Detection of proteins in wheat flour extracts that bind human IgG, IgE, and mouse monoclonal antibodies

Theobald, K.; Thiel, H; Kallweit, C.; Ulmer, W. T.; König, Wolfgang

doi:10.1016/0091-6749(86)90035-7

Cited by 35 publications

(16 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…But IgE and IgG binding was more frequent in bakers with airway hypersensitivity as compared to donors with out any allergic symptoms. These data are in line with the results reported previously by Theobald et al [15] and Kiefer et al [16] who demonstrated Ig binding towards wheat flour components from the sera of nonallergic donors. Bakers who reported dyspnea and cough at the work-place expressed more fre quently IgE with specificity towards the 47-, 17-and 15-kD bands than nonallergic donors.…”

Section: Discussionsupporting

confidence: 83%

Western Blot Analysis of Water-Soluble Wheat Flour <i>(Triticum vulgaris) </i>Allergens

Pfeil¹,

Schwabl

et al. 1990

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Water-soluble wheat flour allergens were analyzed using the western blot technique. The sera of 130 bakers (42 with wheat flour allergy, 88 without allergy) were analyzed for IgE, IgG, and IgG4 binding towards wheat flour allergens. Three major allergens with molecular weights of 47, 17, and 15 kD were identified (Tri v Bd 47, Tri v Bd 17, and Tri v Bd 15; IUIS allergen nomenclature) by their ability to bind IgE from approximately 50% of the allergic sera. The specificity of the IgG was mostly directed against three high molecular weight components (134, 122, and 98 kD). In contrast, IgE and IgG4 was found frequently against the major allergens. 40% of healthy donors versus 12% of the asthmatic bakers had no detectable IgG4 against wheat flour extract. The biological activity of the water-soluble wheat flour components has been shown by their ability to induce histamine release from basophil leukocytes obtained from patients with bakers’ asthma. The release of histamine from basophil leukocytes significantly correlated with the presence of IgE antibodies against wheat flour components of 47, 17 and 15 kD. A regulatory role of wheat flour specific IgG4 in allergic diseases could not be evaluated by western blot analysis.

show abstract

Section: Discussionsupporting

confidence: 83%

Western Blot Analysis of Water-Soluble Wheat Flour <i>(Triticum vulgaris) </i>Allergens

Pfeil¹,

Schwabl

et al. 1990

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although a number of allergens, including some additives [4], have been involved in baker's asthma, the salt-soluble proteins of flour dust seem to be the most relevant ones [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

A major baker's asthma allergen from rye flour is considerably more active than its barley counterpart

García‐Casado¹,

Armentia²,

Sánchez‐Monge³

et al. 1995

FEBS Letters

View full text Add to dashboard Cite

A rye flour protein of about 13.5 kDa, as well as its barley homologue, have been isolated. The rye component was recognized in vitro by IgE of allergic patients and provoked positive responses in 15 out of 21 baker's asthma patients (71%) when skin prick tests were performed. Its barley homologue showed no detectable in vitro reactivity and caused positive responses in only one-third of patients. Although no inhibitory activity against different a-amylases or trypsin was found for these two proteins, their N-terminal sequencing revealed considerable similarity to several members of the cereal ~x-amylase/trypsin inhibitor family.

show abstract

“…Theobald et al [10]studied the biologic significance of the extracted cereal proteins. These investigators demonstrated the release of histamine in vitro by basophil leukocytes obtained from patients suffering from asthma by adding aqueous wheat extract.…”

Section: Introductionmentioning

confidence: 99%

Pharmacological Studies of the Effect of Wheat Grain Extract

Schachter

Zuśkin²,

Rienzi³

et al. 2004

Respiration

View full text Add to dashboard Cite

Background: Agricultural farm workers exposed to wheat grain dust are at risk of developing respiratory abnormalities. The pathogenesis of this injury is only partially understood. Objectives: To determine the effect of wheat grainextract on isolated guinea pig tracheal smooth muscle. Methods: In the current study, pharmacologic properties of wheat grain extract (WGE) were tested using guinea pig tracheas studied in vitro. Dose-related contractions of nonsensitized guinea pig trachea were demonstrated using these extracts. Pharmacologic studies were performed by pretreating guinea pig tracheal tissue with drugs known to modulate smooth muscle contraction: atropine 10^–6M, indomethacin 10^–6M, pyrilamine 10^–6M, acivicin 10^–5M, nordihydroguaretic acid (NDGA) 10^–5M, bromophenacyl bromide (BPB) 10^–5M, 3,4,5-trimethoxybenzoic acid-8-(diethylamino)- octyl ester TMB8 10^–5M, captopril 10^–5M and capsaicin 5 × 10^–6M. Results: WGE causes a dose-dependent constriction of guinea pig tracheal smooth muscle. Atropine, pyrilamine, TMB8 and acivicin significantly reduced the contractile effects of the WGE. Inhibition of contraction by blocking of other mediators was significant but less complete. Conclusion: We conclude that WGE causes a dose-related constriction of airway smooth muscle by nonimmunological mechanisms involving a variety of airway mediators and possibly cholinergic receptors.

show abstract

Detection of proteins in wheat flour extracts that bind human IgG, IgE, and mouse monoclonal antibodies

Cited by 35 publications

References 16 publications

Western Blot Analysis of Water-Soluble Wheat Flour <i>(Triticum vulgaris) </i>Allergens

Western Blot Analysis of Water-Soluble Wheat Flour <i>(Triticum vulgaris) </i>Allergens

A major baker's asthma allergen from rye flour is considerably more active than its barley counterpart

Pharmacological Studies of the Effect of Wheat Grain Extract

Contact Info

Product

Resources

About