Detection of prion after decontamination procedures: comparative study of standard Western blot, filter retention and scrapie-cell assay

Solassol, Jérôme; Arlotto, Marie; Lehmann, Sylvain

doi:10.1016/j.jhin.2004.03.005

Cited by 17 publications

(20 citation statements)

References 14 publications

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“…Although more than a 5-log 10 inactivation was achieved, the exposure time was up to 2 weeks. Other studies also demonstrated that hydroxyl radicals inactivated infectious prions, more or less, with exposure times from 30 min to several hours (23,56,57,59,60,62). However, none of these studies investigated the reaction kinetics.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of Ozone Inactivation of Infectious Prion Protein

Ding

Neumann

Price

et al. 2013

Appl Environ Microbiol

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eThe kinetics of ozone inactivation of infectious prion protein (PrP Sc , scrapie 263K) was investigated in ozone-demand-free phosphate-buffered saline (PBS). Diluted infectious brain homogenates (IBH) (0.01%) were exposed to a predetermined ozone dose (10.8 ؎ 2.0 mg/liter) at three pHs (pH 4.4, 6.0, and 8.0) and two temperatures (4°C and 20°C). The inactivation of PrP Sc was quantified by determining the in vitro destruction of PrP Sc templating properties using the protein misfolding cyclic amplification (PMCA) assay and bioassay, which were shown to correlate well. The inactivation kinetics were characterized by both Chick-Watson (CW) and efficiency factor Hom (EFH) models. It was found that the EFH model fit the experimental data more appropriately. The efficacy of ozone inactivation of PrP Sc was both pH and temperature dependent. Based on the EFH model, CT (disinfectant concentration multiplied by contact time) values were determined for 2-log 10 , 3-log 10 , and 4-log 10 inactivation at the conditions under which they were achieved. Our results indicated that ozone is effective for prion inactivation in ozone-demand-free water and may be applied for the inactivation of infectious prion in prion-contaminated water and wastewater.

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Section: Discussionmentioning

confidence: 99%

Kinetics of Ozone Inactivation of Infectious Prion Protein

Ding

Neumann

Price

et al. 2013

Appl Environ Microbiol

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“…Therefore, an inactivation of infectious prion protein by microbial PrP Sc degrading processes during digestion is assumed. As a future task, this correlation will be validated by in vivo hamster bioassays, as Solassol et al [17] found that reduced PrP Sc levels in immunoblots do not correspond with in vivo data. However, the in vitro prion protein degradation procedure does not yet allow a final conclusion about the real in vivo conditions.…”

Section: Discussionmentioning

confidence: 99%

Degradation of scrapie associated prion protein (PrP^Sc) by the gastrointestinal microbiota of cattle

Scherbel¹,

Pichner²,

Groschup

et al. 2006

Vet. Res.

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-A food-borne origin of the transmission of bovine spongiform encephalopathy (BSE) to cattle is commonly assumed. However, the fate of infectious prion protein during polygastric digestion remains unclear. It is unknown at present, whether infectious prion proteins, considered to be very stable, are degraded or inactivated by microbial processes in the gastrointestinal tract of cattle. In this study, rumen and colon contents from healthy cattle, taken immediately after slaughter, were used to assess the ability of these microbial consortia to degrade PrP Sc . Therefore, the consortia were incubated with brain homogenates of scrapie (strain 263K) infected hamsters under physiological anaerobic conditions at 37• C. Within 20 h, PrP Sc was digested both with ruminal and colonic microbiota up to immunochemically undetectable levels. Especially polymyxin resistant (mainly gram-positive) bacteria expressed PrP Sc degrading activity. These data demonstrate the ability of bovine gastrointestinal microbiota to degrade PrP Sc during digestion.transmissible spongiform encephalopathy / prion / degradation / microbiota / gastrointestinal tract

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“…24 Indeed, an immunoblot assay is not sufficiently sensitive to evaluate the efficiency of a product. 25 Animal inoculations appear to be a standardizable method for the evaluation of prion inactivation in a sample, providing that a 15-month incubation is acceptable, in order to guarantee the total absence of residual infectivity. This is an absolute requirement to validate a real 'no-risk treatment' for sterilization or disinfection applications.…”

Section: Discussionmentioning

confidence: 99%

Quantitative evaluation of prion inactivation comparing steam sterilization and chemical sterilants: proposed method for test standardization

Vadrot¹,

Darbord²

2006

Journal of Hospital Infection

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Detection of prion after decontamination procedures: comparative study of standard Western blot, filter retention and scrapie-cell assay

Cited by 17 publications

References 14 publications

Kinetics of Ozone Inactivation of Infectious Prion Protein

Kinetics of Ozone Inactivation of Infectious Prion Protein

Degradation of scrapie associated prion protein (PrP^Sc) by the gastrointestinal microbiota of cattle

Quantitative evaluation of prion inactivation comparing steam sterilization and chemical sterilants: proposed method for test standardization

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Detection of prion after decontamination procedures: comparative study of standard Western blot, filter retention and scrapie-cell assay

Cited by 17 publications

References 14 publications

Kinetics of Ozone Inactivation of Infectious Prion Protein

Kinetics of Ozone Inactivation of Infectious Prion Protein

Degradation of scrapie associated prion protein (PrPSc) by the gastrointestinal microbiota of cattle

Quantitative evaluation of prion inactivation comparing steam sterilization and chemical sterilants: proposed method for test standardization

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Degradation of scrapie associated prion protein (PrP^Sc) by the gastrointestinal microbiota of cattle