2, and 3 in Papillary Thyroid Carcinoma in New Caledonia and Australia1

Chua¹,

Wu²,

Tran

et al. 2000

The Journal of Clinical Endocrinology &Amp; Metabolism

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Ras mutations are uncommon in sporadic thyroid cancer in children and young adults

Fenton

Anderson

Lukes

et al. 1999

J Endocrinol Invest

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Mutations in the ras genes (H-ras, K-ras, and N-ras) occur in 10-15% of all human cancers, and commonly arise from single base substitutions at codons 12, 13, or 61. Although ras mutations have been found in adult thyroid cancers, they were absent from the two studies which examined childhood thyroid cancers. Both studies included only children with radiation induced thyroid cancer, and it remains unclear if ras mutations occur in children without radiation exposure. To answer this question, we examined archival tissue blocks from 31 children with papillary thyroid cancer (PTC) 4 with follicular thyroid cancer (FTC), 2 with medullary thyroid cancer (MTC), and 1 with lymphoma (LYM). Only 1 patient with PTC had previous radiation exposure. Genomic DNA was extracted and used for PCR amplification of the ras genes. The PCR products were analyzed by oligospecific hybridization for mutations at codons 12, 13, and 61. Two of the PTCs (6.5%) contained ras mutations. Both patients had class II disease and no history of previous radiation exposure. One patient subsequently developed bone and lung metastases. The patient with lymphoma also had a ras mutation (N-61), but ras mutations were absent from all FTC and MTC. These results suggest that ras mutations are uncommon in spontaneous childhood thyroid cancer, but occur with a frequency similar to that found in previous reports of adult differentiated thyroid cancers. The number of subjects was too small to determine if ras mutations are more common in patients with aggressive papillary thyroid cancer.

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The use of the polymerase chain reaction to detect bacteria in amniotic fluid in pregnancies complicated by preterm labor

Markenson¹,

Martin²,

Tillotson-Criss³

et al. 1997

American Journal of Obstetrics and Gynecology

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Detection of ptc in Archival Formalin-Fixed, Paraffin-Embedded Tissues

Cited by 10 publications

References 0 publications

Prevalence and Distribution of ret/ptc 1, 2, and 3 in Papillary Thyroid Carcinoma in New Caledonia and Australia1

Prevalence and Distribution of ret/ptc 1, 2, and 3 in Papillary Thyroid Carcinoma in New Caledonia and Australia1

Ras mutations are uncommon in sporadic thyroid cancer in children and young adults

The use of the polymerase chain reaction to detect bacteria in amniotic fluid in pregnancies complicated by preterm labor

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