Detection of Prethrombin 1 in Human Blood Plasma

Korolova, Daria; Chernyshenko, Volodymyr; Platonova, T. M.; Chernyshenko, Tamara; Lugovskoy, E. V.

doi:10.9734/ibrr/2016/24683

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…Results of prothrombin and ecamulin tests were presented as the PI and EI as calculated by the formulas: EI = An/Ap; PI = An/Ap, where An is the blood plasma thrombin activity of a healthy control animal; Ap is the blood plasma thrombin activity of exposed animal. The difference between PI and EI that was more than 10% indicated the accumulation of FIFPs above 12 µg/ml, that was preliminarily assumed as evidence of thrombotic complications [18].…”

Section: Methodsmentioning

confidence: 93%

Blood coagulation parameters in rats with acute radiation syndrome receiving activated carbon as a preventive remedy

Chernyshenko¹,

Snezhkova²,

Mazur³

et al. 2019

Ukr.Biochem.J

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radiation-induced coagulopathy (rIC) is one of the major causes of death during acute radiation syndrome (arS). The aim of this study was to characterize the responses of the hemostasis system to arS of a moderate level on the 1 st and 9 th days after irradiation. We aimed to identify molecular markers of the blood coagulation system that are most affected by ARS and to estimate the enterosorption effect on the development of irradiation-induced changes. Platelet aggregation rate, activated partial thromboplastin time (aPTT) and fibrinogen concentration were determined by standard methods. Level of protein C (PC) was measured using chromogenic substrate S2366 (p-glu-Pro-arg-pNa) and agkistrodon halys halys snake venom activating enzyme. Functionally inactive forms of prothrombin (FIFPs) were determined using two activators in parallel-thromboplastin or prothrombin activator from echis multisqumatis venom. rats of both irradia ted groups had a higher risk of intravascular clotting in comparison to both control groups. Statistically significant shortening of clotting time in the APTT test (24 ± 4 s vs. 33 ± 5 s) and increased fibrinogen concentration (4.2 ± 0.6 mg/ml vs. 3.2 ± 0.3 mg/ml) were detected. Both parameters were normalized on the 9 th day after irradiation. however the platelet count was decreased (0.3•10 6 ± 0.05•10 6 1/μl vs. 0.145•10 6 ± 0.04•10 6 1/μl) due to the impaired megakaryocytic function. The level of PC was decreased after X-ray irradiation (70 ± 10%) and partly restored on the 9 th day after irradiation (87 ± 10%). administration of activated carbon (aC) inhibited the drop in the PC concentration after X-ray irradiation (86 ± 15%) and accelerated its restoration on the 9th day (103 ± 14%). The statistically significant accumulation of FIFPs was detected in blood plasma of irradia ted rats at the 1 st and 9 th days after irradiation. No FIFPs were found in any irradiated rat treated with aC. Characterization of the hemostasis system of rats that were exposed to a semilethal dose of X-rays allowed us to select parameters that can be used for monitoring of arS development. apart of from basic coagulation tests (APTT) and the measurement of platelet aggregation, fibrinogen and protein C level we can recommend the determination of FIFPs as a useful tool for estimation of the hemostasis response after irradiation with X-rays. This test indicates the intravascular thrombin generation and can help predict thrombotic complication or disseminated intravascular coagulation. determination of FIFPs in blood plasma of irradia ted rats allowed us to study the enterosorption effect on the development of irradiationinduced changes. It was shown that enterosorption with aC prevented accumulation of FIFPs which appears to be a newly discovered antithrombotic effect of therapy with AC. ARS influenced hemostasis by inducing thrombin generation (indicated by FIFPs generation), lowgrade inflammation (indicated by PC concentration decrease) and thrombocytopenia. Enterosorption with AC minimizes inflammation and pr...

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Section: Methodsmentioning

confidence: 93%

Blood coagulation parameters in rats with acute radiation syndrome receiving activated carbon as a preventive remedy

Chernyshenko¹,

Snezhkova²,

Mazur³

et al. 2019

Ukr.Biochem.J

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“…Результати епідеміологічних досліджень свідчать про наявну залежність концентрації фібриногену у плазмі крові та розвитку інсульту та інфар-кту [11]. Це пов'язано із притаманними йому здатністю експоненціально впливати на в'язкість крові, активувати процеси агрегації тромбоцитів та на інші білки системи згортання (фібронектин, колаген, тромбоспондин) [2]. На цьому тлі важлива роль належить антикоагулянтній системі, природним антикоагулянтомпротеїном С, який, окрім всього, у фізіологічних умовах бере участь і в регуляції системи фібринолізу.…”

Section: обговоренняunclassified

“…During five years of follow up there were 13 deaths (14.8%), 7 among males and 6 among females, caused by thrombotic complications. The main thrombotic complications were myocardial infarction (6), ischemic stroke (4), mesenteric thrombosis (2)…”

Section: Some Indicators Of Hemostasis and Mortalitymentioning

confidence: 99%

Some indicators of hemostasis and mortality from thrombotic complications in patients with CKD stage VD who were treated by programmed hemodialysis (five-year follow-up)

Сторожук¹,

Seleznyova²,

Сторожук³

et al. 2021

Rep. of Vinnytsia Nation. Med. Univ.

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Annotation. Thrombotic complications in patients with stage VD chronic kidney disease (CKD), treated by program hemodialysis is one of the causes of high mortality in this category of patients; its major pathogenetic component proved to be hemostatic system disturbances characterized by systemic activation of blood clotting process leading to the development of thrombophilia. Objective – to study the biochemical molecular markers of hemostasis in patients with stage VD CKD, treated by program hemodialysis, and to determine their long-term effect on the development of thrombotic complications and mortality. The study included 88 patients (52 males and 36 females) aged 26 to 65 years with stage VD CKD, treated by program hemodialysis. The patients were followed up for five years. Soluble fibrin (sF) level was determined by two-site enzyme-linked immune-sorbent quantitative assay; D-dimer – by enzyme immunoassay using specific monoclonal antibodies to D-dimer epitopes; protein C (pC) activity in blood plasma was estimated by its activation with copperhead snake venom followed by spectrophotometry with wavelength 405 nm. Blood plasma fibrinogen was determined using thrombin-like enzyme Antsistron-H by spectrophotometry with wavelength 280 nm. Blood plasma fibrinolytic activity was evaluated by the relationship between D-dimer and soluble fibrin. Processing of materials was carried out using the methods of variation statistics and correlation analysis. During five years of follow up there were 13 deaths (14.8%), 7 among males and 6 among females, caused by thrombotic complications. The main thrombotic complications were myocardial infarction (6), ischemic stroke (4), mesenteric thrombosis (2) and disseminated intravascular coagulation syndrome (DIC) (1) (according to pathomorphological data). The patients of general group were found to have significantly increased sF level, decreased pC as compared to the control group, as well as twofold increase of fibrinogen concentration along with decreased D-dimer/sF ratio and no response of D-dimer to increased soluble fibrin level. The tendency of D-dimer/sF ratio to increase in those who died because of thrombotic complications could be indicative of microthrombosis with formation of fibrin derivatives along with mild activation of fibrinolysis. Correlation relationships between soluble fibrin and D-dimer, fibrinogen and protein C in general group were assessed, and the following data were obtained: medium direct relationship between soluble fibrin and D-dimer (r= 0.56) (p<0.001), and absence of correlation with fibrinogen (r= -0.12) and protein C (r= -0.10) (p˃0.1). Besides, strong positive correlation was demonstrated between D-dimer and soluble fibrin (r= 0.87) (p<0.001), and moderately negative one between D-dimer and protein C (r= -0.21) (p<0.05). It should be noted that in patients with thrombotic complications, positive correlations between soluble fibrin and D-dimer become stronger (r= 0.51) (p<0.001), as well as negative ones between soluble fibrin and protein C (r= -0.22) (p<0.05). Depressive state of anticoagulant component along with activation of coagulation factors are supposed to be one of the indicators predicting thrombophilia in this category of patients.

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“…Ecamulin (prothrombin activator from echis multisqumatis venom) is an enzyme that activates prothrombin, descarboxy-prothrombin and prethrombin 1. So activation by ecamulin allowed us to determine total prothrombin level [22]. Thrombin generation induced by ecamulin was measured by chromogenic substrate assay using thrombin-specific S2238 (H-D-Phe-Pip-Arg-pNA) as previously described [23].…”

Section: Methodsmentioning

confidence: 99%

Blood coagulation and aortic wall integrity in rats with obesity-induced insulin resistance

Dziuba¹

2018

Ukr.Biochem.J

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Obesity is an important factor in pathogenesis of disorders caused by chronic inflammation. Diet-induced obesity leads to dyslipidemia and insulin resistance (Ir) O besity is recognized as an important factor in the pathogenesis of metabolic disorders. Metabolic syndrome which is related to weight gain, specifically intra-abdominal fat accumulation, is a known risk factor for early cardiovascular diseases. The reason for such a dependence is clear from the other name for the metabolic syndrome; it is also known as 'the deadly quartet'

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Detection of Prethrombin 1 in Human Blood Plasma

Cited by 4 publications

References 17 publications

Blood coagulation parameters in rats with acute radiation syndrome receiving activated carbon as a preventive remedy

Blood coagulation parameters in rats with acute radiation syndrome receiving activated carbon as a preventive remedy

Some indicators of hemostasis and mortality from thrombotic complications in patients with CKD stage VD who were treated by programmed hemodialysis (five-year follow-up)

Blood coagulation and aortic wall integrity in rats with obesity-induced insulin resistance

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